An email from Julie,

Hi Jianying, 
Here is the info you need. There are 4 different samples, all were done in biological triplicate. All tissue is liver, taken from the left lobe.
LN = liquid nitrogen  - tissue frozen in liquid nitrogen 
PFPE_tissue = liver tissue fixed by paxgene method 
OCT_LCM = liver tissue frozen in OCT, sectioned and regions laser microdissected
PFPE_LCM = liver tissue fixed by Paxgene method, embedded in paraffin, sectioned and regions laser microdissected.

Different kits were used for RNA extraction of LN, PFPE/PFPE_LCM and OCT_LCM. 3 kits used. Isolations done on 3 different days.

Let me know if you need more information and thank you very much.
J.

Honestly, there is NOT much biology in the study, but really testing the sample preparation procedure. So, standard QC will help to answer it.