Collection-AAV

ADENO-ASSOCIATED VECTORS:

Duke University Viral Vector Core plasmids: Control optogenetic and other constructs (no MTA required, unless specified otherwise). Publications should acknowledge the donor of the reagent and the Viral Vector Core at Duke University.

 

Reporter constructs & cassettes for cloning

  1. AAV-CAG-eGFP
  2. AAV-EF1 alpha-eGFP
  3. AAV-EFS-NC-eGFP
  4. AAV-CMV-eGFP
  5. AAV-CAG empty cassette
  6. AAV-CMV-eGFP-CRE
  7. AAV-CaMKIIa- eGFP
  8. AAV- hSyn-eGFP
  9. pAAV-GFAP104-mCherry (Addgene #58909)

 Note: This is just a small portion of AAV-vector backbones available from our core facility; the core recently developed a collection of empty, GFP/cherry, p2A, t2A, IRES vectors ready-for cloning of bicistronic transgenes with different promoters – email to viral.core@duke.edu or directly to the VVC director:  boris.kantor@duke.edu to inquire about our collection. Please note: all vector backbones are optimized and checked for packaging and expression.

 

Dr. Karl Deisseroth AAV lab constructs

For first time order of these vectors you will have to fill an MTA form with Dr. Deisseroth. To obtain MTA, please please contact Dr. Karl Deisseroth and Charu Ramakrishnan. Please upload a copy of your MTA when placing your online order.

Contact email of Charu Ramakrishnan: charur@stanford.edu

Contact email of Dr. Karl Deisseroth: deissero@stanford.edu

 

Control optogenetic constructs:

  1. AAV-EF1a-DIO-eYFP
  2. AAV-EF1a-DIO-mCherry
  3. AAV-CaMKIIa- eYFP
  4. AAV-CaMKIIa- mCherry
  5. AAV- hSyn- eYFP
  6. AAV- hSyn- mCherry

 

Optical Excitation: Volvox Channelrhodopsin-1 (VChR1)

Available in a standard mammalian expression vector and lentivirus vectors

  1. 1/VChR1-EYFP
  2. 1/VChR1-mCherry

For more information see:

A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins.

Color-tuned channelrhodopsins for multiwavelength optogenetics.

 

Maps of the constructs are available here http://web.stanford.edu/group/dlab/optogenetics/sequence_info.html

 

 

*The VChR1 gene is codon-optimized for mammalian expression.

Optical Inhibition: Halorhodopsin (NpHR)

For more info see: Amygdala circuitry mediating reversible and bidirectional control of anxiety.

 

Second (2.0) and Third (3.0) generations of NpHR are available. eNpHR2.0 may be superior in some cell types, including photoreceptors, see: Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa.

 

  1. AAV-EF1a-DIO-eNpHR 3.0-eYFP
  2. AAV-CaMKIIa- eNpHR 3.0-eYFP
  3. AAV-hSyn- eNpHR 3.0-eYFP
  4. AAV-EF1a-DIO- eNpHR 3.0- mCherry
  5. AAV- CaMKIIa- eNpHR 3.0- mCherry
  6. AAV- hSyn- eNpHR 3.0- mCherry
  7. AAV- hThy-1- eNpHR 3.0- eYFP
  8. AAV-DIO-eNpHR-eYFP *(no need MTA)

 

Optical Excitation: Channelrhodopsin-2 (ChR2)

For more info see:

Channelrhodopsin-2 and optical control of excitable cells.

Circuit-breakers: optical technologies for probing neural signals and systems.

 

  1. AAV-EF1a-DIO-hCHR2(H134R)-mCherry
  2. AAV-EF1a-DIO-hCHR2(H134R)-EYFP
  3. AAV-CaMKIIa- hCHR2(H134R)-EYFP
  4. AAV-CaMKIIa- hCHR2(H134R)-mCherry
  5. AAV-hSyn- hCHR2(H134R)-EYFP
  6. AAV-hSyn- hCHR2(H134R)-mCherry
  7. AAV-CaMKIIa- hCHR2(C128S/D156A)-EYFP
  8. AAV-CaMKIIa- hCHR2(C128S/D156A)-mCherry
  9. AAV-EF1a-DIO- hCHR2(C128S/D156A)-EYFP
  10. AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry
  11. AAV- CaMKIIa – hCHR2(et/tc)-EYFP
  12. AAV- CaMKIIa – hCHR2(et/tc)-mCherry
  13. AAV-CaMKIIa- hCHR2(E123A)-EYFP
  14. AAV-CaMKIIa- hCHR2(E123A)-mCherry
  15. AAV-EF1a-DIO- hCHR2(et/tc)-EYFP
  16. AAV-EF1a-DIO- hCHR2(et/tc)-mCherry
  17. AAV-EF1a-DIO- hCHR2(E123A)-EYFP
  18. AAV-EF1a-DIO- hCHR2(E123A)-mCherry
  19. AAV- hSyn- hCHR2(E123A)-EYFP
  20. AAV-GFAP-DIO-hCHR2(H134R)-EYFP
  21. AAV-GFAP-DIO-hCHR2(H134R)-mCherry
  22. AAV-EF1a-DIO- hCHR2(T159C)- EYFP
  23. AAV-EF1a-DIO- hCHR2(T159C)- mCherry
  24. AAV- CaMKIIa- C1V1 (E162T)- p2A- EYFP
  25. AAV-CaMKIIa- hCHR2(T159C)- p2A- EYFP
  26. AAV-CaMKIIa- hCHR2(T159C)- p2A- mCherry
  27. AAV-CaMKIIa- hCHR2(et/tc)- p2A- EYFP
  28. AAV-CaMKIIa- hCHR2(et/tc)- p2A- mCherry
  29. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- EYFP
  30. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry

 

Cre-inducible DIO (double-floxed inverse ORF) AAV

For more info see: Glutamatergic neurotransmission between the C1 neurons and the parasympathetic preganglionic neurons of the dorsal motor nucleus of the vagus.

 

  1. AAV-EF1a-DIO-mCherry
  2. AAV-EF1a-DIO-hCHR2(H134R)-mCherry
  3. AAV-EF1a-DIO-hCHR2(H134R)-EYFP
  4. AAV-EF1a-DIO-eNpHR 3.0-EYFP
  5. AAV-EF1a-DIO-chETA-eYFP
  6. AAV-EF1a-DIO-EYFP
  7. AAV-EF1a-DIO- C1V1 (t/t)-TS- eYFP
  8. AAV-EF1a-DIO- C1V1 (t/t)-TS- mCherry
  9. AAV-EF1a-DIO- hCHR2(C128S/D156A)-eYFP
  10. AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry
  11. AAV-EF1a-DIO- hCHR2(et/tc)-eYFP
  12. AAV-EF1a-DIO- hCHR2(et/tc)-mCherry
  13. AAV-EF1a-DIO- hCHR2(E123A)-eYFP
  14. AAV-EF1a-DIO- hCHR2(E123A)-mCherry
  15. AAV-EF1a-DIO- eNpHR 3.0- mCherry
  16. AAV-GFAP-DIO-hCHR2(H134R)-eYFP
  17. AAV-GFAP-DIO-hCHR2(H134R)-mCherry
  18. AAV-EF1a-DIO- eArch3.0- eYFP
  19. AAV-EF1a-DIO- hCHR2(T159C)- eYFP
  20. AAV-EF1a-DIO- hCHR2(T159C)- mCherry
  21. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- eYFP
  22. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry
  23. AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- eYFP
  24. AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- mCherry
  25. AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- eYFP
  26. AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry

 

Bi-stable excitation: Step Function Opsins (SFOs)

For more info see: Bi-stable neural state switches.

 

  1. AAV-CaMKIIa- hCHR2(C128S/D156A)-eYFP
  2. AAV-CaMKIIa- hCHR2(C128S/D156A)-mCherry
  3. AAV-EF1a-DIO- hCHR2(C128S/D156A)-eYFP
  4. AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry

 

Optical Control of Intracellular Signaling: Opto-XRs

For more info see: Color-tuned channelrhodopsins for multiwavelength optogenetics.

Coming soon- please check for availability

  1. AAV-CaMKII-opto A1-eYFP
  2. AAV-CaMKIIa-Opto A1-eYFP
  3. pAAV-CaMKIIa-Opto B2-eYFP
  4. AAV-CaMKIIa-Opto D1-eYFP
  5. AAV-EF1a-DIO Opto A1-eYFP
  6. AAV-EF1a-DIO Opto A1-mCherry
  7. AAV-EF1a-DIO Opto B2-eYFP
  8. AAV-EF1a-DIO Opto B2-mCherry
  9. AAV-EF1a-DIO Opto D1-eYFP
  10. AAV-EF1a-DIO Opto D1-mCherry
  11. AAV-hSyn Opto A1-eYFP
  12. AAV-hSyn Opto B2-eYFP
  13. AAV-hSyn Opto D1-eYFP

 

Ultrafast Optogenetic Control: ChETA

For more info see: Ultrafast optogenetic control.

 

  1. AAV-EF1a-DIO-chETA-eYFP

 

Third-generation Optogenetic Inhibition: Arch 3.0 ArchT 3.0, and Mac 3.0

For more info see: Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.

 

  1. AAV-EF1a-DIO-eArch 3.0-eYFP
  2. AAV-Ef1a-DIO eArch 3.0-eYFP
  3. AAV-CaMKIIa-eArchT 3.0-eYFP
  4. AAV-CaMKIIa-eArch 3.0-eYFP
  5. AAV-hThy1-eArch 3.0-eYFP
  6. AAV-hSyn-eArch 3.0-eYFP

Trans-synaptic Tracers: WGA-Cre

Trans-synaptic tracing tools are capable to assist in targeting neuronal subpopulations based on their synaptic connectivity to a downstream region. These may be used in conjunction with any of the DIO class of targeting virus. Researchers need to determine the directionality and extent of spread of the WGA-cre translocation in each experimental system.

  1. AAV-EF1a-mCherry-IRES-WGA-Cre

Second-generation Ultrafast Optogenetics

  1. AAV-CaMKIIa- hCHR2(H134R)-eYFP
  2. AAV-CaMKIIa- hCHR2(H134R)-mCherry
  3. AAV-hSyn- hCHR2(H134R)-eYFP
  4. AAV-hSyn- hCHR2(H134R)-mCherry
  5. AAV-CaMKIIa- hCHR2(C128S/D156A)-eYFP
  6. AAV-CaMKIIa- hCHR2(C128S/D156A)-mCherry
  7. AAV-EF1a-DIO- hCHR2(C128S/D156A)-eYFP
  8. AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry
  9. AAV- CaMKIIa – hCHR2(et/tc)-eYFP
  10. AAV- CaMKIIa – hCHR2(et/tc)-mCherry
  11. AAV-CaMKIIa- hCHR2(E123A)-eYFP
  12. AAV-CaMKIIa- hCHR2(E123A)-mCherry
  13. AAV-EF1a-DIO- hCHR2(et/tc)-eYFP
  14. AAV-EF1a-DIO- hCHR2(et/tc)-mCherry
  15. AAV-EF1a-DIO- hCHR2(E123A)-eYFP
  16. AAV-EF1a-DIO- hCHR2(E123A)-mCherry
  17. AAV- hSyn- hCHR2(E123A)-eYFP
  18. AAV- hSyn- eYFP
  19. AAV- hSyn- mCherry
  20. AAV-EF1a-DIO- hCHR2(T159C)- eYFP
  21. AAV-EF1a-DIO- hCHR2(T159C)- mCherry
  22. AAV-CaMKIIa- hCHR2(T159C)- p2A- eYFP
  23. AAV-CaMKIIa- hCHR2(T159C)- p2A- mCherry
  24. AAV-CaMKIIa- hCHR2(et/tc)- p2A- eYFP
  25. AAV-CaMKIIa- hCHR2(et/tc)- p2A- mCherry
  26. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- eYFP
  27. AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry

Stabilized Step Function Opsins SSFO

Newly developed ChR2 variants which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. They can be deactivated by yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.

  1. AAV-EF1a-SSFO- eYFP (coming soon-check availability)
  2. AAV-hSyn-SSFO-eYFP (coming soon-check availability)
  3. AAV-CaMKIIa-hChR2 (C128S/D156A)-eYFP
  4. AAV-CaMKIIa- hChR2 (C128S/D156A)-mCherry
  5. AAV-Ef1a-DIO hChR2 (C128S/D156A)-eYFP
  6. AAV-Ef1a-DIO hChR2 (C128S/D156A)-mCherry

 

Red-Shifted Optical Excitation: C1V1 variants C1V1

For more info see: Two-photon optogenetics of dendritic spines and neural circuits.

Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields.

 

  1. AAV-CaMKIIa- C1V1 (t/t)-TS- eYFP
  2. AAV-CaMKIIa- C1V1 (t/t)-TS- mCherry
  3. AAV-EF1a-DIO- C1V1 (t/t)-TS- eYFP
  4. AAV-EF1a-DIO- C1V1 (t/t)-TS- mCherry
  5. AAV- CaMKIIa- C1V1 (E162T)- p2A- eYFP
  6. AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- eYFP
  7. AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- mCherry
  8. AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- eYFP
  9. AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- mCherry
  10. AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- eYFP
  11. AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- mCherry
  12. AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- eYFP
  13. AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry

 

Cell-filling variants

Bi-cistronic expression of opsin and fluorophore allows for enhanced identification of opsin-expressing cells

  1. AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry
  2. AAV-CamKII-hChR2 (T159C)-p2A-eYFP-WPRE
  3. AAV-CamKII-hChR2 (T159C)-p2A-mCherry-WPRE
  4. AAV-CamKII-hChR2 (E123T/T159C)-p2A-eYFP-WPRE
  5. AAV-CamKII-hChR2 (E123T/T159C)-p2A-mCherry-WPRE
  6. AAV-CamKIIa-C1V1 (E162T)-TS-p2A-eYFP-WPRE
  7. AAV-CamKIIa-C1V1 (E162T)-TS-p2A-mCherry-WPRE
  8. AAV-CamKIIa-C1V1 (t/t)-TS-p2A-eYFP-WPRE
  9. AAV-CamKIIa-C1V1 (t/t)-TS-p2A-mCherry-WPRE
  10. AAV-Ef1a-DIO hChR2 (E123T/T159C)-p2A-eYFP-WPRE
  11. AAV-Ef1a-DIO hChR2 (E123T/T159C)-p2A-mCherry-WPRE
  12. AAV-Ef1a-DIO C1V1 (E162T)-TS-p2A-eYFP-WPRE
  13. AAV-Ef1a-DIO C1V1 (E162T)-TS-p2A-mCherry-WPRE
  14. AAV-Ef1a-DIO C1V1 (t/t)-TS-p2A-eYFP-WPRE
  15. AAV-Ef1a-DIO C1V1 (t/t)-TS-p2A-mCherry-WPRE
  16. AAV-CamKIIa-hChR2(C128A/H134R)-p2A-eYFP
  17. AAV-Ef1a-DIO hChR2(C128A/H134R)-p2A-eYFP

 

SwiChR and iC1C2: Action potential inhibition with chloride-conducting channelrhodopsins

Step-Waveform Inhibitory ChannelRhodopsin (SwiChR) variant is activated by brief blue light stimulation at low intensities, remains open in the dark for an extended period of time and gets deactivated by red light. The engineered iC1C2 was designed based on the 2012 crystal structure of C1C2 to conduct chloride ions instead of cations, utilizing physiological chloride gradients to precisely inhibit action potentials in response to blue light.

Coming soon-check for availability

  1. AAV-CaMKIIa-iC1C2 2.0-ts-eYFP
  2. AAV-CaMKIIa-SwiChRCA-ts-eYFP
  3. AAV-EF1a-DIO iC1C2 2.0-ts-eYFP-WPRE
  4. AAV-EF1a-DIO SwiChRCA-ts-eYFP-WPRE

 

INTRSECT (INTronic Recombinase Sites Enabling Combinatorial Targeting)-related constructs

For more info see: Targeting cells with single vectors using multiple-feature Boolean logic.

Coming soon-check for availability

  1. AAV-EF1a-DIO-eYFP
  2. AAV-Ef1a-Cre-WPRE
  3. AAV-Ef1a-Dre-WPRE
  4. AAV-nEF-Con/Fon hChR2(H134R)-eYFP-WPRE
  5. AAV-nEF-Con/Foff hChR2(H134R)-eYFP-WPRE
  6. AAV-nEF-Coff/Fon hChR2(H134R)-eYFP-WPRE
  7. AAV-hSyn-Con/Fon eYFP-WPRE
  8. AAV-hSyn-Con/Fon hChR2(H134R)-eYFP-WPRE
  9. AAV-hSyn-Con/Foff hChR2(H134R)-eYFP-WPRE
  10. AAV-hSyn-Con/Foff eYFP-WPRE
  11. AAV-hSyn-Coff/Fon eYFP-WPRE
  12. AAV-hSyn-Coff/Fon hChR2(H134R)-eYFP-WRE
  13. AAV-Ef1a-vCre-WPRE
  14. AAV-EF1a-vCreDIO hChR2(H134R)-eYFP-WPRE
  15. AAV-Ef1a-sCre-WPRE
  16. AAV-EF1a-sCreDIO hChR2(H134R)-eYFP-WPRE
  17. AAV-EF1a-mCherry-IRES-Flpo-WPRE
  18. AAV-EF1a-mCherry-IRES-Cre-WPRE
  19. AAV-EF1a-mCherry-IRES-Dre-WPRE
  20. AAV-EF1a-mCherry-IRES-sCre-WPRE
  21. AAV-EF1a-mCherry-IRES-vCre-WPRE
  22. AAV-Ef1a-Flpo-WPRE
  23. AAV-Ef1a-fDIO-eYFP-WPRE
  24. AAV-EF1a-fDIO-hChR2(H134R)-eYFP-WPRE
  25. AAV-Ef1a-dDIO hChR2(H134R)-eYFP-WPRE

There is no MTA requirement for the below reagents, unless used for commercial purposes. Publications should acknowledge the donor of the reagent and the Duke University Viral Vector Core.

 

Dr. Adam Cohen lab constructs

DRH337: AAV-hsyn-CheRiff-eGFPArchaerhodopsin-based voltage indicators; Channelrhodopsin actuator

For more info see: All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.

 

  1. AAV-hSyn-CheRiff-eGFP #51697 Addgene
  2. AAV-CaMKIIa-QuasAr2-mO2 #51696 Addgene

 

Dr. Naoshige Uchida lab constructs

 

  1. AAV-CA-FLEX-RG Insert: Rabies virus glycoprotein rabies virus #38043 Addgene
  2. AAV-mCherry-flex-dtA   Insert: diphtheria toxin DIP_RS18250 #58536 Addgene
  3. AAV-CA-FLEX #38042 Addgene
  4. AAV-EF1a-FLEX-TVA-mCherry Insert: Avian sarcoma and leukemia virus receptor 950 38044

#38044 Addgene

 

 

Dr. Bryan Roth lab constructs

Designer receptors exclusively activated by designer drugs (DREADDs)

For more info see: DREADDs: novel tools for drug discovery and development.

 

Control vectors frequently ordered with DREADDs

pAAV-GFAP-EGFP (Plasmid #50473)

pAAV hSyn GFP

pAAV hSyn-EYFP

pAAV CMV GFP

pAAV CBa GFP

pAAV hSyn mCherry

pAAV EF1- alpha-DIO-EYFP

pAAV EF1- alpha-DIO-GFP

pAAV EF1- alpha-DIO-mCherry

pAAV EF1- alpha-mCherry

pAAV CamKII-EYFP

pAAV CamKII-mCherry

pAAV EF1- alpha-mCherry

pAAV GFAP-mCherry

 

DREADD-constructs

  1. pAAV-hSyn-HA-hM3D(Gq)-mCherry (Plasmid #50474)
  2. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine (Plasmid #50455)
  3.  pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (Plasmid #50467)
  4.  pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Plasmid #44361)
  5.  pAAV-CaMKIIa-hM3D(Gq)-mCherry (Plasmid #50476)
  6.  pAAV-CaMKIIa-HA-KORD-IRES-mCitrine (Plasmid #65418)
  7.  pAAV-hSyn-DIO-EGFP (Plasmid #50457)
  8.  pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine (Plasmid #50464)
  9.  pAAV-GFAP-hM4D(Gi)-mCherry (Plasmid #50479)
  10.  pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine (Plasmid #50471)
  11.  pAAV-hSyn-HA-hM4D(Gi)-mCherry (Plasmid #50475)
  12.  pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (Plasmid #65417)
  13.  pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine (Plasmid #50463)
  14.  pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine (Plasmid #50456)
  15.  pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine (Plasmid #50470)
  16.  pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine (Plasmid #50468)
  17.  pAAV-hSyn-DIO-hM4D(Gi)-mCherry (Plasmid #44362)
  18.  pAAV-hSyn-DIO-rM3D(Gs)-mCherry (Plasmid #50458)
  19.  pAAV-CaMKIIa-hM4D(Gi)-mCherry (Plasmid #50477)
  20.  pAAV-EF1a-DIO-hM3D(Gq)-mCherry (Plasmid #50460)
  21.  pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (Plasmid #50472)
  22.  pAAV-EF1a-DIO-hM4D(Gi)-mCherry (Plasmid #50461)
  23.  pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (Plasmid #50454)
  24.  pAAV-GFAP-hM3D(Gq)-mCherry (Plasmid #50478)
  25.  pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine (Plasmid #50466)

 

Dr. Edward Boyden lab constructs

Neural Silencers/Activators

For more info see: Millisecond-timescale, genetically targeted optical control of neural activity.

Prosthetic systems for therapeutic optical activation and silencing of genetically-targeted neurons.

A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane .

 

  1. AAV-hSyn-Arch-GFP
  2. AAV-FLEX-Arch-GFP
  3. AAV-FLEX-GFP
  4. AAV-CAG-ArchT-GFP
  5. AAV-CAG-ChR2-GFP
  6. AAV-FLEX-ArchT-tdTomato
  7. AAV-FLEX-tdTomato
  8. AAV-FLEX-ArchT-GFP
  9. AAV-CAG-ArchT-tdTomato
  10. AAV-FCK-ArchT-GFP
  11. AAV-CAG-GFP
  12. AAV-Syn-ChrimsonR-tdT
  13. AAV-CamKII-ArchT-GFP
  14. AAV-CAG-Arch-GFP
  15. AAV-CAG-FLEX-Mac-GFP
  16. AAV-CaMKII-Chronos-GFP
  17. AAV-Syn-ChrimsonR-GFP
  18. AAV-Syn-CsChR-GFP
  19. AAV-CaMKII-Mac-GFP
  20. AAV-EF1a-FLEX-ArchT-GFP
  21. AAV-Syn-FLEX-Mac-GFP
  22. AAV-Syn-Mac-GFP
  23. AAV-Syn-GFP
  24. AAV-Syn-ChR2(H134R)-GFP
  25. AAV-Syn-ChR2-GFP
  26. AAV-GFAP104-ChR2-mCherry
  27. AAV-GFAP104-mCherry
  28. pAAV-Syn-CoChR-GFPAAV-Syn-CoChR-GFP
  29. AAV-Syn-Chronos-GFP
  30. AAV-CAG-tdTomato (codon diversified)

 

Dr. Roger Tsien lab constructs

Red-shifted variant of channelrhodopsin and chromophore-assisted light inactivation

For more info see:

ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation.

Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI).

Characterization of engineered channelrhodopsin variants with improved properties and kinetics.

 

  1. AAV- ReaChR-citrine
  2. AAV-flex-ReaChR-citrine
  3. AAV-miniSOG-VAMP2-citrine
  4. AAV2-miniSOG-VAMP2-T2A-mCherry
  5. AAV-SYP1-miniSOG-citrine
  6. AAV-SYP1-miniSOG-T2A-mCherry
  7. AAV-flex-oChIEF-citrine
  8. AAV-oChIEF-citrine
  9. AAV-oChEF-citrine
  10. AAV-oChD-citrine
  11. AAV-oChIEF-tdTomato

Dr. Ian Wickersham lab constructs

Mono-synaptic sensitive tracers

For more info see: Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits.

 

  1. AAV- CAG-FLEX-splitTVA800
  2. AAV-synP-FLEX-EGFP-B19G   *Alternative name: pAAV-synP-FLEX-EpB
  3. AAV-CAG-FLEX-splitTVA950
  4. AAV-CAG-FLEX-EGFP
  5. AAV-CAG-FLEX-splitTVA-EGFP *Alternative name: pAAV-CAG-FLEX-sTpE
  6. AAV-CAG-FLEX-TVA66T-EGFP
  7. AAV-synP-FLEX-TVA66T-EGFP
  8. AAV-synP-FLEX-splitTVA-EGFP *Alternative plasmid name: pAAV-synP-FLEX-sTpE

 

Dr. Baljit Khakh lab constructs

  1. 1 gfaABC1D-Lck-GCaMP3
  2. 1 gfaABC1D-Cyto-GCaMP3
  3. 1 gfaABC1D-tdTomato
  4. 1 gfaABC1D-EGFP-Kir4.1
  5. 1 gfaABC1D-lck-GCaMP6f
  6. 1 gfaABC1D-cyto-GCaMP6f

 

Dr. Jonathan Ting lab construct

283.  AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

284.  AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

285.  AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

286. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

287. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

288. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

289. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

290. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

291. pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2

292. pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFP

293. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

 

CRE-expressing vectors

294. AAV-CAG- GFP/Cre (Plasmid #49056)

295. AAV-CMV-GFP/CRE

296. AAV-CamKII-GFP/CRE

297. AAV-Syn-GFP/CRE

298.  AAV-NFS-NC-GFP/CRE

299. AAV-EF1Alpha-GFP/CRE

300. AAV pmSyn1-EBFP-Cre (Plasmid #51507)

301. AAV-Cre-GFP (Plasmid #68544)

302. AAV-CAG-CRE-GFP

303. AAV-CMV-CRE-GFP

304. AAV-Syn-CRE-GFP

305- AAV-CamKII-CRE-GFP

306. AAV-pgk-Cre (Plasmid #24593)

307. pAAV-EF1a-C-CreintG (Plasmid #69571)

308. pAAV-Ef1a-mCherry-IRES-Cre (Plasmid #55632)

309. AAV-EFS-NC-Cherry-CRE

310. AAV-Syn-Cherry-CRE

311. pAAV-EF1a-Cre (Plasmid #55636)

312. AAV-CREB (Plasmid #68550)

313. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (Plasmid #87306)

314. AAV-TRE-Cre-WPRE (Supernova) (Plasmid #85040)

315. AAV-TRE-GFP-CRE

316. pAAV.cTNT.iCre (Plasmid #69916)

317. paavCAG-iCre (Plasmid #51904)