The CIPHERS team is proud to announce that the findings from our pilot study have been published in the journal Epigenetics!
“Experiments in rats and a study with 24 men found that THC appears to target genes in two major cellular pathways and alters DNA methylation, a process essential to normal development.
The researchers do not yet know whether DNA changes triggered by THC are passed to users’ children and what effects that could have. Their findings were published online Dec. 19 in the journal Epigenetics.
‘What we have found is that the effects of cannabis use on males and their reproductive health are not completely null, in that there’s something about cannabis use that affects the genetic profile in sperm,’ said Scott Kollins, Ph.D., professor in psychiatry and behavioral sciences at Duke and senior author of the study.
‘We don’t yet know what that means, but the fact that more and more young males of child-bearing age have legal access to cannabis is something we should be thinking about,’ Kollins said.
The higher the concentration of THC in the men’s urine, the more pronounced the genetic changes to their sperm were, the authors found.
THC appeared to impact hundreds of different genes in rats and humans, but many of the genes did have something in common — they were associated with two of the same major cellular pathways, said lead author Susan K. Murphy, Ph.D., associate professor and chief of the Division of Reproductive Sciences in obstetrics and gynecology at Duke.
One of the pathways is involved in helping bodily organs reach their full size; the other pathway involves a large number of genes that regulate growth during development. Both pathways can become dysregulated in some cancers.
‘In terms of what it means for the developing child, we just don’t know,’ Murphy said. It’s unknown whether sperm affected by THC could be healthy enough to even fertilize an egg and continue its development into an embryo, she said.
The study was a starting point on the epigenetic effects of THC on sperm and is limited by the relatively small number of men involved in the trial, Murphy said. The findings in men also could be confounded by other factors affecting their health, such as their nutrition, sleep, alcohol use and other lifestyle habits.
The Duke team plans to continue its research with larger groups. They intend to study whether changes in sperm are reversed when men stop using marijuana. They also hope to test the umbilical cord blood of babies born to fathers with THC-altered sperm to determine what, if any epigenetic changes, are carried forward to the child.
‘We know that there are effects of cannabis use on the regulatory mechanisms in sperm DNA, but we don’t know whether they can be transmitted to the next generation,’ Murphy said.
‘In the absence of a larger, definitive study, the best advice would be to assume these changes are going to be there,’ Murphy said. ‘We don’t know whether they are going to be permanent. I would say as a precaution, stop using cannabis for at least six months before trying to conceive.’
In addition to Kollins and Murphy, study authors include Nilda Itchon-Ramos, Zachary Visco, Zhiqing Huang, Carole Grenier, Rose Schrott, Kelly Acharya, Marie-Helene Boudreau, Thomas M. Price, Douglas J. Raburn, David L. Corcoran, Joseph E. Lucas, John T. Mitchell, F. Joseph McClernon, Marty Cauley, Brandon J. Hall, and Edward D. Levin.”