The Facility now offers custom CRISPR sgRNA vector construction for genome editing in human and mouse cells and knockout cell line creation services. Vector construction services consist of sgRNA design, vector cloning and experimental validation of efficiency in test cell lines. We have transfection-based and lentiviral-based vectors available. We can also create clonally-derived, sequence validated CRISPR-mediated knockout cells from many commonly used cell lines.
The Facility also supports high-throughput functional screening using pooled lentiviral sgRNA libraries against the human and mouse genomes. Please visit for more details.