Pooled CRISPR sgRNA screening
Libraries:
- Custom libraries available in a variety of vectors
- Human Brunello in lentiCRISPR.v2: 76,441 sgRNAs targeting 19,114 genes
- Human Kinome in lentiCRISPR.v2: 6,104 sgRNAs targeting 763 kinase genes
- Mouse Brie in lentiCRISPR.v2: 78,637 sgRNAs targeting 19,674 genes
- Human GeCKO v2: 123,411 lentiviral sgRNA vectors targeting 19,050 genes (Sanjana et al , Nat. Methods 2014)
- Mouse GeCKO v2: 130,209 lentiviral sgRNA vectors targeting 20,611 genes (Sanjana et al , Nat. Methods 2014)
- 73,151 lentiviral sgRNA vectors targeting 7,117 human genes, available as subpools based on functional categories (Wang et al, Science 2014)
- Human CRISPRa (Calabrese): 56,762 (Set A), 56,476 (Set B) lentiviral sgRNA vectors targeting >18,000 transcript isoforms for gain-of-function screening (Sanson et al, Nat. Commun. 2018))
For more details, please send inquiries to rnai@duke.edu.