The Kinesin-13 (formerly MCAK/KIF2) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-13 proteins from humans and several model organisms. Kinesin-13 proteins from other organisms are also shown in the table and sequence alignment below. There are three Kinesin-13 proteins in Drosophila, the mouse and humans. Pairwise comparisons of full-length protein and motor domain sequences identify putative orthologues as [DmKlp10A, MmKif2A, HsKin2 (HsKif2)]; [DmKlp59C, MmKif2C, HsMCAK]; and [DmKlp59D, MmKif2B, HsKlp17] (C.D. Yankel & S.A. Endow, 2006). The Kinesin-13 proteins function in MT disassembly in mitosis and interphase.
| Species/protein | Molecular mass (kDa) | Motor polarity & velocity | Subcellular localization | Comments |
|---|---|---|---|---|
| C. fusiformis DSK1 | 63 | ND | Spindle | |
| P. falciparum L2165w | ||||
| D. melanogaster Klp10A | ND | ND | ||
| D. melanogaster Klp59D | ||||
| D. melanogaster Klp59C | ND | ND | ||
| H. sapiens Klp17q22 | 76 | |||
| H. sapiens Kin2 | 77 | ND | ND | |
| H. sapiens MCAK | 81 | ND | ND | |
| C. elegans K11D9.c | 77 | ND | ND | |
| A. thaliana MGL6.9 | 89 | |||
| A. thaliana MSL1.9 | 77 | |||
| L. major Kin | 74 | ND | ND | |
| C. griseus MCAK | 83 | ND | Kinetochore | Localizes to kinetochore, anchors kinetochore MTs? |
| M. musculus KIF2 | 81 | Plus, 28 µm/min | Membranous vesicles | Abundant in developing axons, transport of specific organlles? |
| M. musculus KIF2C | 75 | ND | ND | |
| R. norvegicus KRP2 | ND | ND | ND | |
| X. laevis KCM1 | 83 | ND | ND | |
| X. laevis KIF2 | ND | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340
' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' '
CfDSK1 TNSNICIAVRKRPISDKERQKLDHDSVSCFQNKVWIHSAKLKVDGITKYLTHNSFQLDHTFGEDSTTEQIYLATTLPLVDHVVSTQGRATVFCYGQTGSGKTYTMNG---------IQQILAYDLYGQ-LAEHTDD-----LEITVAFFELYSGNVLDLLHGCQ-RCKLLEDGNGEVNITGLREVPAPTPEAFLQVIEEGHSLRTTQKTEANDASSRSHAICQVFLRDY-GGNLRGKLGLVDLAGSERGSDTKQHNSQRRTESADINTSLLALKECIRALGQKSAHVPYRGSKLTLILKDCF-SPDSKTTMVATVSPGASAADHSLNTLRYADRIKEQRVSSNGQ CfDSK1
DmKlp10A DDHQITVCVRKRPISRKEVNRKEIDVISVPRKDMLIVHEPRSKVD-LTKFLENHKFRFDYAFNDTCDNAMVYKYTAKPLVKTIFEGGMATCFAYGQTGSGKTHTMGGEFNGKVQDCKNGIYAMAAKDVFVTLNMPRYRAMNLVVSASFFEIYSGKVFDLLSDKQ-KLRVLEDGKQQVQVVGLTEKVVDGVEEVLKLIQHGNAARTSGQTSANSNSSRSHAVFQIVLRPQGSTKIHGKFSFIDLAGNERGVDTSSADRQTRMEGAEINKSLLALKECIRALGKQSAHLPFRVSKLTQVLRDSFIGEKSKTCMIAMISPGLSSCEHTLNTLRYADRVKELVVKDIVE DmKlp10A
DmKlp59C NCHQIMVCVRKRPLRRKELADREQDVVSIPSKHTLVVHEPRKHVN-LVKFLENHSFRFDYVFDEECSNATVYEFTARPLIKHIFDGGMATCFAYGQTGSGKTYTMGGQFPGRHQSSMDGIYAMAAKDVFSTLKTVPYNKLNLKVYCSFFEIYGTRVFDLLMPGKPQLRVLEDRNQQVQVVGLTQNPVQNTAEVLDLLELGNSVRTSGHTSANSKSSRSHAVFQIVLRSAAGEKLHGKFSLIDLAGNERGADNSSADRQTRLEGSEINKSLLVLKECIRALGRQSSHLPFRGSKLTQVLRDSFIGKKVKTCMIAMISPCLHSVEHTLNTLRYADRVKELSVESIPS DmKlp59C
HsKin2 DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTAKPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTAA HsKin2
HsMCAK EEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLNKKA-KLRVLEDGKQQVQVVGLQEHLVNSADDVIKMLDMGSACRTSGQTFANSNSSRSHACFQIILRAK--GRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPS HsMCAK
CeK11D9.c NENRISVCVRKRPLNKKELTKAEVDVITIPSRDITILHQPQTRVD-LTKYLDNQKFRFDYSFDEYANNELVYRFTAAPLVKTVFDNGTATCFAYGQTGSGKTHTMGGDFSGKKQNASMGIYALTARDVFRMLEQPQYRRKDLSVHCAFFEIYGTKTYDLLNDKA-ELRVLEDKMQKVQVVGLKEEQAHNEQDVLELINKGTLVRTAGTTSANANSSRSHAIFQIILRQG--KKVWGKFSLIDLAGNERGQDTRECDRDTRKEGANINTSLLALKECIRGMARNSSHVPFRQSKLTMVLRDSFIGEKSRTVMISMISPGISSSDHTLNTLRYADRVKEMGTDGSGE CeK11D9.c
LmKin RKSRILVAIRKRPLSAGEQTNGFTDIMDADNSGEIVLKEPKVKVD-LRKYTHVHRFFFDEVFDEACDNVDVYNRAARALIDTVFDGGCATCFAYGQTGSGKTHTMLGKGPEP------GLYALAAKDMFDRLTSD------TRIVVSFYEIYSGKLFDLLNGRR-PLRALEDDKGRVNIRGLTEHCSTSVEDLMTIIDQGSGVRSCGSTGANDTSSRSHAILEIKLKAKRTSKQSGKFTFIDLAGSERGADTVDCARQTRLEGAEINKSLLALKECIRFLDQNRKHVPFRGSKLTEVLRDSFIG-NCRTVMIGAVSPSNNNAEHTLNTLRYADRVKELKRNATER LmKin
CgMCAK EEHRICVCVRKRPLNKQELAKKEIDVISVPSKCLLFVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKSQNTSKGIYAMASRDVFLLKSQPRYRNLNLEVYVTFFEIYNGKVFDLLNKKA-KLRVLEDSKQQVQVVGLQEYLVNCADDVIKMLNMGSACRTSGQTFANSNSSRSHACFQILLRAK--GRLHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCEYTLNTLRYADRVKELSPHSGLS CgMCAK
MmKIF2 DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLRLKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKEFGISPSDI MmKIF2
MmKIF2C DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVNPAAA MmKIF2C
RnKRP2 EEHRICVCVRKRPLNKQELAKKEIDVISVPSKCLLLVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKSQNASKGIYAMASRDVFLLKNQPRYRSLNLEVYVTFFEIYNGKVFELLNKKA-KLRVLEDSKQQVQVVGLQEYLVTCADDVIKMINMGSACRTSGQTFANSNSSRSHACFQILLRAK--GRLHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKESIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCEYTLNTLRYADRVKELSPHSGPS RnKRP2
XlKCM1 EEHRICVCVRKRPLNKQELSKKEIDIISVPSKNIVLVHEPKLKVD-LTKYLENQAFRFDFSFDETATNEVVYRFTARPLVQSIFEGGKATCFAYGQTGSGKTHTMGGDFSGKSQNVSKGVYAFASRDVFLLLDQPRYKHLDLDVFVTFFEIYNGKVFDLLNKKT-KLRVLEDAKQEVQVVGLLEKQVISADDVFKMIEIGSACRTSGQTFANTSSSRSHACLQIILRRG--SKLHGKFSLVDLAGNERGVDTASADRITRMKGAEINRSLLALKECIRALGQNKSHTPFRESKLTQILRDSFIGENSRTCMIAMLSPGFNSCEYTLNTLRYADRVKELSPQNAET XlKCM1
XlKCM2 DEHRICVCVRKRPLNKKETTIKDLDVITIPIKDVVMVHEPKQKVD-LTRFLENQTFRFDYAFDETAPNETVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFQMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIEIGNSCRTSGQTSANAHSSRSHAVFQIILRKK--GKMHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELDPSRCRR XlKCM2
Go to the MT Disassembly page.
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 14 July 2006 2:31 GMT
