Training/Resources

Instrument Training for Independent Use: All first-time users must go through formal hands-on training provided by our staff. Instrument training by other fellow lab members is not acceptable, and such users will not be granted access to the instruments. Please contact Javid Mohammed for scheduling analyzer trainings, and Yingqiu Guo for Sony SH800 and MA900 cell sorter training.

Initial Cell Sorting Consultation: First time users who are requesting assisted cell sorting must schedule a half-an-hour initial cell sorting consultation with us to discuss sample preparation, panel information, collection options, length of sort-time needed, downstream application, etc., prior to scheduling assisted cell sorting services. Please contact Javid Mohammed for initial cell sorting consultation. We do not charge any fee for this consultation.

Training Material and Guidelines: We ask users to refer to the following respective training material and guidelines prior to attending hands-on training and/or staff-assisted cell sorting services. These materials can only be accessed through Duke Login. If you are prompted to login to access a file here, then please do so using your Duke NetID. Please contact us if you are unable to access any files here, and to request a copy by email.

DCI-FCSR Training Handouts and Sort Guidelines:

Users requesting instrument hands-on training (for self-use) are required to refer to the following respective training guides prior to the hands-on training:
- Cytek Aurora Training Guide
- Fortessa X20 Training Guide
- FACSCanto A/B Training Guide
- FACSCanto HTS Training Guide
- Sony SH800 Cell Sorter Training Guide
- Sony MA900 Cell Sorter Training Guide
- DCI-FCSR Cell Sorting Guidelines: This document provides general guidelines and best practices for sample preparation, and all things related to the cell sorting.

Flow Cytometry Tutorials & Protocols:

- Introduction to Flow Cytometry (Video Tutorial)
- Introduction to Fluorescence (Video Tutorial)
- Anatomy of Fluorescence Spectra (Video Tutorial)
- Overview of Filters and Light Sources (Video Tutorial)
- Introduction to Flow Cytometry Data Analysis (Video Tutorial)
- Doublet Discrimination Tutorial (Video tutorial that covers various strategies to exclude doublets)
- Flow Cytometry Compensation (Video tutorial that covers why/how the compensation is done)
- Cyto University (CytoU Webinars are free to ISAC members)
- Tips and Tricks for Designing Flow Cytometry Panels
- Flow Cytometry Protocols from Biorad

-FlowJo Tutorials:

- Introduction to FlowJo-2024 (Training Seminar Recording from 2024)
- FlowJo Compensation-2024 (Training Seminar Recording from 2024)
- FlowJo High Parameter Data Analysis-2024 (Training Seminar Recording from 2024)
- FlowJo Basics-2023 (Training Seminar Recording from 2023)
- FlowJo High Parameter Data Analysis-2023 (Training Seminar Recording from 2023)

Flow Cytometry Tools:

- FluoroFinder Panel Design Software (DCI-FCSR instruments can be found in their database)
- Cytek Full Spectrum Viewer (This tool comes in handy when designing your panels to run on our 5-laser, 64-color Cytek Aurora Spectral Analyzer)

Useful Flow Cytometry Links:

- The Flow Cytometry Network: A network to connect with the cytometry community
- International Society for Advancement of Cytometry
- Optimized Multicolor Immunofluorescence Panels (OMIPs)
- Minimum Information about a Flow Cytometry Experiment (MIFlowCyt): Guidelines for publishing flow cytometry data
- Rare-Event Detection Using Flow Cytometry (Tools and Strategies)