The Kinesin-5 Family

The Kinesin-5 (formerly BimC) family tree was obtained using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-5 proteins from humans and several model organisms. Kinesin-5 proteins from other organisms are also shown in the table and sequence alignment below.

Species/protein Molecular mass (kDa) Motor polarity & velocity Subcellular localization Comments
S. pombe Cut7 121 ND Spindle, poles Required for SPB separation and spindle formation
A. nidulans bimC 132 ND Functions in pole separation Essential for SPB separation in mitosis, mutants arrest with monoploar spindles
S. cerevisiae Kip1 126 ND Spindle, poles Redundant with Cin8
H. sapiens KIF8
S. cerevisiae Cin8 118 ND Spindle Required for separation of spindle poles, formation & maintenance of bipolar spindle
P. Falciparum MAL3P6.13 192
C. elegans F23B12.8 108 ND ND Fused with CeF08H9.1
H. sapiens KSP (HsEg5) 121 Plus, 2 µm/min ND Essential for centrosome separation in mitosis, mutants arrest with monopolar spindle
D. melanogaster Klp61F (KRP130) 121 (130) Plus, 2 µm/min ND Essential for centrosome separation in mitosis, mutants arrest with monopolar spindle, homotetramer
A. thaliana F16L2.60 119
A. thaliana KRP125a 116
A. thaliana KRP125b 119
A. thaliana KRP125c 120
B. mori Krp ND ND ND
N. tabacum KRP125 114 ND ND
X. laevis Eg5 119 Plus, 2 µm/min Spindle MTs Required for spindle formation in vitro at mitosis
X. laevis Eg52 120 ND Oocytes, eggs
          1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480                                
          '        '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '        '                                   

SpoCUT7   NETNINVVVRVRGRTDQEVRDNSSLAVSTSGAMGAELAIQS-----DPSSMLVTKTYAFDKVFGPEADQLMLFENSVAPMLEQVLNGYNCTIFAYGQTGTGKTYTMSGD-------LSDSDGILSEGAGLIPRALYQLFSSLDNSNQE-YAVKCSYYELYNEEIRDLLVSEE-------------------------------------------------------------------------------------------------------LRKPARVFEDTSRRGNVVITGIEESYIKNAGDGLRLLREGSHRRQVAATKCNDLSSRSHSIFTITLHRKVSSGMTDETNSLTINNNSDDLLRASKLHMVDLAGSENIGRSG----AENKRARETGMINQSLLTLGRVINALVEKAHHIPYRESKLTRLLQDSLGGKTKTSMIVTVSSTNTNLEETISTLEYAARAKSIRNKPQNN  SpoCUT7
AnBIMC    EDTSIHVVVRCRGRNEREVKENSGVVLQTEGVKGKTVELSM------GPNAVSNKTYTFDKVFSAAADQITVYEDVVLPIVTEMLAGYNCTIFAYGQTGTGKTYTMSGD-------MTDTLGILSDNAGIIPRVLYSLFAKLADTEST---VKCSFIELYNEELRDLLSAEE------------------------------------------------------------------------------------------------------NPKLKIYDNEQKKGHMSTLVQGMEETYIDSATAGIKLLQQGSHKRQVAATKCNDLSSRSHTVFTITVNIKR------------TTESGEEYVCPGKLNLVDLAGSENIGRSG----AENKRATEAGLINKSLLTLGRVINALVDKSQHIPYRESKLTRLLQDSLGGRTKTCIIATMSPARSNLEETISTLDYAFRAKNIRNKPQIN  AnBIMC
ScKIP1    SDSNIHVYVRCRSRNKREIEEKSSVVISTLGPQGKEIILSN---GSHQSYSSSKKTYQFDQVFGAESDQETVFNATAKNYIKEMLHGYNCTIFAYGQTGTGKTYTMSGDINILGDVQSTDNLLLGEHAGIIPRVLVDLFKELSSLNKE-YSVKISFLELYNENLKDLLSDSE-----------------------------------------------------------------------------------------------DDDPAVNDPKRQIRIFDNNNNNSSIMVKGMQEIFINSAHEGLNLLMQGSLKRKVAATKCNDLSSRSHTVFTITTNIVE--------QDSKDHGQNKNFVKIGKLNLVDLAGSENINRSG----AENKRAQEAGLINKSLLTLGRVINALVDHSNHIPYRESKLTRLLQDSLGGMTKTCIIATISPAKISMEETASTLEYATRAKSIKNTPQVN  ScKIP1
ScCIN8    EELNITVAVRCRGRNEREISMKSSVVVNVPDITGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQDLIFDEVAGPLFQDFIKGYNCTVLVYGMTSTGKTYTMTGD-------EKLYNGELSDAAGIIPRVLLKLFDTLELQQND-YVVKCSFIELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFASSTANNTTSNSASSSRSNSRNSSPRSLNDLTPKAALLRKRLRTKSLPNTIKQQYQQQQAVNSRNNSSSNSGSTTNNASSNTNTNNGQRSSMAPNDQTNGIYIQNLQEFHITNAMEGLNLLQKGLKHRQVASTKMNDFSSRSHTIFTITLYKKH----------------QDELFRISKMNLVDLAGSENINRSG----ALNQRAKEAGSINQSLLTLGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTALIATISPAKVTSEETCSTLEYASKAKNIKNKPQLG  ScCIN8
CeF23B12  PTSNLRVAVRIRPMNGTERSEKCTNVVKVDKGK-------------QAIELKGKSFGPFFRTYDPDTTQEEIYSDLVSSQIKKVIAGFNCTVFAYGQTGTGKTFTMEGG------RTDAKSSTDDPTTGIIPRAVEDIFEQLERCGCEEYSLRVSYIELYNEELFDLLASTD------------------------------------------------------------------------------------------------------NEDRERLRIFDDPNKKGVIVSGVEEVPVRNRSDVFKLLQLGAEKRRTAATLMNMHSSRSHSLFMVNVVIRE------------NTTTGEELVKQGKLNLVDLAGSENIGRSG----AQGNRAKEAGSINQSLLTLGRVIRLLTTNGQHIPYRESKLTRLLQDSLGGSTITSLIATLSPSSSNFEESQSTLEYAMRAANIKNKPVCN  CeF23B12
HsKSP     KGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTG-----GLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTE-FSVKVSLLEIYNEELFDLLNPSS------------------------------------------------------------------------------------------------------DVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKE------------TTIDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVN  HsKSP
DmKLP61F  SNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTL--------DSKLTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGN----ETAELKSSWEDDSDIGIIPRALSHLFDELRMMEVE-YTMRISYLELYNEELCDLLSTDD--------------------------------------------------------------------------------------------------------TTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRE------------NGIEGEDMLKIGKLNLVDLAGSENVSKAG---NEKGIRVRETVNINQSLLTLGRVITALVDRAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVN  DmKLP61F
BmKRP     KNQNIQVFVRLRPLNQRERDLKSLGVVEVHNNKEVVVRISQ--------QNSITKKFTFDRAFAPYANQVEVYQEVVSPLIEEVLAGYNCTVFAYGQTGTGKTHTMVGE-----NTGDETTWQKDPLAGIIPRALSQLFDELRISNTE-YTVRVSYLELYNEELFDLLATSE-------------------------------------------------------------------------------------------------------DNSKLRIYEDVTRKGSNIVNGLEEITVYNKKEVFRIMAQGQERKKVASTLMNAQSSRSHTVFTIVVHMKE-----------NSLPEGEELVKIGKLNLVDLAGSENISKAGSDNPAKRERARECVNINQSLLTLGRVITALVERHPHVPYRESKLTRILQESLGGRTKTSIIATISPGHKDLEETMSTLEYLTEQRTFRTSLRST  BmKRP
NtKRP125  KGVNVQVLLRCRPFSNDELRNNAPQVVTCNDYQREVAVSQN------IAGKHIDRIFTFDKVFGPSAQQRDLYDQAIVPIVNEVLEGFNCTIFAYGQTGTGKTYTMEGE---CKRSKSGPNGELPQEAGVIPRAVKQVFDTLESQNAE-YSVKVTFLELYNEEITDLLAPED-------------------------------------------------------------------------------------------------LKVALEDRQKKQLPLMEDGKGGVLVRGLEEEIVTSANEIFTLLERGSAKRRTAETLLNKQSSRSHSLFSITIHIKE------------ATPEGEELIKCGKLNLVDLAGSENISRSG----AREGRAREAGEINKSLLTLGRVINALVEHLGHIPYRDSKLTRLLRDSLGGRTKTCIIATVSPAVHCLEETLSTLDYAHRAKNIKNKPEVN  NtKRP125
XlEg5     KGKNIQVVVRCRPFNQLERKASSHSVLECDSQRKEVYVRTG-----EVNDKLGKKTYTFDMVFGPAAKQIEVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSSDEEFTWEQDPLAGIIPRTLHQIFEKLSENGTE-FSVKVSLLEIYNEELFDLLSPSP------------------------------------------------------------------------------------------------------DVGERLQMFDDPRNKRGVIIKGLEEISVHNKDEVYHILERGAAKRKTASTLMNAYSSRSHSVFSVTIHMKE------------TTVDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERAPHIPYRESKLTRILQDSLGGRTKTSIIATVSPASINLEETVSTLDYASRAKSIMNKPEVN  XlEg5
XlEg52    KGKNIQVVVRCRPFNQLERKASSHSVLECESQRKEVCVRTG-----EVNDKLGKKTYTFDMVFGPAAKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSSDEEFTWEQDPLAGIIPRTLHQIFEKLSEIGTE-FSVKVSLLEIYNEELFDLLSPSP------------------------------------------------------------------------------------------------------DVGERLQMFDDPRNKRGVIIKGLEEISVHNKDEVYQILERGAAKRKTASTLMNAYSSRSHSVFSVTIHMKE------------TTIDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERAPHIPYRESKLTRILQDSLGGRTKTSIIATVSPASINLEETMSTLDYASRAKNIMNKPEVN  XlEg52

If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.

Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:23 GMT