I start my day around 10-10:30am when I arrive at the lab. After settling in, my first task is to review my notes from the previous day. This helps me stay organized and recall the progress and outcomes of my ongoing experiments. Based on this information, I create a to-do list for the day, outlining the tasks I need to accomplish.
Next, I seek out my mentor, Samuel. Meeting with Samuel allows me to gain valuable insights, receive feedback on my work, and clarify any doubts or questions I may have.
Now to the real action in the lab – juggling multiple experiments with different timelines.
One of the experiments I’m currently working on involves mating and crossing strains of cells. In another area of the lab, I conduct transformation experiments. I manipulate the genetic makeup of cells by introducing foreign DNA, such as plasmids. I also perform spot assays. By spotting cells onto different plates, I observe growth patterns or changes in color. This assay enables us to identify potential phenotypes, assess antibiotic resistance, and delve deeper into various biological processes. A significant portion of my work also revolves around imaging. I prepare slides and chambers and do time course and time lapse imaging. These images provide valuable clues revealing complex systems at play.
Around 1:00 pm, I take a lunch break. Typically bringing my own lunch to the lab, I quickly heat it in the microwave and enjoy the break, often while conversing with other people in the lab. Rejuvenated and refocused after lunch, I dive back into the lab, determined to complete my tasks.
At the end of the lab day, I record any final notes. I say goodbye to everyone and leave the lab, knowing that I have made progress!
Great! Smart idea to review your previous notes at the start of the day. That way you’re primed and ready to continue the work!