Retroviral vectors- grades/volumes

We offer retroviral vector particles in the following formats

  • Small Non-Concentrated Retrovirus Preps (volume-10mL, at the concentration 5 x106 vg/mL)

Production time: 7 days

 

  • Large Non-Concentrated Retrovirus Preps (volume-100mL, at the concentration ≥ 5 x106 vg/mL). Vectors will be supplied in the aliquots of 10 mL

 

Production time: 7 days.

 

  • Concentrated Retrovirus In-Vitro Grade Preps (volume-100uL, at the concentration ≥ 1 x109 vg/mL).

Note: this grade is not suitable for in-vivo use. Vectors will be supplied in the aliquots of 20 uL. Production time: 2 weeks.

 

 

  • Small Concentrated Retrovirus In-Vivo Grade Preps (volume-25uL, at the concentration ≥ 1 x109 vg/mL).
  • This vector grade greatly fits small in-vivo pilot experiments. Vectors will be supplied in the aliquots of 5 uL.

Note: The cost includes testing for appearance of Replication Competent Retrovirus (RCR) – following FDA recommendations in “Guidance for Industry—Supplemental Guidance on Testing for Replication Competent Retrovirus in Retroviral Vector Based Gene Therapy Products and During Follow-up of Patients in Clinical Trials Using Retroviral Vectors” (Gunter et al. 1993) and the Vaccines and Related Biological Products Advisory Committee (October 25, 1993). Production time: 2 weeks.

 

 

  • Medium (Standard) Concentrated Retrovirus In-Vivo Grade Preps (volume-150uL, at the concentration ≥ 1 x109 vg/mL).

The cost includes testing for appearance of Replication Competent Retrovirus (RCR)

Production time: 2 weeks.

 

 

  • Large Concentrated Retrovirus In-Vivo Grade Preps (volume-450uL, at the concentration ≥ 2.5 x109 vg/mL).

Vectors of this grade will be suitable for large-scale in vivo studies. Vectors will be supplied in the aliquots of 25 uL.

The cost includes testing for appearance of Replication Competent Retrovirus (RCR)

Production time: 3 weeks

 

GRADE TABLE

Prep Vector Titer/Quantity  

 

   Time of Production
Small Non-Concentrated for In Vitro Use ≥5 x106 vg/mL, total 10mL    7days
Large Non-Concentrated for In Vitro Use ≥5 x106 vg/mL, total 100mL    7days
Concentrated for In Vitro Use ≥1 x109 vg/mL, total 150uL  2 weeks
Small Concentrated for In Vivo Use ≥1 x109 vg/mL, total 25uL   2weeks
Medium (Standard) Prep for In Vivo Use ≥1 x109 vg/mL, total 150uL   2weeks
Large Concentrated for In Vivo Use ≥2.5 x109 vg/mL, total 450uL  

 

   3weeks