The Kinesin-4 Family

The Kinesin-4 (formerly Chromokinesin/KIF4) family tree was obtained using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-4 proteins from humans and several model organisms. Kinesin-4 proteins from other organisms are also shown in the table and sequence alignment below.

Species/protein Molecular mass (kDa) Motor polarity & velocity Subcellular localization Comments
D. melanogaster Klp3A ND
H. sapines KIF4 140 ND
D. melanogaster Klp31E ND
H. sapiens NYREN62 70
C. elegans T01G1 ND
A. thaliana MCA23.16 117
A. thaliana F11C1.80 121
A. thaliana MSL3.5 145
C. elegans Y43F4B ND
D. melanogaster Klp31D ND
G. gallus Chrkin 139 ND Chromosome arms (chromokinesin) Binds DNA
M. musculus KIF4 140 Plus, 2-12 µm/min Membranous vesicles, mitotic spindle Motor for vesicle transport?
X. laevis Klp1 139 ND Chromosome arms May position mitotic chromosomes, stabilize bipolar mitotic spindle
          1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360      
          '        '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '       
DmKlp3A   DPSCVAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKDLFETCVQAKVKKLLNGYNVTILAYGQTGSGKTYTMGTAFN--GVLDDHV-GVIPRAVHDIFTAIAEMQ-SEF-----------RFAVTCSFVELYQEQFYDLFSSKT-RDKATVDIREVKNRIIMPGLTELVVTSAQQVTDHLIRGSAGRAVAATAMNETSSRSHAIFTLTLVATK-------------LDGKQSVTTSRFNLVDLAGSERCSKTLASGDRFKEGVNINKGLLALGNVINALGSG-QAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVAETLSTLRYADRALQIKNKPVVN  Dmklp3A 
HsKIF4    KGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGT-DKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTV-GVIPRVIQLLFKEIDKKSDFEF-------------TLKVSYLEIYNEEILDLLCPSR-EKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAILTISLEQGK-------------KSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDD-KKGGFAPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVN  HsKIF4
DmKlp31E  KDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGS-DKAFTFDYVFDTNSNQCDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFN-KNSNFKIHEDASGQITISGASIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQR-----LLTPSDNVPDNDLETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKIN  DmKlp31E
CeT01G1   NDRDLISVKSIRPQGNREKLEGSRVCTSVLPNDPQVTIGG-DRSFTYDHVFDMPTLQYVVYESCVEKLVDGLFDGYNATVLAYGQTGSGKTHTMGTAFDAAVTQKEED-LGVIPRAIQHTFRKIAECKAQA---IEQGLLEPAFEVSVQFVELYNDDVLDLLSDDRSMSSSIRIHEDSRGEIVLHGVEQRSVFDMHGTMDILKNGALNRTVAATNMNEQSSRSHAIFTLHLKQQRVAAPLDESGEQKTGELEMEMLCAKFHFVDLAGSERMKRTGATGDRAKEGISINVGLLALGNVIAALGGANGKVSHVPYRDSKLTRLLQDSLGGNSRTLMIACCSPMDSDFVETLNTMKYANRAKEIKNKVVAN  CeT01G1
CeY43F4B  SDASLRVVVRARPMNGRETKEGASRCVQFYENTKQIVINE-SATFTFDAVFADTSDQESVYETTALPLLDRIFAGFNATVLAYGQTGSGKTYTMGTEDN--VGTDEMRRGIIPRLVSALFQRIMNTE-APE-----------SFAVTVSMFEVYGDNVYDLLRPDK----VKLNVHGDEKNCTVVNLTAVPVIDLKGALKQLAVGCHYRTKAETAMNAMSSRSHAVFTVFVEKTA-------------TAECDSAFSAKLQLVDLAGSERLKKTEAEGNRMKEGININGGLLILSQVIAALAT---KQKHIPYRNSVITRVLQDSLGGNSFTVFLACISPADSNSQETLNTLRYADRAKQIKNKPIVN  CeY43F4B 
DmKlp31D  KDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGS-DKAFTFDYVFDTNSNQCDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFN-KNSNFKIHEDASGQITISGASIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQR-----LLTPSDNVPDNDLETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKIN  DmKlp31D
Ggchrkin  EKGIPVRVVRCRPLVPKETSEGCQMCLSFVPGEPQVIVGS-DKAFTYDYVFDPSVEQEEVFNTAVAPLIRGIFKGYNATVLAYGQTGSGKTYSMGGTYTASQEHDPSM-GVIPRVIKLLFKEKEQRQDWEF-------------VLKVSYLEIYNEDILDLLCSSRERSSQISIREDPKEGIKIVGLTERNVASARDTVSCLEQGNNCRTVASTAMNSQSSRSHAIFTICIDQKK-------------KNDKNSSFHSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGEENKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVN  Ggchrkin
MmKIF4    KGIPVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGN-DKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAI-GVIPRVIQLLFKEINKKSDFEF-------------TLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGLTEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRK-------------KNDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGDD-KKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIIN  MmKIF4
Xlklp1    EGIPVRVALRCRPLVPKENNEGCKMCLTFVPGEQQVIVGT-EKSFTYDYVFDPSAEQEEVYNSAVAPLIKGLFKGYNATVLAYGQTGSGKTYSMGGAYTHNQENEPTV-GVIPRTVIALFREIHQRPEWEF-------------NLKVSYLEIYNEEILDLLYAARDKTNTISIREDPKEGIKICGLTERDVKTALDTLSCLEQGNSSRTVASTAMNSQSSRSHAIFTISIEQRK-------------EGDKNNSFRSKLHLVDLAGSERQKKTKAEGDRLKEGISINRGLLCLGNVISALGDESKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNMEETLNTLRYADRARKIKNKPIVN  Xlklp1

If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.

Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:23 GMT