AAV-grades/volumes

We offer adeno-associated vectors in the following formats

  • Small AAV preps for in-vitro use (volume-100uL, at the concentration 1×1011 vg/mL)

Great for the transduction into transformed cell lines from human and rodent-origins. Vectors of this grade will be suitable only for in-vitro studies (not suitable for the transduction into neuron cells). Vectors will be supplied in the aliquots of 20 uL. Vectors will be titered by real-time PCR or by counting reporter-expressed cells.

Production time: 7 days.

  • Large AAV preps for in-vitro use (volume-1 mL, at the concentration 1×1011 vg/mL)

Great for the transduction requiring many samples. Will efficiently transduce transformed cell lines from human and rodent-origins. Vectors of this grade will be suitable only for in-vitro studies (not suitable for transduction into neuron cells). Vectors will be supplied in the aliquots of 100 uL. Vectors will be titered by real-time PCR or by counting reporter-expressed cells.

Production time: 7 days.

 

  • Small AAV Preps for in-vivo use (volume-100uL, at the concentration  2.5 x1012 vg/mL)

 

This grade is suitable for transduction into delicate cells, such as neurons and for in vivo studies. This vector grade will greatly fit small in-vivo pilot experiments. Vectors will be supplied in the aliquots of 20 uL. Vectors will be tittered by real-time PCR, or by counting reporter-expressing cells.

Production time: 2 weeks.

 

  • Mid (Standard) AAV Preps for in vivo use (volume-500 uL, at the concentration  2.5 x1012 vg/mL).

 

This grade is suitable for the transduction into delicate cells, such as neurons and for in vivo studies. This vector grade will greatly fit large in-vivo experiments. Vectors will be supplied in the aliquots of 100 uL. Vectors will be titered by real-time PCR, or by counting reporter-expressing cells.

Production time: 2 weeks.

 

GRADES TABLE

 

 

Prep Vector Titer/Quantity     Time of Production
Small for In Vitro Use ≥1×1011 vg/mL, total 100uL      7 days
Large for In Vitro Use ≥1×1011 vg/mL, total 1mL     7 days
Small for In Vivo Use ≥2.5×1012 vg/mL, total 100uL    2weeks
Standard for In Vivo Use ≥2.5×1012 vg/mL, total 500uL    2weeks

 

 

  • DVVC cannot guarantee production yields if size of the vector cassette exceeds 4.8kbs