The Kinesin-8 (formerly Kip3) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-8 proteins from humans and several model organisms. Kinesin-8 proteins from other organisms are also shown in the table and sequence alignment below.
| Species/protein | Molecular mass (kDa) | Motor polarity & velocity | Subcellular localization | Comments |
|---|---|---|---|---|
| S. cerevisiae KIP3 | 91 | ND | ND | |
| S. pombe BC2F12.13 | 99 | ND | ND | |
| S. pombe BC649.01c | 88 | ND | ND | |
| D. melanogaster Klp67A | ND | ND | ||
| P. falciparum MAL3P7 | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360
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ScKIP3 RNPLNKVSDQVLNSMRARATKATASSINNSNATNKFSSQRR-------------------RHGGEIKFVFDKLFDETSSQARVYKETTSPLLDSVLDGFNSTVFAYGATGCGKTYTVSGTPSQPGIIFLAMEELFNKITDLKDEKDFEISLSYLEIYNERIRDLLKPET----PSKRLVIREDTQNHIKVANLSYHHPNTVEDVMDLVVQGNINRTTSPTEANEVSSRSHAVLQIHIMQTN-------KLVDLTSQHTFATLSIIDLAGSERAAATRNRGIRLHEGANINRSLLALGNCINALCLNDGSRSCHIPYRDSKLTRLLKFSLGGNCKTVMIVCISPSSSHYDETLNTLKYANRAKEIKT--KIIR ScKIP3
SpoBC2f12 VLVFDPPDETTATLSATNRRLSTSQQSLARLSRKSNNSAGF---------------------GRDLRYAFDRVFDETATQQQVYERTARPLLDNILDGFNATIFAYGATGCGKTHTISGTMQDPGLIYLTLKELFERMDHLRDEKIFDLRLSYLEIYNETIRDLLVSPT--PNQAKPLNLREDADRRITVPGLTSLSPESLEEIIDIIMKGNANRTMSPTEANAASSRSHAVLQVTLIQKP-------RTAGINEDHTLATLSIIDLAGSERATATKLRGSRLFEGANINKSLLALGNCINALCDPHRR--AHVPYRDSKLTRLLKFSLGGNCRTVMIVCVSPSSVHYEETHNTLKYANRAKNIKTE-VLRN SpoBC2f12
SpoBC649 IRKIVRVLDDNVLIFDPPEENPLAKVQKSLLPAGKRF--------------------------RDVRYAFDRLFGEEASQEDVYKGTTEPLLDSVLQGYNATVFAYGATGCGKTHTISGRPDDPGIIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPKSLNICEDAEQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVSSRSHAVLQIYITQTPKSGEKQEESESQNSHKVRSVFSFIDLAGSERASATKNRGKRLVEGANINRSLLALGNCINSLCEPRRR--QHVPYRDSKLTRLLKFSLGGNCRTCMIVCISPSSEHYDETHNTLKYGNRAKNIKTK-VSRN SpoBC649
DmKlp67A QHTNIKVAVRVRPYNVRELEQKQRSIIKVMDRSALLFDPDEEDDEFFFQGAKQPYRDITKRMNKKLTMEFDRVFDIDNSNQDLFEECTAPLVDAVLNGYNCSVFVYGATGAGKTFTMLGSEAHPGLTYLTMQDLFDKIQAQSDVRKFDVGVSYLEVYNEHVMNLLTKSG-------PLKLREDNNGVV-VSGLCLTPIYSAEELLRMLMLGNSHRTQHPTDANAESSRSHAIFQVHIRITE----------RKTDTKRTVKLSMIDLAGSERAASTKGIGVRFKEGASINKSLLALGNCINKLADGL----KHIPYRDSNLTRILKDSLGGNCRTLMVANVSMSSLTYEDTYNTLKYASRAKKIRTT-LKQN DmKlp67A
PfMAL3P7 AYSNVKVAVRIKPIGESEENIVSIFNKNYVLIEKENEKEC----------------YLLSQKKKQSTYVFDSVFDVNATQEEVFFQTAKPLIPHVFKGINCTVFAYGATGSGKTYTMLDDKNQNGIVQLSLLELFTIINEKKCRN-IKVLMSFLEVYNETIRDLLGKEK------NKTLEVQEDVAEVKVSNLCEIEVNNYEQAMLLINEGVKNRKMSPTRANKVSSRSHAILQIYVYNE-------ILDDNMNTISYKAKLCFVDLAGSERASATSNKGERFKEGSYINQSLLALANCINSLASNRNISKVRVKYRDSKLTHLLKNSLEGNCLVVMIANINPSRTSFQESNNTLKYAFRARNIKLCATVQT PfMAL3P7
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 15 January 1999 20:30 GMT
Modified 25 January 2005 7:26 GMT
