The Kinesin-1 (formerly KHC) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-1 proteins from humans and several model organisms. Kinesin-1 proteins from other organisms are also shown in the table and sequence alignment below.
|
Species/protein |
Molecular mass (kDa) |
Motor polarity & velocity |
Subcellular localization |
Comments |
|---|---|---|---|---|
| H. sapiens KHCx | 109 | ND | ND | |
| H. sapiens neuronal KHC | 117 | ND | Cell body in cultured neurons | Specific to neural tissue |
| H. sapiens KHC | 110 | ND | Uniform in cultured neurons | Ubiquitously expressed |
| D. melanogaster KHC | 110 | Plus, 54 µm/min | Diffuse in cytoplasm | Required for viability & neuromuscular function |
| C. elegans KHC (Unc-116) | 92 | ND | ND | Axonal transport of synaptic vesicles, also orients first mitotic spindle |
| D. discoideum K7 | ND | ND | ||
| L. pealii KHC | 109 | Plus, 30 µm/min | Membranous vesicles | |
| M. musculus KIF5a | ND | |||
| M. musculus KIF5b | ND | ND | Most similar to HsKHC (97% identity) | |
| M. musculus KIF5c | ND | ND | ||
| N. crassa KHC (NKin) | 103 | Plus, 120-180 µm/min | ND | High velocity of microtubule transport; no copurified light chains |
| N. haematocca Kin1 | 103 | ND | ND | |
| S. pombe Klp3 | ND | ND | ||
| S. purpuratus KHC | 118 | ND | ND | |
| S. racemosum SyKin1 | 105 | ND | ND | |
| U. maydis Kin2 | 107 | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340
' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' '
HsxKHC AECSIKVMCRFRPLNEAEILRGDKFIPKFK---GDETVVIG------QGKPYVFDRVLPPNTTQEQVYNACAKQIVKDVLEGYNGTIFAYGQTSSGKTHTMEGK-LHDPQLMGIIPRIAHDIFDHIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKTHVPYRDSKMTRILQDSLGGNCRTTIVICCSPSVFNEAETKSTLMFGQRAKTIKNTVSVN HsxK
HsnKHC NECSIKVLCRFRPLNQAEILRGDKFIPIFQ---GDDSVVIG-------GKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGK-LHDPQLMGIIPRIARDIFNHIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVN HsnKHC
HsKHC AECNIKVMCRFRPLNESEVNRGDKYIAKFQ---GEDTVVIA-------SKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGK-LHDPEGMGIIPRIVQDIFNYIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGS-TYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVN HsKHC
DmKHC AEDSIKVVCRFRPLNDSEEKAGSKFVVKFPNNVEENCISIA-------GKVYLFDKVFKPNASQEKVYNEAAKSIVTDVLAGYNGTIFAYGQTSSGKTHTMEGV-IGDSVKQGIIPRIVNDIFNHIYAMEVNLE-----FHIKVSYYEIYMDKIRDLLDVSKVNLSVHEDKNRVPYVKGATERFVSSPEDVFEVIEEGKSNRHIAVTNMNEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKTHIPYRDSKLTRILQESLGGNARTTIVICCSPASFNESETKSTLDFGRRAKTVKNVVCVN DmKHC
CeKHC AECGVQVFCRIRPLNKTEEKNADRFLPKFP---SEDSISLG-------GKVYVFDKVFKPNTTQEQVYKGAAYHIVQDVLSGYNGTVFAYGQTSSGKTHTMEGV-IGDNGLSGIIPRIVADIFNHIYSMDENLQ-----FHIKVSYYEIYNEKIRDLLDPEKVNLSIHEDKNRVPYVKGATERFVGGPDEVLQAIEDGKSNRMVAVTNMNEHSSRSHSVFLITVKQEHQTTKKQLTGKLYLVDLAGSEKVSKTGAQGTVLEEAKNINKSLTALGIVISALAEGTKSHVPYRDSKLTRILQESLGGNSRTTVIICASPSHFNEAETKSTLLFGARAKTIKNVVQIN CeKHC
DdK7 VSSNIRVVCRVRPLTELEKGRNEHSIVHFF---DSKSISIR-----ANGPQFTFDRIFGYQETQSQIFEDVAEPIVNDFLDGYHGTIIAYGQTASGKTFTMVGD----PDSHGIIPRVIESIFVGISKMREKDTSLSLAFCLKISALELYNEKLYDLYIASKSNLNIREHKQNGIYVEGISEIVITSIEEAYNFLNISNNNRAIASTKMSAASSRSHSVLMIELSQQNLSMESSKISKLFLVDLAGSDISHKTGAEGDRMQEAKNINLSLSALGKVINALTCGV-NYVPYRDSKLTRVLQDSLGGNSKTSLIINCSPSNNNEHETITTLQFGTRAKTIKNQPKIN DdK7
LpKHC SECNIKVICRVRPLNEAEERAGSKFILKFP---TDDSISIA-------GKVFVFDKVLKPNVSQEYVYNVGAKPIVADVLSGCNGTIFAYGQTSSGKTHTMEGV-LDKPSMHGIIPRIVQDIFNYIYGMDENLE-----FHIKISYYEIYLDKIRDLLDVTKTNLAVHEDKNRVPFVKGATERFVSSPEEVMEVIDEGKNNRHVAVTNMNEHSSRSHSVFLINVKQENVETQKKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALADGNKSHVPYRDSKLTRILQESLGGNARTTMVICCSPASYNESETKSTLLFGQRAKTIKNVVSVN LpKHC
MmKIF5a NECSIKVLCRFRPLNQAEILRGDKFIPIFQ---GDDSVIIG-------GKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGK-LHDPQLMGIIPRIARDIFNHIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENVETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDTKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVN MmKIF5a
MmKIF5b AECNIKVMCRFRPLNESEVNRGDKYVAKFQ---GEDTVVIA-------SKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGK-LHDPEGMGIIPRIVQDIFNYIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGS-TYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVN MmKIF5b
MmKIF5c AECSIKVMCRFRPLNEAEILRGDKFIPKFK---GEETVVIG------QGKPYVFDRVLPPNTTQEQVYNACAKQIVKDVLEGYNGTIFAYGQTSSGKTHTMEGK-LHDPQLMGIIPRIAHDIFDHIYSMDENLE-----FHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKTHVPYRDSKMTRILQDSLGGNCRTTIVICCSPSVFNEAETKSTLMFGQRAKTIKNTVSVN MmKIF5c
NcKHC SANSIKVVARFRPQNRVEIESGGQPIVTFQ---GPDTCTVD---SKEAQGSFTFDRVFDMSCKQSDIFDFSIKPTVDDILNGYNGTVFAYGQTGAGKSYTMMGTSIDDPDGRGVIPRIVEQIFTSILSSAANIE-----YTVRVSYMEIYMERIRDLLAPQNDNLPVHEEKNRGVYVKGLLEIYVSSVQEVYEVMRRGGNARAVAATNMNQESSRSHSIFVITITQKNVETGSAKSGQLFLVDLAGSEKVGKTGASGQTLEEAKKINKSLSALGMVINALTDGKSSHVPYRDSKLTRILQESLGGNSRTTLIINCSPSSYNDAETLSTLRFGMRAKSIKNKAKVN NcKHC
NhKin1 SANSIKVVARFRPQNKVELASGGMPIVSFD---GEDTCSLD---SKEAQGSFTFDRVFDMACKQQDIFDFSIRSTVDDILNGYNGTVFAYGQTGAGKSYTMMGTNIDDDDGRGVIPRIVEQIFASIMSSPGTIE-----YTVRVSYMEIYMERIRDLLAPQNDNLPVHEEKNRGVYVKGLLEIYVSSVQEVYEVMRRGGNARAVAATNMNQESSRSHSIFVITITQKNVETGSAKSGQLFLVDLAGSEKVGKTGASGQTLEEAKKINKSLSALGMVINALTDGKSSHIPYRDSKLTRILQESLGGNSRTTLIINCSPSSYNDAETLSTLRFGLRAKSIKNKAKVN NhKin1
SpoKlp3 -MTSIKVVCRIRPTNQLEQDLGGNNVIYPL--NDSTVHIET----SDYSGNFVFDRVFHPSSTLNDIFSYSIESTVDDLFLGYNGTVLAYGQTGSGKTYTMMGI-ENNFEKEGMTPRMLRRIFDKIRDSPSTTE-----YEVKVSYMEIYMEKIHDLLSEKNDRLTVHEDKLQGVYVQGLKTIYVSSETEALDILNKGMGSRAVASTSMNAQSSRSHSIFVLEVVQTDTESGETRRGRLFLVDLAGSESVGKSGAVGQTLEEAKKINRSLSTLGMVINSLTDSKLSHVPYRDSKLTRILKESLGGNSRTTLIINCSPDSYNATETLSTLRFGHRAKSIKNKAVVN SpoKlp3
SpKHC AECNIKVVCRVRPMNATEQNTSHICTKFI----SEEQVQIG-------GKLNMFDRIFKPNTTQEEVYNKAARQIVKDVLDGYNGTIFAYGQTSSGKTFTMEGV-MGNPQYMGIIPRIVQDIFNHIYQMDESLE-----FHIKVSYFEIYMDRIRDLLDVSKTNLSVHEDKNRVPFVKGATERFASSPEEVMDVIEEGKSNRHIAVTNMNEHSSRSHSIFLIQVKQENMETKKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGKKSHIPYRDSKMTRILQESLGGNARTTIVICCSPSSFNESESKSTLMFGQRAKTIKNTVTVN SpKHC
SyKin1 SGNNIKVVCRFRPQNSLEIREGGTPIIDID-PEGTQLELKG----KEFKGNFNFDKVFGMNTAQKDVFDYSIKTIVDDVTAGYNGTVFAYGQTGSGKTFTMMGADIDDEKTKGIIPRIVEQIFDSIMASPSNLE-----FTVKVSYMEIYMEKVRDLLNPSSENLPIHEDKTKGVYVKGLLEVYVGSTDEVYEVMRRGSNNRVVAYTNMNAESSRSHSIVMFTITQKNVDTGAAKSGKLYLVDLAGSEKVGKTGASGQTLEEAKKINKSLTALGMVINALTDGKSSHVPYRDSKLTRILQESLGGNSRTTLIINCSPSSYNEAETLSTLRFGARAKSIKNKAKVN SyKin1
UmKin2 MSNNIKVVCRFRPPNAIEQREGSDIVVDFSDDGSLVKLTRGVSTSGPEAGGFVFDKVFPMNTMQRDVFEFGIKETVEDVLNGYNGTIFAYGQTGSGKTFTMMGSDIDNDNLKGIIPRITEQIFENIMASPPHLE-----YLVKVSYMEIYMEKIRDLLAPQNDNLQVHEEKNRGVYVKGLSDFYVGGQADVYEIMRQGGLARAVSSTNMNAESSRSHSIFLITIQQRNTETGSAKTGNLYLVDLAGSEKVGKTGASGQTLEEAKKINKSLSALGMVINALTDGKSSHIPYRDSKLTRILQESLGGNSRTTLIVNASPCVYNADETLSTLRFGVRAKSIKNKARVN UmKin2
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:19 GMT
