AAVs for CRISPR/Cas9 expression

  1. AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA Plasmid # 61591; Addgene- https://www.addgene.org/61591/
  2. BK- pAAV-EFS-NC- SpCas9-NLS-Poly(A)
  3. BK- pAAV-CMV-SpCas9- NLS-Poly(A)
  4. BK- pAAV-hSyn -SpCas9- NLS-Poly(A)
  5. BK- pAAV- -SpCas9- NLS-Poly(A)
  6. pAAV- nEF promoter- Cas9; Plasmid #87115; Addgene- http://www.addgene.org/87115/
  7. pAAV-pMecp2-SpCas9 Plasmid #60957 http://www.addgene.org/60957/
  8. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA Plasmid #61593; Addgene- http://www.addgene.org/61593/
  9. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Plasmid #61591; Addgene- http://www.addgene.org/61591/
  10. AAV-NFS-NC: NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
  11. AAV-Syn: NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
  12. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA; Plasmid #61592; Addgene- http://www.addgene.org/61592/
  13. pJEP12-AAV-H1/TO(dox-regulated)-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA; Plasmid #82704; Addgene- http://www.addgene.org/82704/
  14. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA; Plasmid #61594; Addgene-

http://www.addgene.org/61594/

  1. AAV-NFS-NC:NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
  2. AAV-Syn:NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
  3. AAV-MeCp2:NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
  4. AAV-NFS-NC:NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
  5. AAV-NFS-NC:NLS-dSaCas9Nickase-NLS-3xHA-bGHpA
  6. AAV-CMV:NLS-dSaCas9Nickase-NLS-3xHA-bGHpA
  7. AAV-CAG:NLS-dSaCas9Nickase NLS-3xHA-bGHpA
  8. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR; Plasmid #60231; Addgene- http://www.addgene.org/60231/
  9. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR; Plasmid #60226; Addgene-

http://www.addgene.org/60226/

  1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL); Plasmid #60224; http://www.addgene.org/60224/
  2. BK- pAAV-TRE – SpCas9-NLS-Poly(A)
  3. BK- pAAV-TRE- SaCas9-NLS-Poly(A)
  4. BK- pAAV-TRE – dSpCas9-NLS-Poly(A)
  5. BK- pAAV-TRE – dSaCas9-NLS-Poly(A)
  6. BK- pAAV-TRE – SpCas9Nickase-NLS-Poly(A)
  7. BK- pAAV-TRE – SaCas9Nickase-NLS-Poly(A)