Click to read about some of our current projects:
Click to read about some of our previous projects:
Overview: Bioinorganic Chemistry in the Franz Lab
Iron, copper, zinc, and manganese are mineral nutrients that are required as catalytic and structural cofactors for numerous proteins that execute essential life processes. At the same time, these metals must be tightly controlled, as misregulation and overload of these metals can lead to oxidative stress, protein disruption, and general deleterious effects to the cell.
Research in the Franz Lab centers on elucidating the structural and functional consequences of metal ion coordination in biological systems and developing therapeutic strategies that use manipulation of metal homeostasis as a mechanism of action. We also investigatebehavior of metal coordination complexes subjected to mechanical force. Our projects explore several different areas of metallobiology:
Metals in cancer. Cancer cells have considerably different metallomes than normal cells. In particular, prostate cancer overexpresses several important copper trafficking proteins and recruits high levels of copper. Our interest lies in selectively targeting this metal ion pool by developing prochelators that are activated in the prostate cancer microenvironment.
Metals in fungal pathogens.The Franz lab has two projects at the intersection of fungal biology and metal homeostasis. In the first project, we are addressing how antifungal therapy impacts the utilization of metals by the fungal pathogen Candida albicans, and how this pathogen uses metal-specific responses to adapt to and recover from drug stress. Our second fungal project aims to characterize histatin-5, an antifungal peptide produced in the salivary glands that acts as part of the host’s innate defense system. We are exploring the mechanism by which antifungal activity of histatin-5 against C. albicansincreases in the presence of copper.
Metals in bacterial pathogens.With the growing threat of antibiotic drug resistance, new antibacterial treatment strategies are urgently needed. Our lab is working to develop prodrugs that are activated by drug-resistant bacteria to release metal chelators, such as the ionophore pyrithione. We are also investigating the effects of these chelators on metal homeostasis within the bacterial cell.
Metals in polymers.The Franz Lab is also exploring metals in non-biological systems. Stretching polymers to their breaking point will eventually cause bond scission and polymer degradation. Forces below the rupture limit can induce constructive chemical changes to stress-responsive moieties, or mechanophores. The insertion of coordination complexes as mechanophores into polymer structures helps to explorehow mechanical force delivered at the molecular level can distort the coordination environment about a metal center in ways that access unique structural, electronic, and reactivity profiles.
Cancer cells have been shown to have considerably different metallomes than normal cells. In particular, prostate cancer overexpresses several important copper trafficking proteins and recruits high levels of copper, making it more sensitive towards drugs like disulfiram. Our interest lies in selectively targeting this metal ion pool by developing prochelators that are activated in the cancer microenvironment. Towards this end we have developed a copper prochelator, GGTDTC, to target an aggressive prostate cancer cell line. The GGT enzyme overexpressed in prostate cancer induces release of the chelator dithiocarbamate from the prochelator, which can then form a toxic copper(II) complex. We have reported the efficacy and selectivity of the prochelator GGTDTC for prostate cancer over a prostate normal cell line. We are interested in expanding the prochelator/prodrug library with varying specificity towards different cancers and conditions with altered metal ion homeostasis like Wilson’s disease.
Current lab members working on this project: Azim
- Bakthavatsalam, S.; Sleeper, M. L.; Dharani, A.; George, D. J.; Zhang, T.; Franz, K. J., Leveraging gamma-glutamyl transferase to direct cytotoxicity of copper dithiocarbamates against prostate cancer cells. Angewandte Chemie International Edition 2018, 57 (39), 12780-12784.
The growing threat of drug-resistant bacteria has compelled the Franz Lab to develop therapeutics with new modes of action involving metal chelation. Copper has been used as an antimicrobial agent since ancient times, and compounds with copper-binding capabilities, such as pyrithione, have demonstrated promising antimicrobial activity. However, non-specific metal chelation leads to systemic disruption of metal homeostasis and undesired side effects, limiting the use of chelators as therapeutics. A way to direct metal chelation to the desired microenvironment is the use of prochelators. Prochelators are molecules that are unable to bind metals, but can be triggered by a stimulus, such as an enzyme, to release a chelating moiety. They remain inactive until triggered, allowing them to have activity only at the site of interest. We have reported the antibacterial effects and investigated the role of copper in the mode of action of PcephPT, a prochelator triggered by β-lactamase to release pyrithione in the microenvironment of drug-resistant bacteria. We are currently continuing our investigation into the mode of action of copper and pyrithione in this prochelator strategy. We are also developing next-generation prochelators with new modes of action and improved selectivity for bacteria with certain drug resistance profiles.
Current lab members working on this project: Jacqueline, Abbey, Elena, Kevin, Christine, Tyler
- Zaengle-Barone, J. M.; Jackson, A. C.; Besse, D. M.; Becken, B.; Arshad, M.; Seed, P. C.; Franz, K. J., Copper Influences the Antibacterial Outcomes of a β-Lactamase-Activated Prochelator against Drug-Resistant Bacteria. ACS Infect Dis2018,4(6), 1019-1029.
Histatins are a family of histidine-rich antimicrobial peptides that are produced in the salivary glands and act as a part of the host’s innate defense system. Histatins exhibit potent antifungal activity, specifically for the opportunistic fungal pathogen Candida albicans. These peptides are known to bind metals in vitro; however, these interactions are not well understood and their effects on the peptides’ antifungal activity are not well characterized. Our lab has shown that the antifungal activity of histatin-5 against C. albicans increases in the presence of copper. Spectroscopic methods were used to show that specific histidine residues on the peptide were responsible for the peptide’s copper binding abilities and subsequent fungicidal activity. Currently, we are working to further elucidate the mechanism of action of histatin-5 and explore how this mechanism may be affected by various metals using analytical and spectroscopic techniques and microscopy.
Current lab members working on this project: Joanna, Jaewon, Anna
- Conklin, S. E.; Bridgman, E. C.; Su, Q.; Riggs-Gelasco, P.; Haas, K. L.; Franz, K. J., Specific Histidine Residues Confer Histatin Peptides with Copper-Dependent Activity against Candida albicans. Biochemistry 2017, 56 (32), 4244-4255.
Iron (Fe) and copper (Cu) play important roles in C. neoformans virulence, but these metals are also potent toxins if misappropriated. Based on the need of C. neoformans to utilize and maintain tight control of these two metals for survival and virulence, we hypothesized that small molecules capable of manipulating Fe and/or Cu bioavailability may have antimicrobial activity. To investigate this possibility, we tested a panel of metal chelating agents for their ability to inhibit C. neoformans growth under a variety of metal growth environments. We also characterized chemical and physical properties of these compounds, allowing us to correlate these properties with metal-dependent antifungal activity.
Current lab members working on this project: Lizzie
- Helsel, M. E.; White, E. J.; Razvi, S. Z. A.; Alies, B.; Franz, K. J., Chemical and functional properties of metal chelators that mobilize copper to elicit fungal killing of Cryptococcus neoformans. Metallomics 2017, 9 (1), 69-81.
ROS-Triggered Iron Chelators
A locally elevated iron load in brain regions affected by Parkinson’s disease insinuates iron as a source of cell-damaging hydroxyl radicals. While chelating agents are promising, there are significant challenges in developing agents that sequester deleterious iron without altering the distribution of healthy metal ions. Our strategy is to design pro-chelators that have little to no affinity for metal ions until a protective mask is selectively removed by hydrogen peroxide, a reactive oxygen species (ROS) that combines with iron to form hydroxyl radicals. Disease conditions therefore activate the chelating agent. In our first-generation pro-chelator shown below, a boronic ester conceals a latent phenolic oxygen that is a key donor atom of a high-affinity ligand that scavenges and incapacitates redox-active iron that is the source of OH• generation. These novel reagents will be valuable tools to test hypotheses about the role of iron in neurodegeneration and may have therapeutic potential.
- Charkoudian, L. K.; Pham, D. M.; Franz, K. J. “A Pro-Chelator Triggered by Hydrogen Peroxide Inhibits Iron-Promoted Hydroxyl Radical Formation” J. Am. Chem. Soc.; 2006, 128, 12424–12425.
- Charkoudian, L. K.; Pham, D. M.; Kwan, A.; Vangeloff, A.; Franz, K. J., “Modifications of Boronic Ester Pro-chelators Triggered by Hydrogen Peroxide Tune Reactivity to Inhibit Metal-Promoted Oxidative Stress,” Dalton Trans., 2007, 43, 5031–5042. cover art
- Iron Prochelator BSIH Protects Retinal Pigment Epithelial Cells against Cell Death Induced by Hydrogen Peroxide, J. Inorg. Biochem., 2008, 102, 2130-2135.
- Dickens, M. G.; Franz, K. J., A Prochelator Activated by Hydrogen Peroxide Prevents Metal-Induced Amyloid beta Aggregation. ChemBioChem 2009, doi: 10.1002/cbic.200900597
- “A Prochelator Activated by β-Secretase Inhibits Aβ Aggregation and Suppresses Copper-Induced Reactive Oxygen Species Formation,” Folk, D. S.; Franz, K. J., J. Am. Chem. Soc. 2010, 132, 4994-4995. 10.1021/ja100943r
- Wang, Q.; Franz, K. J., The hydrolytic susceptibility of prochelator BSIH in aqueous solutions. Bioorg. Med. Chem. Lett. 2017, 27 (17), 4165-4170.
- Wang, Q.; Franz, K. J., Modifying aroylhydrazone prochelators for hydrolytic stability and improved cytoprotection against oxidative stress. Biorg. Med. Chem. 2018.
Caged Metal Complexes
In addition to protecting cells from oxidative stress, we are also interested in promoting such reactivity. For this strategy we are incorporating photolabile groups into the backbone of ligands to “cage” stable metal chelates. The inspiration for these compounds comes from the very successful caged calcium compounds that have found widespread use in studying Ca(II) in neurotransmission, muscle contraction, and other biological processes. We are interested in caging transition metal ions and taking advantage of their change in reactivity post-photolysis. Activation with UV light induces bond cleavage and release of metal complexes that are both more bioavailable and reactive toward ROS, as shown in cartoon form in the adjacent figure. These new reagents will be valuable tools for on-demand delivery of metal ions to study mechanisms of metal ion trafficking, as well as applications such as chemotherapy where toxic metal ions can be released to induce cell death.
- “A Photolabile Ligand for Light-Activated Release of Caged Copper,” Ciesienski, K. L.; Haas, K. L.; Dickens, M. G.; Tesema, Y. T. Franz, K. J. J. Am. Chem. Soc.
- “A Caged Platinum(II) Complex that Increases Cytotoxicity upon Light Activation,“ Ciesienski, K. L.; Hyman, L. M.; Yang, D. T.; Haas, K. L.; Dickens, M. G.; R. J. Holbrook; and Franz, K. J. Eur. J. Inorg. Chem. DOI: 10.1002/ejic.201000098
- “Toward the Detection of Cellular Copper(II) by a Light-Activated Fluorescence Increase,” Ciesienski, K. L.; Hyman, L. M.; Derisavifard, S.; Franz, K. J., Inorg. Chem. 2010, 49, 6808-6810.
Copper is required in virtually all cell types from bacteria to humans, and cells have elaborate systems for its uptake, transport, and distribution. In order to elucidate the molecular mechanisms by which cells acquire copper, we are investigating the copper binding interactions of peptide sequences found on the extracellular region of high-affinity copper transport proteins in the Ctr family. The unique feature of the extracellular region is the presence of methionine-rich domains arranged as MXXM or MXM motifs containing 3–5 methionine residues per “Mets” motif. We use peptide synthesis to create a series of MX2MX2M peptides and investigate their copper-binding properties by mass spectrometry, UV-vis spectroscopy and cyclic voltammetry. The Mets peptides bind selectively to Cu(I) with impressive affinity. Removing even one of the methionine residues abolishes this capability and establishes the MX2MX2M motif as a Cu(I) binding domain.
- Jiang, J.; Nadas, I. A.; Kim, M. A.; Franz, K. J. “A Mets Motif Peptide Found in Copper Transport Proteins Selectively Binds Cu(I) with Methionine-Only Coordination” Inorg. Chem., 2005, 44, 9787–9794.
- Crider, S. E.; Holbrook, R. J.; Franz, K. J. “Coordination of Platinum therapeutic Agents to Met-Rich Motifs of hCtr1” Metallomics, DOI: 10.1039/b916899k
- Rubino, J. T.; Riggs-Gelasco, P.; Franz, K. J. “Methionine motifs of copper transport proteins provide general and flexible thioether-only binding sites for Cu(I) and Ag(I)” J. Biol. Inorg. Chem., DOI: 10.1007/s00775-010-0663-9
Phosphorylation-Dependent Metal Binding
Proteins often bind metal ions by using the oxygen, nitrogen, or sulfur-containing functional groups of the amino acids that make up the protein. A protein’s complexity increases, however, by the action of enzymes that chemically modify specific amino acids, for example by adding a phosphate group. We are interested in understanding how protein phosphorylation changes a protein’s affinity for metal ions and how such phosphoprotein-metal complexes consequently alter protein conformation, as shown schematically below.
By using Tb3+ as a luminescent probe, we demonstrated that the phosphorylation state of a 14-residue peptide fragment of a-synuclein, a protein implicated in Parkinson’s disease, dramatically affects the metal ion affinity of the peptide. Whereas the unphosphorylated peptide and its phosphoserine analogue show weak Tb3+ binding, the phosphotyrosine analogue shows tight 1:1 binding as well as 2:1 and 3:1 Tb:peptide adducts. Our data suggest that the phosphorylated amino acid must be appropriately positioned among additional ligating residues to establish this phosphorylation-dependent metal binding.
- “Phosphorylation of an a-Synuclein Peptide Fragment Enhances Metal Binding” Liu, L. L.; Franz, K. J. J. Am. Chem. Soc. 2005, 127, 9662–9663.
- “Metal Binding Studies of Phosphorylated a-Synuclein Peptide Fragments” Liu, L. L.; Franz, K. J.; J. Biol. Inorg. Chem., 2007, 12, 234–247 .