CCSB-Broad lentiviral kinome ORF expression library
The facility has an ORF expression library of 600 kinases, in a lentiviral vector. Each kinase is V5-tagged at its C-terminus for easy tracking of expression. The lentiviral vector, pLX304, drives ORF expression from the CMV promoter and confers blasticidin resistance to transduced cells. This library can be screened in either arrayed or pooled lentiviral format.
Sigma TRC3 LentiORF library
The facility also has a pooled library of 17,000 lentiviral human ORFs in the pLX317 vector, with dedicated barcodes, that can be used for ORFeome-scale pooled screening.
Johannessen, C et al, COT drives resistance to RAF inhibition through MAP kinase reactivation, Nature2010.
Yang X et al, A public genome-scale lentiviral expression library of human ORFs, Nat Methods 2011.
Serra V et al, RSK3/4 mediate resistance to PI3K pathway inbhitors in breast cancer, JCI 2013.