The Kinesin-2 (formerly KRP85/95 or Kinesin II) family tree was found using the heuristic search method of PAUP v4.0b4a (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-2 proteins from humans and several model organisms. Kinesin-2 proteins from other organisms are also shown in the table and sequence alignment below.
| Species/protein | Molecular mass (kDa) | Motor polarity & velocity | Subcellular localization | Comments |
|---|---|---|---|---|
| D. melanogaster Klp64D | ND | ND | ||
| H. sapiens KIF3 | 45 | ND | ND | |
| C. elegans Y50D7 | ND | ND | ||
| H. sapiens KIF3B | 85 | ND | ND | |
| H. sapiens KIF3C | 89 | ND | ND | |
| C. elegans KLP11 (F20C5) | 129 | ND | ND | |
| D. melanogaster Klp68D | 88 | Plus, 18 µm/min | ND | Present in CNS, may be a motor for anterograde axonal transport |
| H. sapiens KIAA1405 | 115 | |||
| C. elegans Osm3 | 75 | ND | ND | Required for cilia function, transport of vesicles in sensory apparatus |
| D. melanogaster Kif3C | 66 | |||
| C. reinhardtii FLA10 | 87 | ND | Flagella | Role in flagellar assembly/maintenance |
| M. musculus KIF3A | 80 | Plus, 36 µm/min | Microsomes/ synaptic vesicles | Putative heterotetrameric motor for fast axonal transport |
| M. musculus KIF3B | 85 | Plus, 18 µm/min | Neurons, cell bodies, axons and dendrites | Associated with anterograde moving organelles transported by fast axonal flow |
| M. musculus KIF3C | 90 | ND | ND | |
| R. norvegicus KIF3C | 90 | ND | ND | |
| S. purpuratus KRP85 | 79 | Plus, 24 µm/min | ND | Heterotrimer with KRP95 and non-KRP |
| S. purpuratus KRP95 | 84 | Plus, 24 µm/min | ND | Heterotrimer with KRP85 and non-KRP |
| T. thermophila Kin1 | ||||
| T. thermophila Kin2 | ||||
| X. laevis Klp3 | ND | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400
' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' '
DmKlp64D EIENVRVVVRTRPMDKNELSAGALSAISVDKINRAITVMKPN---------------ATANEPPKTYYFDNVFDGGS--------NQMDLYVDTARPIVDKVLEGYNGTILAYGQTGTGKTYTMSGNPDSPQTKGIIPNAFAHIFGHIAKAKENQKFLVRVSYMEIYNEEVRDLLGKDVGKSLEVKERPDIGVFVKDLSGYVVHNADDLENIMRLGNKNRAVGATKMNQESSRSHAIFSITVERSE-----------LGEGDVQHVRMGKLQLVDLAGSERQSKTQ--------------------------ASGQRLKEATKINLSLSVLGNVISALVDGKSTHIPYRNSKLTRLLQDSLGGNSKTVMCATISPADSNYMETISTLRYASRAKNIQNRMHIN DmKlp64D
HsKIF3 SCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTD----------------SSNEPPKTFTFDTVFGPES--------KQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLAVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSE-----------KGIDGNMHVRMGKLHLVDLAGSERQAKTG--------------------------ATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARIN HsKIF3
CeY50D7 GAEKVKVVVRCRPISTTEKLQGHKIAVTCNDEEKAVNIKSLS-----------------QEDPPRTFYFDAVFSPNT--------DQMTVYNVAARPIVENVLKGYNGTIFAYGQTGTGKTFTMAGDLEPVEMRGIIPNSFAHIFDHIAKCQHDTTFLVRVSYLEIYNEEIRDLLSKDHNGNLEIKERPDVGVYVRNLSNPTVENASKMQALMEFGSKNRKVGATAMNLESSRSHAMFTVTIESCR----------------NGLVTQGKLQLVDLAGSERQSKTG--------------------------AQGERLKEAAKINLSLSTLGNVISSLVDGKSTHIPYRNSKLTRLLQDSLGGNSKTVMIANVGPATYNYDETLSTLRYANRAKNIQNVAKIN CeY50D7
HsKIF3B SSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPK---------------GTAHEMPKTFTFDAVYDWNA--------KQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSE-----------VGLDGENHIRVGKLNLVDLAGSERQAKTG--------------------------AQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVN HsKIF3B
HsKIF3C ASEALKVVARCRPLSRKEEAAGHEQILTMDVKLGQVTLRNPR---------------AAPGELPKTFTFDAVYDASS--------KQADLYDETVRPLIDSVLQGFNGTVFAYGQTGTGKTYTMQGTWVEPELRGVIPNAFEHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKEPGKRLELKENPETGVYIKDLSSFVTKNVKEIEHVMNLGNQTRAVGSTHMNEVSSRSHAIFIITVECSE-----------RGSDGQDHIRVGKLNLVDLAGSERQNKAGPNTAGGAATP--SSGGGGGGGGSGGGAGGERPKEASKINLSLSALGNVIAALAGNRSTHIPYRDSKLTRLLQDSLGGNAKTIMVATLGPASHSYDESLSTLRFANRAKNIKNKPRVN HsKIF3C
CeKLP11 KQETVKVIVRCRPLSSQEIANNYSKIVHMRPQRGQIELKNPK----------------EQDEPSKDFTFDAIYDENS--------TQSDLYEETFRDLVDSVLNGYNATIFAYGQTGTGKTHTMEGKSSDPEQRGVIYKCIDHIFEHMAASHNQE-YLVRASYLEIYQEELRDLLEAESNKKLEIKERPDGGVYVKDLTSKLTRTVGEIHEVMIRGNGHRSVGRTNMNEHSSRSHAIFIITVECSR-----------IGEDGESHITVGRLNLVDLAGSERQSKTG--------------------------ATGERFKEATKINLSLSALGNVISALVDAKSAHIPYRDSKLTRLLQDSLGGNSKT--------------ETLGTLRYANRAKNIKNQPKIN CeKLP11
Dmklp68D PNECVQVVVRCRPMSNRERSERSPEVVNVYPNRGVVELQNVV---------------DGNKEQRKVFTYDAAYDASA--------TQTTLYHEVVFPLVSSVLEGFNGCIFAYGQTGTGKTFTMEGVRGNDELMGIIPRTFEQIWLHINRTENFQ-FLVDVSYLEIYMEELRDLL-KPNSKHLEVRER-GSGVYVPNLHAINCKSVEDMIKVMQVGNKNRTVGFTNMNEHSSRSHAIFMIKIEMCD--------------TETNTIKVGKLNLIDLAGSERQSKTG--------------------------ASAERLKEASKINLALSSLGNVISALAESS-PHVPYRDSKLTRLLQDSLGGNSKTIMIANIGPSNYNYNETLTTLRYGSRAKSIQNQPIKN Dmklp68D
CeOSM3 LELWVRNRGNCRKLYKVFWGTRGLLLGTRDGKALEIDEFLLGTRENVSKFPAIVGMTPNVGQVNLNAPDGAAKDFTFDGAYFMIRPGEQIYNDIVFPLVENVIEGYNGTVFAYGQTGSGKTFSMQGIETIPAQRGVIPRAFDHIFTATATTENVK-FLVHCSYLEIYNEEVRDLLGADNKQKLEIKEQPDRGVYVAGLSMHVCHDVPACKELMTRGFNNRHVGATLMNKDSSRSHSIFTVYVEGIT---------------ETGSIRMGKLNLVDLAGSERQSKTG--------------------------ATGDRLKEATKINLSLSALGNVISALVDGKSKHIPYRDSKLTRLLQDSLGGNTKTIMIACVSPSSDNYDETLSTLRYANRAKNIKNKPTIN CeOSM3
CrFLA10 GSESVKVVVRCRPLNGKEKADGRSRIVDMDVDAGQVKVRNPK---------------ADASEPPKAFTFDQVYDWNC--------QQRDVFDITARPLIDSCIEGYNGTIFAYGQTGTGKSHTMEGKDEPPELRGLIPNTFRYVFEIIARDSGTKEFLVRSSYLEIYNEEVRDLLGKDHSKKMELKESPDRGVYVKDLSQFVCKNYEEMNKVLLAGKDNRQVGATLMNQDSSRSHSIFTITIECIEKLESAAAQKPGAKKDDSNHVRVGKLNLVDLAGSERQDKTG--------------------------ATGDRLKEGIKINLSLTALGNVISALVDGKSGHIPYRDSKLTRLLQDSLGGNTKTVMVANIGPADWNYDETMSTLRYANRAKNIQNKPKIN CrFLA10
MmKIF3A SCDNVKVVVRCRPLNEREKSMCYRQAVSVDEMRGTITVHKTD----------------SSNEPPKTFTFDTVFGPES--------KQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPGLRGVIPNSFAHIFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSE-----------KGVDGNMHVRMGKLHLVDLAGSERQAKTG--------------------------ATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARIN MmKIF3A
MmKIF3B SSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPK---------------GTSHEMPKTFTFDAVYDWNA--------KQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGVRGDPEKRGVIPNSFDHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSE-----------VGLDGENHIRVGKLNLVDLAGSERQAKTG--------------------------AQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVN MmKIF3B
MmKIF3C ASEALKVVARCRPLSRKEEAAGHEQILTMDVKLGQVTLRNPR---------------AAPGELPKTFTFDAVYDASS--------KQADLYDETVRPLIDSVLQGFNGTVFAYGQTGTGKTYTMQGTWVEPELRGVIPNAFEHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKEPGKRLELKENPETGVYIKDLSSFVTKNVKEIEHVMNLGNQARAVGSTHMNEVSSRSHAIFVITVECSE-----------RGSDGQDHIRVGKLNLVDLAGSERQNKAGPNAAGGPATQPTAGGGSGSGSASGSASSGERPKEASKINLSLSALGNVIAALAGNRSTHIPYRDSKLTRLLQDSLGGNAKTIMVATLGPASHSYDESLSTLRFANRAKNIKNKPRVN MmKIF3C
RnKIF3C ASEALKVVARCRPLSRKEEAAGHEQILTMDVKLGQVTLRNPR---------------AAPGELPKTFTFDAVYDASS--------KQADLYDETVRPLIDSVLQGFNGTVFAYGQTGTGKTYTMQGTWVEPELRGVIPNAFEHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKEPGKRLELKENPETGVYIKDLSSFVTKNVKEIEHVMNLGNQARAVGSTHMNEVSSRSHAIFVITVECSE-----------RGSDGQDHIRVGKLNLVDLAGSERQNKAGPNTPGGPATQSTAGGGGGGGGTSGSGSSGERPKEASKINLSLSALGNVIAALAGNRSTHIPYRDSKLTRLLQDSLGGNAKTIMVATLGPASHSYDESLSTLRFANRAKNIKNKPRVN RnKIF3C
SpKRP85 GNDNVRVVVRCRPLNSKETGQGFKSVVKMDEMRGTVQVTNPN---------------APSGEPPKSFTFDTVFAPGA--------KQTDVYNQTARPIVDAIIEGYNGTIFAYGQTGTGKTFTMEGVRSQPELRGIIPNSFAHIFGHIAKEQENVRFLVRVSYLEIYNEEVKDLLGKDQQHRLEVKERPDVGVYVKDLSAFVVNNADDMDRIMTLGNKNRSVGATNMNESSSRSHAIFTITLERSD-----------MGLDKEQHVRVGKLHMVDLAGSERQTKTG--------------------------ATGQRLKEATKINLSLSTLGNVISSLVDGKSTHIPYRNSKLTRLLQDSLGGNAKTVMCANIGPAEYNYDETISTLRYANRAKNIKNKAKIN SpKRP85
SpKRP95 SAETVKVVVRCRPMNSKEISQGHKRIVEMDNKRGLVEVTNPK---------------GPPGEPNKSFTFDTVYDWNS--------KQIDLYDETFRSLVESVLQGFNGTIFAYGQTGTGKTFTMEGVRSNPELRGVIPNSFEHIFTHIARTQNQQ-FLVRASYLEIYQEEIRDLLAKDK-KRLDLKERPDTGVYVKDLSSFVTKSVKEIEHVMTVGNNNRSVGSTNMNEHSSRSHAIFIITIECSE-----------LGVDGENHIRVGKLNLVDLAGSERQAKTG--------------------------ATGDRLKEATKINLSLSALGNVISALVDGKSSHIPYRDSKLTRLLQDSLGGNAKTVMVANMGPASYNFDETITTLRYANRAKNIKNKPKIN SpKRP95
TtKin1 NDECVKVVVRCRPLNSKEKEDGRTQVVFVNQSRGEISVTNPK---------------GDSAEAPKVFTFDSTFEPEV--------EQETVYKNTAYPIVESVLEGYNGTIFAYGQTGTGKTHTMEGKDEPKHLRGIIPRTFDHIFRSIKGTPNVQ-FLVRVSFLELYNEEIRDLLQKNI-KKLELREKPGSGVYVKDLSTFMIQDQEELREKLLHGRENRAVGATQMNQDSSRSHSIFGITIERCD------------IVNGESHIRVGKLNLVDLAGSERQSKTQ--------------------------ATGSRLKEAININQSLTTLGNVISSLIDPKATHVPYRDSKLTRLLQDSLGGNTKTVMVANVGPADFT-MMNYSTLRYAHRAKSIQNHAKIN TtKin1
TtKin2 NSECFKVAVRCRPLNNDEIQNERSSVVSVNPQRGEIQIKQKH----------------DQAEQHRIFAFDQVFEPDI--------QQEVVYKCIAYPIVESVLEGYNGTIFAYGQTGTGKTHTIQGRNEPINERGIIPRAFEHIFHSIKGSPNTQ-FLVHVSFLELYNEEIQDLLSTKN-KKLELREKAETGVFVKDLTSFLVQNEQELNDKFQQGILNRKVGQTKMSSCSSRSHSILSVTIERCD------------VVNGENHIKVGKLNLVDLAGSERQSKTQ--------------------------ATGSRFKEGVYINLSLTTLGNVISSLIDPKASHIPYRDSKLTRILQDSLGGNTKTVMIANIGPADYNQDETISTLRYAHRAKSIQNHAQIN TtKin2
XlKlp3 SSESVRVVVRCRPMNSKELAAGYERVVDVDVKLGQVSVKVHK---------------GATNELSKTFTFVATYDSNS--------NEVELYVETFRPLVDSVLLGLNGTIFATGQTGTGKTYTMEGVRGDPEKRGVIPNSFEHIFTHISRSQNQQ-YLVRASYLEIYQEEIRDLLSKDQSKRLELKERPDIGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFMITIECSQ-----------IGLDGENHIRVGKLNLVDLAGSERQTKTG--------------------------AQGERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNAKTVMVANIGPASYNVEETLTTLRYSNRAKNIKNKPRVN XlKlp3
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:20 GMT
