Publications

Click here for full list of publications on PubMed.

*equal authorship contribution; corresponding author

  1. Wingler LM, Feld AP. (2021) Nanobodies as probes and modulators of cardiovascular GPCRs. J. Cardiovasc. Pharmacol. In press. [PMID 34840268]
  2. *Wellner A, *McMahon C, Gilman MSA, Clements JR, Clark S, Nguyen KM, Ho MH, Hu VJ, Shin J, Feldman J, Hauser BM, Caradonna TM, Wingler LM, Schmidt AG, Marks DS, Abraham J, Kruse AC, Liu CC. (2021) Rapid generation of potent antibodies by autonomous hypermutation in yeast. Nat. Chem. Biol. 17:1057-1064. [PMID 34168368]
  3. *McMahon C, *Staus DP, *Wingler LM, Wang J, Skiba MA, Elgeti M, Hubbell WL, Rockman HA, Kruse AC, Lefkowitz RJ. (2020) Synthetic nanobodies as angiotensin receptor blockers, Proc. Natl. Acad. Sci. USA 117:20284-20291. [PMID 32753386]
  4. Wingler LM, Lefkowitz RJ. (2020) Conformational basis of G protein-coupled receptor signaling versatility, Trends Cell Biol. 30:736-747. [PMID 32622699]
  5. Manglik A, Wingler LM, Rockman HA, Lefkowitz RJ. (2020) β-Arrestin-biased angiotensin II receptor agonists for COVID-19. Circulation 142:318–320. [PMID 32412788
  6. *Wingler LM, *Skiba MA, McMahon C, Staus DP, Kleinhenz ALW, Suomivuori C-M, Latorraca NR, Dror RO, Lefkowitz RJ, Kruse AC. (2020) Angiotensin II and biased analogs induce structurally distinct active conformations within a GPCR. Science 367:888-892. [PMID 32079768]
  7. *Suomivuori C-M, *Latorraca NR, Wingler LM, Eismann S, King MC, Kleinhenz ALW, Skiba MA, Staus DP, Kruse AC, Lefkowitz RJ, Dror RO. (2020) Molecular mechanism for biased signaling in a prototypical G-protein-coupled receptor. Science 367:881-887. [PMID 32079767]
  8. *Staus DP, *Hu H, *Robertson MJ, Kleinhenz ALW, Wingler LM, Capel WD, Latorraca NR, Lefkowitz RJ, Skiniotis G. (2020) Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc. Nature 579:297-302. [PMID 31945772]
  9. *Staus DP, *Wingler LM, *Pichugin D, Prosser RS, Lefkowitz RJ. (2019) Detergent- and phospholipid-based reconstitution systems have differential effects on constitutive activity of G protein-coupled receptors. J. Biol. Chem. 294:13218-13223. [PMID 31362983]
  10. *Wingler LM, *Elgeti M, *Hilger D, Latorraca NR, Lerch MT, Staus DP, Dror RO, Kobilka BK, Hubbell WL, Lefkowitz RJ. (2019) Angiotensin analogs with divergent bias stabilize distinct receptor conformations. Cell 176:468-478. [PMID 30639099Featured by Duke news service.
  11. *Wingler LM, *McMahon C, *Staus DP, Lefkowitz RJ, Kruse AC. (2019) Distinctive activation mechanism for angiotensin receptor revealed by a synthetic nanobody. Cell 176:479-490. [PMID 30639100Featured by Duke and Harvard news services.
  12. *Choi M, *Staus DP, Wingler LM, Ahn S, Pani B, Capel WD, Lefkowitz RJ. (2018) G protein-coupled receptor kinases (GRKs) orchestrate biased agonism at the β2-adrenergic receptor. Sci. Signal. 11:eaar7084. [PMID 30131371]
  13. *Staus DP, *Wingler LM, Choi M, Pani B, Manglik A, Kruse AC, Lefkowitz RJ. (2018) Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling. Proc. Natl. Acad. Sci. USA 115:3834-3839. [PMID 29581292]
  14. Ahn S, Pani B, Kahsai AW, Olsen EK, Husemoen G, Vestergaard M, Jin L, Zhao S, Wingler LM, Rambarat PK, Simhal RK, Xu TT, Sun LD, Shim PJ, Staus DP, Huang LY, Franch T, Chen X, Lefkowitz RJ. (2018) Small-molecule positive allosteric modulators of the β2-adrenoceptor isolated from DNA-encoded libraries. Mol. Pharmacol. 94:850-861. [PMID 29769246]
  15. Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC. (2017) Multidimensional tracking of GPCR signaling via peroxidase-catalyzed proximity labeling. Cell 169:338-349. [PMID 28388415]
  16. Ahn S, Kahsai AW, Pani B, Wang QT, Zhao S, Wall AL, Strachan RT, Staus DP, Wingler LM, Sun LD, Sinnaeve J, Choi M, Cho T, Xu TT, Hansen GM, Burnett MB, Lamerdin JE, Bassoni DL, Gavino BJ, Husemoen G, Olsen EK, Franch T, Costanzi S, Chen X, Lefkowitz RJ. (2017) Allosteric “beta-blocker” isolated from a DNA-encoded small molecule library. Proc. Natl. Acad. Sci. USA 114:1708-1713. [PMID 28130548]
  17. Kahsai AW, Wisler JW, Lee J, Ahn S, Cahill Iii TJ, Dennison SM, Staus DP, Thomsen AR, Anasti KM, Pani B, Wingler LM, Desai H, Bompiani KM, Strachan RT, Qin X, Alam SM, Sullenger BA, Lefkowitz RJ. (2016) Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor. Nat. Chem. Biol. 12:709-716. [PMID 27398998]
  18. Staus DP, Strachan RT, Manglik A, Pani B, Kahsai AW, Kim TH, Wingler LM, Ahn S, Chatterjee A, Masoudi A, Kruse AC, Pardon E, Steyaert J, Weis WI, Prosser RS, Kobilka BK, Costa T, Lefkowitz RJ. (2016) Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation. Nature 535:448-452. [PMID 27409812]
  19. Staus DP, Wingler LM, Strachan RT, Rasmussen SG, Pardon E, Ahn S, Steyaert J, Kobilka BK, Lefkowitz RJ. (2014) Regulation of beta-2-adrenergic receptor function by conformationally selective single-domain intrabodies. Mol. Pharmacol. 85:472-481. [PMID 24319111]
  20. Aristotelous T, Ahn S, Shukla AK, Gawron S, Sassano MF, Kahsai AW, Wingler LM, Zhu X, Tripathi-Shukla P, Huang XP, Riley J, Besnard J, Read KD, Roth BL, Gilbert IH, Hopkins AL, Lefkowitz RJ, Navratilova I. (2013) Discovery of beta 2 adrenergic receptor ligands using biosensor fragment screening of tagged wild-type receptor. ACS Med. Chem. Lett. 4:1005-1010. [PMID 24454993]
  21. Harton MH, Wingler LM, Cornish VW. (2013) Transcriptional regulation improves the throughput of three-hybrid counter selections in Saccharomyces cerevisiae. Biotechnol. J.  8:1485-1491 (2013). [PMID 24318638]
  22. Ostrov N, Wingler LM, Cornish VW. (2013) Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol. Biol. 978:187-203. [PMID 23423898]
  23. Wingler LM, Cornish VW. (2011) A library approach for the discovery of customized yeast three-hybrid counter selections. ChemBioChem 12:715-717. [PMID 21404416]
  24. Wingler LM, Cornish VW. (2011) Reiterative recombination for the in vivo assembly of libraries of multi-gene pathways. Proc. Natl. Acad. Sci. USA 108:15135-15140. [PMID 21876185].