Home » Urine Microscopy

Fellowship Program Leadership

Director-
Matthew A. Sparks, MD

Associate Director-
Harpreet Singh, MD

Associate Director-
Christina Wyatt, MD

Program Coordinator-
Marianne Drexler

Chief Fellow-
Rasha Raslan, MD

Urine Microscopy

Urine Microscopy Lecture by Dr. SparksView Page

Primer on how to spin urine (NephSIM)

Duke Hospital Urine Microscopy

The current chief fellow will ensure the microscope equipment is furnished on a monthly basis. Tracie Bach tracie.bach@duke.edu will order Urine Microscopy supplies.

we use Southern Microscopes to service their email is office@southernmicroscope.com  and phone number is 800-227-7276

code to the iPhone (VA and Duke) is 1234

Duke 1A Clinic Urine Microscope

for supplies let Gilda Maga (charge nurse know)

VA Urine Microscopy

For supplies contact Ykenia.Woods@va.gov and they will be delivered to the plexiglass specimen delivery window for pathology on 3rd floorr.

  • Urine Microscopy room: 4&5 together then 3.
  • The use of the urine microscopy room (centrifuge and microscope in room B3019) is restricted to the nephrology service, as this requires the nephrology providers (nephrology attendings and APPs) to receive appropriate training and to undergo quality control certification and frequent competency validations by the central lab. We have submitted a work order to have that room’s door code changed and will let you know what the new code is when that is completed.
  • The use of the urine microscopy room (centrifuge and microscope in room B3019) is restricted to the nephrology service, as this requires the nephrology providers (nephrology attendings and APPs) to receive appropriate training and to undergo quality control certification and frequent competency validations by the central lab.
  • Keep workstation clean after each use, and do not leave urine slides or sample on the station
  • Documentation
    • Enter the quality control verification and urine sediment microscopy results in CPRS note titled “MICROSCOPY  <PROVIDER PERFORMED MICROSCOPY RESULTS NOTE>”.  On a recent audit we found that this was not being consistently done.. It is important that this separate CPRS note be entered for every sample is inspected.  If the above CPRS note is entered by a nephrology trainee, then send the CPRS note to the attending for attestation and signature.
    • Quality Control:
      • We are working with the central lab on a quality control process that satisfies the Joint Commission and will let you know when finalized. In the meantime, please make sure to have a urinalysis order placed and sample sent to the central lab on the same day a urine sediment is done by you.

Duke 1A Microscope

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  • Download this app to your phone EP View either iPhone or Android
    • for problems with EP View app please call into our Technical Assistance Center at 800-225-8330, Option 4, then Option 1, and one of the Olympus support engineers will assist you. You must be near the microscope.
  • Go to your wifi setting and search for the camera hotspot network ****turn camera on (blue light should turn on)
    • Network should be EP50 and a series of numbers/letters
    • you will be prompted for password… it is 12345678
  • You can view slides at bright field (BF) or phase contrast (Ph)(turn the dial to BF or Ph)
    • for 10X  BF or Ph1
    • for 20X BF or Ph1
    • for 40X BF or Ph2
    • for 60X BF only
    • for 100x oil immersion BF and Ph3 (ONLY FOR Dr. Sparks to use)

To polarize a slide to look for crystals

put this polarizer on the light well (the red arrow on the image below)

U-POT Olympus Polarizer, rotate until the light is black and things that polarize (crystals) light up.