This whole-genome siRNA library targets approximately 22,000 human genes. Each gene is targeted by 4 independent siRNAs. The Qiagen Human whole-genome library exists in the Duke Functional Genomics Shared Resource: 1) A 1×4 version of the library comprised of four individual siRNA duplexes targeting 22,000 genes in four 74-plate sets (A, B, C, and D), 2.) A 2×2 pooled version of the library comprised of two 74-plate sets with two siRNAs/well targeting the same gene (A+B and C+D). This is the format most commonly used for screening.
The Qiagen siRNA library can also be screened as subsets, including druggable genes, kinases, phosphatases, and GPCRs. Custom gene sets can also be assembled according to screener requests.