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CRISPR genome editing services

The facility offers services for custom cell line engineering using CRISPR-based strategies, as well as sgRNA/Cas9 vector construction. Pools or clonal lines of genetic knockouts or knockins can be generated for most commonly used cell lines, using a variety of strategies including RNP nucleofection, lentiviral transduction or transient transfection.

The facility also supports high-throughput functional screening using pooled lentiviral sgRNA libraries against the human and mouse genomes or kinomes. We can also work with the screener to build and screen custom libraries that can be assembled by the screener. Please visit for more details.