Services
Comprehensive Clinical Flow Cytometry and Immunoassay Services:
Our staff-assisted clinical flow cytometry and Immunoassay services include the following:
Human Immunophenotyping for Clinical Trials: This is a fully-assisted service for advanced screening of immunologic signatures to predict clinical outcomes. This service includes staff-assisted sample processing (thawing, counting, stimulation [where applicable], staining, and analysis, with rigorous QA/QC oversight.
Clinical Cytometry Consultations: We provide expert guidance on study design, sample collection, data analysis, and cost estimates for clinical projects of all sizes and scope.
Biorepository and Sample Processing: We follow standardized protocols for short- and long-term storage, and optimal recovery of clinical samples, including tumor biopsies and blood specimens.
Luminex assays: Staff-assisted immunoassay services are available, with a wide range of analytes to choose from.
Please contact Javid Mohammed for an initial consultation to discuss your clinical cytometry needs.
Assisted Acquisition & Analysis for User-Processed Samples:
We provide assisted analysis services to run samples that are processed and stained by the users. To ensure instrument and staff availability, we request users to schedule assisted appointments well in advance (at least a week or two in advance if possible). Please contact us to consult about, and schedule assisted analysis services. Assisted Cell Sorting:
First time users who are requesting assisted cell sorting service (or anytime there is a change in the Biosafety-level of the samples) are required to complete our online Biosafety and Cell Sorting Questionnaire here Also, we ask first time assisted cell sorting users to consult with us and discuss sample preparation, panel information, collection options, length of sort-time needed, downstream application, etc. prior to scheduling the first sort. Please contact Javid Mohammed for general cell sorting queries, Lynn Martinek and Brittney Ferrari for Astrios sorters, Bin Li for FACSDiscover S8 sorter, and Yingqiu Guo for Sony MA900 and SH800 cell sorter related queries. Experiment Design/Consultation:
We provide free consultation service for all Duke members for experiment and panel design, and for troubleshooting. Please contact Javid Mohammed for experiment design and panel design consultations, and for help with data analysis. Analysis Software:
Windows workstation is located in Jones#307. We currently have FlowJo and SpectroFlo installed on it. A reservation through CoreResearch is required to use the workstation. There is no charge for use, but a fund code is needed to schedule time. Please contact us if you need assistance with data analysis. Training for Independent use of analyzers and SONY cell sorters:
All users are required to go through formal instrument training by our staff members before independent use. Users trained by other users will not be granted access to the instruments for independent use. Please email dciflow@duke.edu to request analyzer and cell sorter (Sony SH800 and Sony MA900) trainings.
Our staff-assisted clinical flow cytometry and Immunoassay services include the following:
We provide assisted analysis services to run samples that are processed and stained by the users. To ensure instrument and staff availability, we request users to schedule assisted appointments well in advance (at least a week or two in advance if possible). Please contact us to consult about, and schedule assisted analysis services. Assisted Cell Sorting:
First time users who are requesting assisted cell sorting service (or anytime there is a change in the Biosafety-level of the samples) are required to complete our online Biosafety and Cell Sorting Questionnaire here Also, we ask first time assisted cell sorting users to consult with us and discuss sample preparation, panel information, collection options, length of sort-time needed, downstream application, etc. prior to scheduling the first sort. Please contact Javid Mohammed for general cell sorting queries, Lynn Martinek and Brittney Ferrari for Astrios sorters, Bin Li for FACSDiscover S8 sorter, and Yingqiu Guo for Sony MA900 and SH800 cell sorter related queries. Experiment Design/Consultation:
We provide free consultation service for all Duke members for experiment and panel design, and for troubleshooting. Please contact Javid Mohammed for experiment design and panel design consultations, and for help with data analysis. Analysis Software:
Windows workstation is located in Jones#307. We currently have FlowJo and SpectroFlo installed on it. A reservation through CoreResearch is required to use the workstation. There is no charge for use, but a fund code is needed to schedule time. Please contact us if you need assistance with data analysis. Training for Independent use of analyzers and SONY cell sorters:
All users are required to go through formal instrument training by our staff members before independent use. Users trained by other users will not be granted access to the instruments for independent use. Please email dciflow@duke.edu to request analyzer and cell sorter (Sony SH800 and Sony MA900) trainings.
