Lentiviral vector for CRISPR/Cas9 expression

Lentiviral vectors- backbones

  1. pBK109- short version derived from pLentiCRISPR/v2 ready-for-gRNA-cloning (BsmBI)
  2. pBK301- short version of pLentiCRISPR/v2 with 2 Sp1 binding sites upstream of U6p- gRNA cloning site BsmBI
  3. pBK175- short version of pLentiCRISPR/v2 with 4 Sp1 binding sites upstream of U6p- gRNA cloning site BsmBI
  4. pBK110- pLentiCRISPR/v2 Luciferase-gRNA
  5. pBK104- pLentiCRISPR/v2 GABA a2-subunit-gRNA
  6. pBK83- pLentiCRISPR/v2 HDAC1-1 –gRNA
  7. pBK84-pLentiCRISPR/v2 HDAC1-2 –gRNA
  8. pBK85-pLentiCRISPR/v2 HDAC1-3 – gRNA
  9. pBK86-pLentiCRISPR/v2 GFP1 – gRNA
  10. pBK87-pLentiCRISPR/v2 GFP2 – gRNA
  11. pBK88-pLentiCRISPR/v2 GFP3 – gRNA
  12. pBK189- pLentiCRISPR/v2 with GFP-gRNA
  13. pBK198- short version of pLentiCRISPR/v2 with 2 Sp1 binding sites with GFP-gRNA
  14. pBK180- rtTA3 Blasticidin third generation rtTA3 can be used for tet-Cas9 system (next plasmid)
  15. pBK185- tet- inducible Cas9- all-in-one short- with BsmBI for cloning sgRNA
  16. pBK195- tet- inducible Cas9- all-in-one short- with GFP-gRNA
  17. pCW-Cas9 Tet ON Plasmid #50661; Addgene- https://www.addgene.org/50661/
  18. pBK109easy -GFP pLenti-SpCas9gRNA with GFP all-in-one
  19. pBK97- SpCas9 nickase- all-in-one lentiviral backbone; titer-optimized backbone; ready for gRNA cloning BsmBI
  20. pBK456- dCas9- all-in-one lentiviral backbone; titer-optimized backbone; ready for gRNA cloning BsmBI
  21. pBK109easy -GFP pLenti-SpCas9gRNA with GFP all-in-one
  22. pBK109BL- short version of pLentiCRISPR/v2 gRNA cloning site BsmBI with blasticidin
  23. pBK114- pLenti-Cas9-GFP
  24. pLentiCRISPR/v2 Plasmid #52961; Addgene- https://www.addgene.org/52961/
  25. pLenti-multi-CRISPR; Plasmid #85402; Addgene- https://www.addgene.org/85402/
  26. LentiCRISPRv2-mCherry; Plasmid # 99154; Addgene- http://www.addgene.org/99154/
  27. pLenti-pU6-sgRNA Ef1alpha-Puro-T2A-BFP Plasmid #84832; Addgene- http://www.addgene.org/84832/
  28. pLenti-sgRNA; Plasmid #71409; Addgene- http://www.addgene.org/71409/
  29. pLenti-CRISPR.EFS.tRFP; Plasmid #57819; Addgene- http://www.addgene.org/57819/
  30. lentiCas9-Venus; Plasmid # 70267; Addgene- http://www.addgene.org/70267/
  31. pL-CRISPR.EFS.GFP Plasmid #57818; Addgene- https://www.addgene.org/57818/
  32. lentiCas9-Blast Plasmid #52962; Addgene- https://www.addgene.org/52962/
  33. lentiCas9-Puro Plasmid #52963; Addgene-http://www.addgene.org/52963/
  34. lentiCas9-EGFP; Plasmid #63592; Addgene- https://www.addgene.org/63592/
  35. lentiCas9n(D10A)-Blast; Plasmid #63593; Addgene- http://www.addgene.org/63593
  36. lenti-dCAS-VP64-Blast; Plasmid #61425; Addgene- http://www.addgene.org/61425/
  37. pHAGE TRE dCas9-VP64; Plasmid #50916; Addgene- http://www.addgene.org/50916/
  38. lenti-TRE-KRAB-dCas9-IRES-BFP; Plasmid #85449; Addgene- http://www.addgene.org/85449/
  39. plenti- hUbC-dCas9 VP64-T2A-GFP; Plasmid #53192; Addgene- http://www.addgene.org/53192/
  40. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP; Plasmid #50946; Addgene- http://www.addgene.org/50946/
  41. plentiSAMv2; Plasmid #75112; Addgene- http://www.addgene.org/75112/
  42. pHR-SFFV-KRAB-dCas9-P2A-mCherry; Plasmid #60954; Addgene- http://www.addgene.org/60954/
  43. pLV-dCas9-KRAB-PGK-Hyg- Plasmid #83890; Addgene- http://www.addgene.org/83890/
  45. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP; Plasmid #71237; Addgene- http://www.addgene.org/71237/
  46. pHAGE EF1α dCas9-KRAB; Plasmid #50919; Addgene- http://www.addgene.org/50919/
  47. lenti sgRNA-MS2-Zeo; Plasmid #61427; Addgene- http://www.addgene.org/61427/
  48. lenti sgRNAMS2- puro optimized backbone; Plasmid #73797; Addgene- http://www.addgene.org/73797/
  49. pHAGE-TO-nmdCas9-3XGFP; Plasmid #64109; Addgene- http://www.addgene.org/64109/
  50. pLV-dCas9-p300-P2A-Puro; Plasmid #83889; Addgene- http://www.addgene.org/83889/
  51. Lenti_sgRNA-EFS-GFP; Plasmid #65656; Addgene- http://www.addgene.org/65656/
  52. Lenti-AsCpf1-Blast; Plasmid #84750; Addgene- http://www.addgene.org/84750/
  53. LentiCRISPRv2Cre; Plasmid #82415;  Addgene- http://www.addgene.org/82415/
  54. Exp_v-pcDNA3.1-hAsCpf1; Plasmid #69982; Addgene- https://www.addgene.org/69982/
  55. Exp_v-pSimpleII-U6- gRNA cloning site BsmBI-NLS-NmCas9-HA-NLS plasmid # 47868; Addgene https://www.addgene.org/47868/
  56. Exp_v-pSaCas9_GFP; Plasmid # 64709; Addgene- https://www.addgene.org/64709/
  57. Exp_v-Csy4-T2A-Cas9-NLS; Plasmid # 53371; Addgene- https://www.addgene.org/53371/
  58. Exp_v-ppcDNA3.1-hFnCpf1; Plasmid #69976; Addgene- https://www.addgene.org/69976/
  59. Exp_v-eSpCas9(1.1)- Plasmid #71814; Addgene-https://www.addgene.org/71814/
  60. Exp_v-SpCas9-HF1 (high fidelity Cas9); Plasmid #72247; Addgene- https://www.addgene.org/72247/