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Construction of Plasmids |
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Mutants, Chimeras and Truncated Motors. |
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Expression and Purification of Kinesin Motors |
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Bacterial Expression of Motors |
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Purification of Ncd Motor Domain Protein |
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Purification of KHC Motor Domain Protein |
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Purification of Kar3 Motor Domain Protein |
| ATPase Assays |
|
Steady-State ATPase Assays |
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Single Turnover ATPase Assays |
|
ATP-Binding/Hydrolysis Assays |
| Microtubule Binding Assays |
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Pelleting Assays |
| Motility Assays |
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Motility using VE-DIC Microscopy |
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Motility with Fluorescent MTs |
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Motor Polarity using Fluorescent MTs |
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Motor Polarity with Axoneme-MTs |
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Single Motor Assays |
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Landing Assays |
| Reagents |
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Tubulin Preparation |
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Microtubule Assembly |
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Axoneme Preparation |
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Axoneme-microtubule Preparation |
| GPP As A Tag |
|
Single Motor Assay |
|
In Vivo Studies |
| Crystallization |
|
Crystallization Trials |
|
Motor Crystalization Conditions |
Return to the Kinesin Home Page.
Created 2 March 1999 21:00 GMT
Modified 4 December 1999 21:15 GMT
