Shenoy Lab

Full List of Publications:  Shenoy SK PubMed Papers

Selected Publications:

• Kaur S, Chen Y, Shenoy SK. Agonist-activated glucagon receptors are deubiquitinated at early endosomes by two distinct deubiquitinases to facilitate Rab4a-dependent recycling. J Biol Chem. 2020 Dec 4;295(49):16630-16642. doi: 10.1074/jbc.RA120.014532. Epub 2020 Sep 23. PubMed PMID: 32967969; PubMed Central PMCID: PMC7864061.

• Nagi K, Kaur S, Bai Y, Shenoy SK. In-frame fusion of SUMO1 enhances βarrestin2’s association with activated GPCRs as well as with nuclear pore complexes. Cell Signal. 2020 Nov;75:109759. doi: 10.1016/j.cellsig.2020.109759. Epub 2020 Aug 27. PubMed PMID: 32860951.

• Ahn S, Shenoy SK, Luttrell LM, Lefkowitz RJ. SnapShot: β-Arrestin Functions. 2020 Sep 3;182(5):1362-1362.e1. doi: 10.1016/j.cell.2020.07.034. PubMed PMID: 32888497.

• Toth K, Nagi K, Slosky LM, Rochelle L, Ray C, Kaur S, Shenoy SK, Caron MG, Barak LS. Encoding the β-Arrestin Trafficking Fate of Ghrelin Receptor GHSR1a: C-Tail-Independent Molecular Determinants in GPCRs. ACS Pharmacol Transl Sci. 2019 Aug 9;2(4):230-246. doi: 10.1021/acsptsci.9b00018. eCollection 2019 Aug 9. PubMed PMID: 32259059; PubMed Central PMCID: PMC7088988.

• Yu SM, Jean-Charles PY, Abraham DM, Kaur S, Gareri C, Mao L, Rockman HA, Shenoy SK. The deubiquitinase ubiquitin-specific protease 20 is a positive modulator of myocardial β₁-adrenergic receptor expression and signaling. J Biol Chem. 2019 Feb 15;294(7):2500-2518. doi: 10.1074/jbc.RA118.004926. Epub 2018 Dec 11. PubMed PMID: 30538132; PubMed Central PMCID: PMC6378982.

• Nagi K, Shenoy SK. Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination Using BRET. Methods Mol Biol. 2019;1957:93-104. doi: 10.1007/978-1-4939-9158-7_6. PubMed PMID: 30919349.

• Zhang L, Wu JH, Huang TQ, Nepliouev I, Brian L, Zhang Z, Wertman V, Rudemiller NP, McMahon TJ, Shenoy SK, Miller FJ, Crowley SD, Freedman NJ, Stiber JA. Drebrin regulates angiotensin II-induced aortic remodelling. Cardiovasc Res. 2018 Nov 1;114(13):1806-1815. doi: 10.1093/cvr/cvy151. PubMed PMID: 29931051; PubMed Central PMCID: PMC6198746.

• Jean-Charles PY, Wu JH, Zhang L, Kaur S, Nepliouev I, Stiber JA, Brian L, Qi R, Wertman V, Shenoy SK, Freedman NJ. USP20 (Ubiquitin-Specific Protease 20) Inhibits TNF (Tumor Necrosis Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates Atherosclerosis. Arterioscler Thromb Vasc Biol. 2018 Oct;38(10):2295-2305. doi: 10.1161/ATVBAHA.118.311071. PubMed PMID: 30354204; PubMed Central PMCID: PMC6205742.

• Luttrell LM, Wang J, Plouffe B, Smith JS, Yamani L, Kaur S, Jean-Charles PY, Gauthier C, Lee MH, Pani B, Kim J, Ahn S, Rajagopal S, Reiter E, Bouvier M, Shenoy SK, Laporte SA, Rockman HA, Lefkowitz RJ. Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9. Sci Signal. 2018 Sep 25;11(549). doi: 10.1126/scisignal.aat7650. PubMed PMID: 30254056; PubMed Central PMCID: PMC6369040.

• Rajagopal S, Shenoy SK. GPCR desensitization: Acute and prolonged phases. Cell Signal. 2018 Jan;41:9-16. doi: 10.1016/j.cellsig.2017.01.024. Epub 2017 Jan 28. Review. PubMed PMID: 28137506; PubMed Central PMCID: PMC5533627.

• Freedman NJ, Shenoy SK. Regulation of inflammation by β-arrestins: Not just receptor tales. Cell Signal. 2018 Jan;41:41-45. doi: 10.1016/j.cellsig.2017.02.008. Epub 2017 Feb 9. Review. PubMed PMID: 28189586; PubMed Central PMCID: PMC5550347.

• Jean-Charles PY, Yu SM, Abraham D, Kommaddi RP, Mao L, Strachan RT, Zhang ZS, Bowles DE, Brian L, Stiber JA, Jones SN, Koch WJ, Rockman HA, Shenoy SK. Mdm2 regulates cardiac contractility by inhibiting GRK2-mediated desensitization of β-adrenergic receptor signaling. JCI Insight. 2017 Sep 7;2(17). doi: 10.1172/jci.insight.95998. eCollection 2017 Sep 7. PubMed PMID: 28878120; PubMed Central PMCID: PMC5621915.

• Jean-Charles PY, Kaur S, Shenoy SK. G Protein-Coupled Receptor Signaling Through β-Arrestin-Dependent Mechanisms. J Cardiovasc Pharmacol. 2017 Sep;70(3):142-158. doi: 10.1097/FJC.0000000000000482. Review. PubMed PMID: 28328745; PubMed Central PMCID: PMC5591062.

• Jean-Charles PY, Rajiv V, Shenoy SK. Ubiquitin-Related Roles of β-Arrestins in Endocytic Trafficking and Signal Transduction. J Cell Physiol. 2016 Oct;231(10):2071-80. doi: 10.1002/jcp.25317. Epub 2016 Feb 3. Review. PubMed PMID: 26790995.

• Feger BJ, Thompson JW, Dubois LG, Kommaddi RP, Foster MW, Mishra R, Shenoy SK, Shibata Y, Kidane YH, Moseley MA, Carnell LS, Bowles DE. Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection. Sci Rep. 2016 Sep 27;6:34091. doi: 10.1038/srep34091. PubMed PMID: 27670941; PubMed Central PMCID: PMC5037457.

• Jean-Charles PY, Freedman NJ, Shenoy SK. Chapter Nine – Cellular Roles of Beta-Arrestins as Substrates and Adaptors of Ubiquitination and Deubiquitination. Prog Mol Biol Transl Sci. 2016;141:339-69. doi: 10.1016/bs.pmbts.2016.04.003. Epub 2016 May 7. Review. PubMed PMID: 27378762.
• Jean-Charles PY, Zhang L, Wu JH, Han SO, Brian L, Freedman NJ, Shenoy SK. Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation. J Biol Chem. 2016 Apr 1;291(14):7450-64. doi: 10.1074/jbc.M115.687129. Epub 2016 Feb 2. PubMed PMID: 26839314; PubMed Central PMCID: PMC4817176.
• Jean-Charles PY, Snyder JC, Shenoy SK. Chapter One – Ubiquitination and Deubiquitination of G Protein-Coupled Receptors. Prog Mol Biol Transl Sci. 2016;141:1-55. doi: 10.1016/bs.pmbts.2016.05.001. Review. PubMed PMID: 27378754.

• Kommaddi RP, Jean-Charles PY, Shenoy SK. Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress. J Biol Chem. 2015 Apr 3;290(14):8888-903. doi: 10.1074/jbc.M114.630541. Epub 2015 Feb 9. PubMed PMID: 25666616; PubMed Central PMCID: PMC4423680.

• Shenoy SK. Deubiquitinases and their emerging roles in β-arrestin-mediated signaling. Methods Enzymol. 2014;535:351-70. doi: 10.1016/B978-0-12-397925-4.00020-1. PubMed PMID: 24377933.

• Shenoy SK. Arrestin interaction with E3 ubiquitin ligases and deubiquitinases: functional and therapeutic implications. Handb Exp Pharmacol. 2014;219:187-203. doi: 10.1007/978-3-642-41199-1_10. PubMed PMID: 24292831.

• Han SO, Kommaddi RP, Shenoy SK. Distinct roles for β-arrestin2 and arrestin-domain-containing proteins in β2 adrenergic receptor trafficking. EMBO Rep. 2013 Feb;14(2):164-71. doi: 10.1038/embor.2012.187. Epub 2012 Dec 4. PubMed PMID: 23208550; PubMed Central PMCID: PMC3596129.

• Kommaddi RP, Shenoy SK. Arrestins and protein ubiquitination. Prog Mol Biol Transl Sci. 2013;118:175-204. doi: 10.1016/B978-0-12-394440-5.00007-3. Review. PubMed PMID: 23764054.

• Han SO, Xiao K, Kim J, Wu JH, Wisler JW, Nakamura N, Freedman NJ, Shenoy SK. MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors. J Cell Biol. 2012 Nov 26;199(5):817-30. doi: 10.1083/jcb.201208192. Epub 2012 Nov 19. PubMed PMID: 23166351; PubMed Central PMCID: PMC3514787.

• Shenoy SK, Han S, Zhao YL, Hara MR, Oliver T, Cao Y, Dewhirst MW. β-arrestin1 mediates metastatic growth of breast cancer cells by facilitating HIF-1-dependent VEGF expression. 2012 Jan 19;31(3):282-92. doi: 10.1038/onc.2011.238. Epub 2011 Jun 20. PubMed PMID: 21685944; PubMed Central PMCID: PMC3179824.

• Schulte G, Shenoy SK. β-Arrestin and dishevelled coordinate biased signaling. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19839-40. doi: 10.1073/pnas.1117444108. Epub 2011 Nov 28. PubMed PMID: 22123954; PubMed Central PMCID: PMC3250156.

• Shenoy SK, Rockman HA. Cardiovascular biology: heart fails without pump partner. 2011 Sep 28;477(7366):546-7. doi: 10.1038/477546a. PubMed PMID: 21956327.

• Sarker S, Xiao K, Shenoy SK. A tale of two sites: How ubiquitination of a G protein-coupled receptor is coupled to its lysosomal trafficking from distinct receptor domains. Commun Integr Biol. 2011 Sep;4(5):528-31. doi: 10.4161/cib.4.5.16458. Epub 2011 Sep 1. PubMed PMID: 22046454; PubMed Central PMCID: PMC3204120.

• Shenoy SK, Lefkowitz RJ. β-Arrestin-mediated receptor trafficking and signal transduction. Trends Pharmacol Sci. 2011 Sep;32(9):521-33. doi: 10.1016/j.tips.2011.05.002. Epub 2011 Jun 15. Review. PubMed PMID: 21680031; PubMed Central PMCID: PMC3159699.

• Nobles KN, Xiao K, Ahn S, Shukla AK, Lam CM, Rajagopal S, Strachan RT, Huang TY, Bressler EA, Hara MR, Shenoy SK, Gygi SP, Lefkowitz RJ. Distinct phosphorylation sites on the β(2)-adrenergic receptor establish a barcode that encodes differential functions of β-arrestin. Sci Signal. 2011 Aug 9;4(185):ra51. doi: 10.1126/scisignal.2001707. PubMed PMID: 21868357; PubMed Central PMCID: PMC3415961.

• Xiao K, Shenoy SK. Beta2-adrenergic receptor lysosomal trafficking is regulated by ubiquitination of lysyl residues in two distinct receptor domains. J Biol Chem. 2011 Apr 8;286(14):12785-95. doi: 10.1074/jbc.M110.203091. Epub 2011 Feb 17. PubMed PMID: 21330366; PubMed Central PMCID: PMC3069478.

• Shenoy SK. Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy. Methods Mol Biol. 2011;756:333-42. doi: 10.1007/978-1-61779-160-4_20. PubMed PMID: 21870237.

• Shenoy SK. β-arrestin-biased signaling by the β-adrenergic receptors. Curr Top Membr. 2011;67:51-78. doi: 10.1016/B978-0-12-384921-2.00003-3. Review. PubMed PMID: 21771485.

• Berthouze M, Venkataramanan V, Li Y, Shenoy SK. The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization. EMBO J. 2009 Jun 17;28(12):1684-96. doi: 10.1038/emboj.2009.128. Epub 2009 May 7. PubMed PMID: 19424180; PubMed Central PMCID: PMC2699358.

• Shenoy SK, Modi AS, Shukla AK, Xiao K, Berthouze M, Ahn S, Wilkinson KD, Miller WE, Lefkowitz RJ. Beta-arrestin-dependent signaling and trafficking of 7-transmembrane receptors is reciprocally regulated by the deubiquitinase USP33 and the E3 ligase Mdm2. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6650-5. doi: 10.1073/pnas.0901083106. Epub 2009 Apr 10. PubMed PMID: 19363159; PubMed Central PMCID: PMC2667797.

• Shenoy SK, Xiao K, Venkataramanan V, Snyder PM, Freedman NJ, Weissman AM. Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor. J Biol Chem. 2008 Aug 8;283(32):22166-76. doi: 10.1074/jbc.M709668200. Epub 2008 Jun 10. PubMed PMID: 18544533; PubMed Central PMCID: PMC2494938.

• Shenoy SK, Barak LS, Xiao K, Ahn S, Berthouze M, Shukla AK, Luttrell LM, Lefkowitz RJ. Ubiquitination of beta-arrestin links seven-transmembrane receptor endocytosis and ERK activation. J Biol Chem. 2007 Oct 5;282(40):29549-62. doi: 10.1074/jbc.M700852200. Epub 2007 Jul 31. PubMed PMID: 17666399; PubMed Central PMCID: PMC2216744.

• Shenoy SK. Seven-transmembrane receptors and ubiquitination. Circ Res. 2007 Apr 27;100(8):1142-54. doi: 10.1161/01.RES.0000261939.88744.5a. Review. PubMed PMID: 17463329; PubMed Central PMCID: PMC1952537.

• Girnita L, Shenoy SK, Sehat B, Vasilcanu R, Vasilcanu D, Girnita A, Lefkowitz RJ, Larsson O. Beta-arrestin and Mdm2 mediate IGF-1 receptor-stimulated ERK activation and cell cycle progression. J Biol Chem. 2007 Apr 13;282(15):11329-38. doi: 10.1074/jbc.M611526200. Epub 2007 Feb 15. PubMed PMID: 17303558.

• DeWire SM, Ahn S, Lefkowitz RJ, Shenoy SK. Beta-arrestins and cell signaling. Annu Rev Physiol. 2007;69:483-510. doi: 10.1146/annurev.physiol.69.022405.154749. Review. PubMed PMID: 17305471.

• Drake MT, Shenoy SK, Lefkowitz RJ. Trafficking of G protein-coupled receptors. Circ Res. 2006 Sep 15;99(6):570-82. doi: 10.1161/01.RES.0000242563.47507.ce. PubMed PMID: 16973913.

• Shenoy SK, Drake MT, Nelson CD, Houtz DA, Xiao K, Madabushi S, Reiter E, Premont RT, Lichtarge O, Lefkowitz RJ. beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor. J Biol Chem. 2006 Jan 13;281(2):1261-73. doi: 10.1074/jbc.M506576200. Epub 2005 Nov 9. PubMed PMID: 16280323.

• Shenoy SK, Lefkowitz RJ. Receptor regulation: beta-arrestin moves up a notch. Nat Cell Biol. 2005 Dec;7(12):1159-61. doi: 10.1038/ncb1205-1059. PubMed PMID: 16319967.

• Girnita L, Shenoy SK, Sehat B, Vasilcanu R, Girnita A, Lefkowitz RJ, Larsson O. {beta}-Arrestin is crucial for ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as adaptor for the MDM2 E3 ligase. J Biol Chem. 2005 Jul 1;280(26):24412-9. doi: 10.1074/jbc.M501129200. Epub 2005 May 3. PubMed PMID: 15878855.

• Lefkowitz RJ, Shenoy SK. Transduction of receptor signals by beta-arrestins. 2005 Apr 22;308(5721):512-7. doi: 10.1126/science.1109237. Review. PubMed PMID: 15845844.
• Shenoy SK, Lefkowitz RJ. Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. J Biol Chem. 2005 Apr 15;280(15):15315-24. doi: 10.1074/jbc.M412418200. Epub 2005 Feb 7. PubMed PMID: 15699045.
• Shenoy SK, Lefkowitz RJ. Resonating to the music of ubiquitination. Nat Methods. 2004 Dec;1(3):191-3. doi: 10.1038/nmeth1204-191. PubMed PMID: 15782194.

• Ahn S, Shenoy SK, Wei H, Lefkowitz RJ. Differential kinetic and spatial patterns of beta-arrestin and G protein-mediated ERK activation by the angiotensin II receptor. J Biol Chem. 2004 Aug 20;279(34):35518-25. doi: 10.1074/jbc.M405878200. Epub 2004 Jun 17. PubMed PMID: 15205453.

• Martin NP, Lefkowitz RJ, Shenoy SK. Regulation of V2 vasopressin receptor degradation by agonist-promoted ubiquitination. J Biol Chem. 2003 Nov 14;278(46):45954-9. doi: 10.1074/jbc.M308285200. Epub 2003 Sep 5. PubMed PMID: 12960162.

• Shenoy SK, Lefkowitz RJ. Multifaceted roles of beta-arrestins in the regulation of seven-membrane-spanning receptor trafficking and signalling. Biochem J. 2003 Nov 1;375(Pt 3):503-15. doi: 10.1042/BJ20031076. Review. PubMed PMID: 12959637; PubMed Central PMCID: PMC1223736.

• Shenoy SK, Lefkowitz RJ. Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. J Biol Chem. 2003 Apr 18;278(16):14498-506. doi: 10.1074/jbc.M209626200. Epub 2003 Feb 6. PubMed PMID: 12574160.

• Shenoy SK, McDonald PH, Kohout TA, Lefkowitz RJ. Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin. 2001 Nov 9;294(5545):1307-13. doi: 10.1126/science.1063866. Epub 2001 Oct 4. PubMed PMID: 11588219.