Publications

2021

  • Perlatti B, Lan N, Xiang M, Earp CE, Spraker JE, Harvey CJB, Nichols CB, Alspaugh JA, Gloer JB, Bills GF. Anti-cryptococcal activity of preussolides A and B, phosphoethanolamine-substituted 24-membered macrolides, and leptosin C from coprophilous isolates of Preussia typharum. J Ind Microbiol Biotechnol. 2021 Dec 23;48(9-10):kuab022.DOI: 10.1093/jimb/kuab022
  • Telzrow CL, Zwack PJ, Esher Righi S, Dietrich FS, Chan C, Owzar K, Alspaugh JA, Granek JA. Comparative analysis of RNA enrichment methods for preparation of Cryptococcus neoformans RNA sequencing libraries. G3 (Bethesda). 2021 Oct 19;11(11):jkab301. DOI: 10.1093/g3journal/jkab301

2020

  • Chhetri, A, Loksztejn A, Nguyen H, Pianalto KM, Kim MJ, Hong J, Alspaugh JA,  Yokoyama K. Length Specificity and Polymerization Mechanism of (1,3)-β-d-Glucan Synthase in Fungal Cell Wall Biosynthesis. Biochemistry 2020 Feb 11;59(5):682-693. https://doi.org/10.1021/acs.biochem.9b00896.
  • Perlatti B, Harris G, Nichols CB, Ekanavake DI, Alspaugh J, Gloer JB, Bills GF. Campafungins: Inhibitors of Candida albicansand Cryptococcus neoformans Hyphal Growth. J Nat Prod. 2020;83(9):2718-2726. DOI: 10.1021/acs.jnatprod.0c00641
  • Perlatti B, Nichols CB, Alspaugh JA, Gloer JB, Bills GF. Sphaerostilbellins, New Antimicrobial Aminolipopeptide Peptaibiotics from Sphaerostilbella toxica. Biomolecules. 2020;10(10):1371. Published 2020 Sep 26. DOI: 10.3390/biom10101371
  • Oliveira FFM, Paes HC, Peconick LDF, Fonseca FL, Marina CLF, Bocca AL, Homem-de-Mello M, Rodrigues ML, Albuquerque P, Nicola AM, Alspaugh JA, Felipe MSS, Fernandes L. Erg6 affects membrane composition and virulence of the human fungal pathogen Cryptococcus neoformans. Fungal Genet Biol. 2020 Jul;140:103368.  DOI: 10.1016/j.fgb.2020.103368
  • Perlatti B, Nichols CB, Lan N, Wiemann P, Harvey CJB, Alspaugh JA, Bills GF. Identification of the Antifungal Metabolite Chaetoglobosin P From Discosia rubi Using a Cryptococcus neoformans Inhibition Assay: Insights Into Mode of Action and Biosynthesis. Front Microbiol. 2020 Jul 28;11:1766. DOI: 10.3389/fmicb.2020.01766
  • Brown HE, Telzrow CL, Saelens JW, Fernandes L, Alspaugh JA. Sterol-Response Pathways Mediate Alkaline Survival in Diverse Fungi. mBio. 2020;11(3):e00719-20. Published 2020 Jun 16. doi:10.1128/mBio.00719-20
  • Gusa A, Williams JD, Cho JE, Averette AF, Sun S, Shouse EM, Heitman J, Alspaugh JA, Jinks-Robertson S. Transposon mobilization in the human fungal pathogen Cryptococcus is mutagenic during infection and promotes drug resistance in vitro. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9973-9980. DOI: 10.1073/pnas.2001451117
  • Trevijano-Contador N, Pianalto KM, Nichols CB, Zaragoza O, Alspaugh JA, Pirofski LA. Human IgM Inhibits the Formation of Titan-Like Cells in Cryptococcus neoformans. Infect Immun. 2020 Mar 23;88(4):e00046-20. DOI: 10.1128/IAI.00046-20

 

2019

  • Telzrow, CL, Nichols, CB, Castro-Lopez, N, Wormley Jr., FL, Alspaugh, JA. A Fungal Arrestin Protein Contributes to Cell Cycle Progression and Pathogenesis. mBio Nov 2019, 10 (6) e02682-19; DOI: 10.1128/mBio.02682-19

  • Pianalto, KM Billmyre, BR, Telzrow, CL and Alspaugh, JA. Roles for Stress Response and Cell Wall Biosynthesis Pathways in Caspofungin Tolerance in Cryptococcus neoformans. GENETICS September 1, 2019 vol. 213 no. 1 213-227; https://doi.org/10.1534/genetics.119.302290
  • Dischler NM, Xu L, Li Y, Nichols CB, Alspaugh JA, Bills GF, Gloer JB. Wortmannin and Wortmannine Analogues from an Undescribed Niesslia sp. J Nat Prod. 2019;82(3):532-538. DOI: 10.1021/acs.jnatprod.8b00923

2018

  • Brown, HE, Ost, KS, Esher, SK, Pianalto, KM, Saelens, JW, Guan, Z, Alspaugh, JA. Identifying a novel connection between the fungal plasma membrane and pH sensing. Molecular Microbiology. June 8th. DOI: doi.org/10.1111/mmi.13998. 
  • Esher, SK, Ost, KS, Kohlbrenner, MA, Pianalto, KM, Telzrow, CL, Campuzano, A, Nichols, CB, Munro, C, Wormley, FL Jr., Alspaugh, JA. Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition. PLOS Path. June 4. DOI: 10.1371/journal.ppat.1007126. PubMed PMID:29864141.
  • Pianalto, KM, Ost, KS, Brown, HE, Alspaugh, JA. Characterization of additional components of the environmental pH-sensing complex in the pathogenic fungus Cryptococcus neoformans. JBC. May 16. DOI: 10.1074/jbc.RA118.002741. PMID:29769315.
  • Brandão F, Esher SK, Ost KS, Pianalto KP, Nichols CB, Fernandes L, Bocca A, Poças-Fonseca M, Alspaugh JA. HDAC genes play distinct and redundant roles in Cryptococcus neoformans virulence. Scientific Reports 2018 March 26;8(1):5209. DOI: 10.1038/s41598-018-21965-y. PubMed PMID:29581526.

2017

  • Ost KS, Esher SK, Leopold Wager CM, Walker L, Wagener J, Wormley FL Jr, and Alspaugh JA. Rim Pathway-Mediated Alterations in the Fungal Cell Wall Influence Immune Recognition and Inflammation. mBio 2017 Jan 31;8(1). DOI: 10.1128/mBio.02290-16. PubMed PMID:28143983.

2016

  • Esher SK, Ost KS, Kozubowski L, Yang D-H, Kim MS, Bahn Y-S, Alspaugh JA, Nichols CB. Relative Contribution of Prenylation and Postprenylation Processing in Cryptococcus neoformans Pathogenesis. mSphere 2016 March 30;1(2). DOI: 10.1128/mSphere.00084-15. PMID:27303728.

  • Pianalto K and Alspaugh JA. New Horizons in Antifungal Therapy. J. Fungi 2016 Oct 2;2(4). DOI: 10.3390/jof2040026. PubMed PMID:29376943.

2015

  • Esher SK, Granek JA, Alspaugh JA. Rapid mapping of insertional mutations to probe cell wall regulation in Cryptococcus neoformans. Fungal Genet Biol. 2015 Sep;82:9-21. DOI: 10.1016/j.fgb.2015.06.003. PubMed PMID: 26112692.
  • Nichols CB, Ost KS, Grogan DP, Pianalto K, Hasan S, Alspaugh JA. Impact of protein palmitoylation on the virulence potential of Cryptococcus neoformans. Eukaryot Cell. 2015 Jul;14(7):626-35. DOI: 10.1128/EC.00010-15. PubMed PMID: 25862155.
  • Ost KS, O’Meara TR, Huda N, Esher SK, Alspaugh JA. The Cryptococcus neoformans Alkaline Response Pathway: Identification of a Novel Rim Pathway Activator. PLoS Genet. 2015 Apr 10;11(4):e1005159. DOI: 10.1371/journal.pgen.1005159. PubMed PMID: 25859664.

2014

  • Lin X, Alspaugh JA, Liu H, Harris S. Fungal morphogenesis. Cold Spring Harb Perspect Med. 2014 Nov 3;5(2):a019679. DOI: 10.1101/cshperspect.a019679. PubMed PMID: 25367976.
  • Alspaugh JA. Virulence mechanisms and Cryptococcus neoformans pathogenesis. Fungal Genet Biol. 2015 May;78:55-8. DOI: 10.1016/j.fgb.2014.09.004. PubMed PMID: 25256589.
  • de Gontijo FA, Pascon RC, Fernandes L, Machado J Jr, Alspaugh JA, Vallim MA. The role of the de novo pyrimidine biosynthetic pathway in Cryptococcus neoformans high temperature growth and virulence. Fungal Genet Biol. 2014 Sep;70:12-23. DOI: 10.1016/j.fgb.2014.06.003. PubMed PMID: 25011011.

2013

  • Alspaugh JA. Hostile takeover: fungal protein promotes host cell invasion. J Clin Invest. 2014 Jan;124(1):74-6. DOI: 10.1172/JCI73585. PubMed PMID: 24355916.
  • O’Meara TR, Xu W, Selvig KM, O’Meara MJ, Mitchell AP, Alspaugh JA. The Cryptococcus neoformans Rim101 transcription factor directly regulates genes required for adaptation to the host. Mol Cell Biol. 2014 Feb;34(4):673-84. DOI: 10.1128/MCB.01359-13. PubMed PMID: 24324006.
  • Selvig K, Ballou ER, Nichols CB, Alspaugh JA. Restricted substrate specificity for the geranylgeranyltransferase-I enzyme in Cryptococcus neoformans: implications for virulence. Eukaryot Cell. 2013 Nov;12(11):1462-71. DOI: 10.1128/EC.00193-13. PubMed PMID: 24014765.
  • Ballou ER, Kozubowski L, Nichols CB, Alspaugh JA. Ras1 acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans. PLoS Genet. 2013;9(8):e1003687. DOI: 10.1371/journal.pgen.1003687. PubMed PMID: 23950731.
  • Ballou ER, Selvig K, Narloch JL, Nichols CB, Alspaugh JA. Two Rac paralogs regulate polarized growth in the human fungal pathogen Cryptococcus neoformans. Fungal Genet Biol. 2013 Aug;57:58-75. DOI: 10.1016/j.fgb.2013.05.006. PubMed PMID: 23748012.
  • O’Meara TR, Holmer SM, Selvig K, Dietrich F, Alspaugh JA. Cryptococcus
    neoformans Rim101 is associated with cell wall remodeling and evasion of the host immune responses. MBio. 2013 Jan 15;4(1). DOI:
    10.1128/mBio.00522-12. PubMed PMID: 23322637.

2012

  • O’Meara TR, Alspaugh JA. The Cryptococcus neoformans capsule: a sword and a shield. Clin Microbiol Rev. 2012 Jul;25(3):387-408. DOI: 10.1128/CMR.00001-12. PubMed PMID: 22763631.
  • Shen G, Zhou E, Alspaugh JA, Wang P. Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans. Eukaryot Cell. 2012 Apr;11(4):471-81. DOI: 10.1128/EC.00011-12. PubMed PMID: 22327008.

2011

  • Selvig K, Alspaugh JA. pH Response Pathways in Fungi: Adapting to Host-derived and Environmental Signals. Mycobiology. 2011 Dec;39(4):249-56. DOI: 10.5941/MYCO.2011.39.4.249. PubMed PMID: 22783112.
  • Stout JE, Gadkowski LB, Rath S, Alspaugh JA, Miller MB, Cox GM.
    Pedicure-associated rapidly growing mycobacterial infection: an endemic disease. Clin Infect Dis. 2011 Oct;53(8):787-92. DOI: 10.1093/cid/cir539. PubMed PMID:21921222.
  •  Okagaki LH, Wang Y, Ballou ER, O’Meara TR, Bahn YS, Alspaugh JA, Xue C, Nielsen K. Cryptococcal titan cell formation is regulated by G-protein signaling in response to multiple stimuli. Eukaryot Cell. 2011 Oct;10(10):1306-16. DOI: 10.1128/EC.05179-11. PubMed PMID: 21821718.
  • Hast MA, Nichols CB, Armstrong SM, Kelly SM, Hellinga HW, Alspaugh JA, Beese LS. Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens. J Biol Chem. 2011 Oct 7;286(40):35149-62. DOI: 10.1074/jbc.M111.250506.
    PubMed PMID: 21816822.
  • Schell WA, O’Donnell K, Alspaugh JA. Heterothallic mating in Mucor irregularis and first isolate of the species outside of Asia. Med Mycol. 2011 Oct;49(7):714-23. DOI: 10.3109/13693786.2011.568975. Erratum in: Med Mycol. 2011 Oct;49(7):723. PubMed PMID: 21453223.

2010

  • O’Meara TR, Hay C, Price MS, Giles S, Alspaugh JA. Cryptococcus neoformans histone acetyltransferase Gcn5 regulates fungal adaptation to the host. Eukaryot Cell. 2010 Aug;9(8):1193-202. DOI: 10.1128/EC.00098-10. PubMed PMID: 20581290.
  • O’Meara TR, Norton D, Price MS, Hay C, Clements MF, Nichols CB, Alspaugh JA. Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. PLoS Pathog. 2010 Feb 19;6(2):e1000776. DOI: 10.1371/journal.ppat.1000776. PubMed PMID: 20174553.
  • Ballou ER, Nichols CB, Miglia KJ, Kozubowski L, Alspaugh JA. Two CDC42 paralogues modulate Cryptococcus neoformans thermotolerance and morphogenesisunder host physiological conditions. Mol Microbiol. 2010 Feb;75(3):763-80. DOI:
    10.1111/j.1365-2958.2009.07019.x. PubMed PMID: 20025659.

These and additional Alspaugh lab publications pre-2010 available here.