The Kinesin-3 (formerly Unc104) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-3 proteins from humans and several model organisms. Kinesin-3 proteins from other organisms are also shown in the table and sequence alignment below.
| Species/protein | Molecular mass (kDa) | Motor polarity & velocity | Subcellular localization | Comments |
|---|---|---|---|---|
| H. sapiens Kif16B | 45 | |||
| D. melanogaster Klp98A | ND | ND | ||
| D. melanogaster Klp73 | ND | ND | ||
| H. sapiens GAKIN | 203 | |||
| H. sapiens RBKIN1 | 202 | |||
| C. elegans F56E3 | ND | ND | ||
| C. elegans Unc-104 | 180 | ND | ND | Functions in synaptic vesicle transport |
| H. sapiens KIF1B | 204 | |||
| H.sapiens ATSV | 191 | ND | ND | |
| H.sapiens KIF1C | 123 | ND | ND | |
| D. melanogaster Klp53D | ND | ND | ||
| C. elegans KLP6 (LR144) | ND | ND | ||
| D. melanogaster Klp38B | ND | ND | ||
| H.sapiens CMKRP | ND | ND | ||
| M. musculus KIF1A | 192 | Plus, 72 µm/min | Enriched in axons | Associated with synaptic vesicles, monomeric? |
| M. musculus KIF1B | 130 | Plus, 40 µm/min | Co-localizes with mitochondria | Transports mitochondria, monomeric? |
| R. norvegicus KIF1B | 77 | ND | ND | |
| R. norvegicus KIF1D | 122 | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430
' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' '
DmKlp98A MDAQLIMEMENKKTRLLKPRLQSIRDAGRDNHHDFTFDYS---------YWSFDAEDPHFA-------------TQEQVYSDLGNDVVDCAYEGYNACVFAYGQTGSGKTFTMMGT-------------PNNPGLIPRICEELFARMRVGQESGTG-YRTHASYLEIYNERVKDLLAAQS-----------------TGHGLRVREHRSLGPYVENLSQHAVSDFDEIQECIARGNAQRTTASTNMNDTSSRSHAIFTITFVQAV--------------FMNDMPSETVSKIHLVDLAGSERANATGATGQRLKEGAHINKSLVTLGSVISALAEQTGGGHN------------------------------SSSSYIPYRDSILTWLLKDSLGGNSKTIMIAALSPADCNYSETLSTLRYANRAKNIINKPTVN DmKlp98A
DmKlp73 ASDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPP-PLEKIERKQPKTFAFDHCFYSLN---PEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGT-------------QESKGIIPRLCDQLFSAIANKSTPELM-YKVEVSYMEIYNEKVHDLLDPKP-----------------NKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQIL------------TDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNG------------------------------KKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVN DmKlp73
CeF56E3 EESAVKVAIRVRPFNKRELDLKTKSVVRIQKEQCVLHHPI------EEKNSKTFTFDHSFCSTD---PHSYDFASQETVSYHLGSGVVENAFSGYNACIFAYGQTGSGKSYTMMGT-------------PDQPGIIPRVCNDIFTRIQETSNSSLS-FKVEVSYMEIYNERVRDLLDPKK-----------------SSKALKVREHKILGPMVDGLSILAVNSFEQISNLLEEGNKSRTVAATNMNAESSRSHAVFSLIVTQTL------------HDLENGFSGEKVAKISLVDLAGSERAGKTGAVGKRLEEGGNINKNLVSIFLRNDLEKKIDFKFSADVYVFINQKLDFRSLTTLGMVISALAERNSKKDKFIPYRDSVLTWLLKDSLGGNSRTVMIATLSPAADNYEETLSTLRYADRAKKIVNHAIIN CeF56E3
CeUNC104 MSSVKVAVRVRPFNQREISNTSKCVLQVNGNTTTINGHS------INKENFSFNFDHSYWSFA--SRNDPHFITQKQVYEELGVEMLEHAFEGYNVCIFAYGQTGSGKSYTMMGK----------ANDPDEMGIIPRLCNDLFARIDNNNDKDVQ-YSVEVSYMEIYCERVKDLLNPNS------------------GGNLRVREHPLLGPYVDDLTKMAVCSYHDICNLMDEGNKARTVAATNMNSTSSRSHAVFTIVLTQKR------------HCADSNLDTEKHSKISLVDLAGSERANSTGAEGQRLKEGANINKSLTTLGLVISKLAEEST------------------------------KKKKSNKGVIPYRDSVLTWLLRENLGGNSKTAMLAALSPADINFDETLSTLRYADRAKQIVCQAVVN CeUNC104
HsATSV AGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPK-----QPKETPKSFSFDYSYWSHT--SPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGK-----------QEKDQQGIIPQLCEDLFSRINDTTNDNMS-YSVEVSYMEIYCERVRDLLNPKN------------------KGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKR------------HDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPN--------------------------KNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVIN HsATSV
HsKIF1C AGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSIINPK-----QSKDAPKSFTFDYSYWSHT--STEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGR-----------QEPGQQGIVPQLCEDLFSRVSENQSAQLS-YSVEVSYMEIYCERVRDLLNPKS------------------RGSLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRC------------HDQLTGLDSEKVSKISLVDLAGSERADSSGARGMRLKEGANINKSLTTLGKVISALADMQS--------------------------------KKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLSTLRYADRTKQIRCNAIIN HsKIF1C
DmKlp53D KMSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPK-----VPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGR-----------QEEQQEGIIPMICKDLFTRIQDTETDDLK-YSVEVSYMEIYCERVRDLLNPKN------------------KGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRR------------HDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVAS-----------------------------KKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVN DmKlp53D
Dm38B EESNMIVAVRVRPLNALECTRGQVTNVVQVHGNSNELTVQ---AGSSADASAGVTHFFSYDQVYSCDPERKNFACQAKVFEGTARPLIDTAFEGYNACLFAYGQTGSGKSYSMMGIEALDDAALDGGPPHDEAGIIPRFCHELFRRIEAVKSQQQLQVEVEVSYFEIYNEKIHDLLSVQHAAAATGESTPIQQQQQQQRPALKVREHPIFGPYVVDLSAHSVDSYSALRNWLAVGNSQRATASTAMNDKSSRSHSIFNIVLNLTDLSSDDGLSSDTDSSTASSLRQTRRSKISLVDLAGSERISVSGSNGERIREGVSINKSLLTLGKVIAALADSRKASANGPLGSGTP------------------------STFVPYRESVLTWLLRENLGGNSKTVMLATISPASIHADETLATLRYACKARSIVNRVKVN Dm38B
HsCMKRP ENSQVTVAVRVRPFTKREKIEKASQVVFMSGKEITVEHPDTKQVYNFIYDVSFWSFDECHPHYA----------SQTTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSYTMMGF-------------SEEPGIIPRFCEDLFSQVARKQTQEVS-YHIEMSFFEVYNEKIHDLLVCKD-------------ENGQRKQPLRVREHPVYGPYVEALSMNIVSSYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQTK----------TEFVEGEEHDHRITSRINLIDLAGSERCSTAHTNGDRLKEGVSINKSLLTLGKVISALSEQA----------------------------------NQRSVFIPYRESVLTWLLKESLGGNSKTAMIATISPAASNIEETLSTLRYANQARLIVNIAKVN HsCMKRP
CeLR144 MPDEETTVIRDPKTNDEKRFTYDHSYWSHDGFS------------EKKNGYLEPTDPHYA-------------DQRRVFEDLGRGVLANAWAGYNCSLFAYGQTGSGKSYSIVGF-------------KNNKGIVPIVCEELFKQIADNKKKNMQ-FEVFVSMMEIYCEKVRDLLSSTP----------------PPKGGLKVREHPKNGFYVENLTTVPVNSFKEIEAKIEEGTKSRTIAATQMNATSSRAHTIVKITFNQKS-------------SKQAGGTSMKKSEINLVDLAGSERQSAAGTEGDRLKEGIVINQSLTTLGRVIKALHDSQ-------------------------------KAKSGKKTQIPYRDSVLTCLLKNALGGNSKTIMIAAISPADINFEETLSTLRFADRAKSIKTNAVVN CeLR144
MmKIF1A AGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPK-----QPKETPKSFSFDYSYWSHT--SPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGK-----------QEKDQQGIIPQLCEDLFSRINDTTNDNMS-YSVEVSYMEIYCERVRDLLNPKN------------------KGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKPRTVAATNMNETSSRSHAVFNIIFTQKR------------HDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPN--------------------------KNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAIIN MmKIF1A
MmKIF1B SGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPK-----NPKEAPKSFSFDYSYWSHT--SPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGK-----------QEESQAVIIPQLCEELFEKINDNCNEEMS-YSVEVSYMEIYCERVRDLLNPKN------------------KGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKK------------QDPETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVS--------------------------------KKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVIN MmKIF1B
RnKIF1b SGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPK-----NPKEAPKSFSFDYSYWSH---SPEDPCFASQSRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGK-----------QEESQAGIIPTCCEELFEKINDNCNEDMS-YSV-SSYMEIYCERVRDLLNPKN------------------KGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKK------------QDPETNLSTEKVTKISLVDLAGSERADSTGAKGTRLKEGRNINKSLTTLGKVISALAEVS--------------------------------KKKKKTDFIPYRDSVLTWLLREN-GGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVIN RnKIF1b
RnKIF1d AGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSIINPK------QSRMFLKASFDYSYWSH--TSVEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGR-----------QEPGQQGIVPQLCEDLFSRVNVNQSAQLS-YSVEVSYMEIYCERVRDLLNPKS------------------RGSLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRS------------HDQLTGLDSEKVSKISLVNLAGSERADSSGARGMRLKEGANINKSLTTLGKVISALADLQS--------------------------------KKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLSTLRYADRTKQIRCNAVIN RnKIF1d
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:22 GMT
