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A Rapid and Sensitive Fluorescence Assay for Quantifying PET Hydrolysis by Ideonella sakaiensis Based on the Iron (II)-Catalyzed Radical Hydroxylation of Terephthalic Acid



Justin Yang, a Duke Scholar in Marine Medicine, worked on applying a new fluorescence assay towards measuring plastic degradation by bacteria. This new method is faster and simpler to use than conventional methods like HPLC-MS and GC-MS, therefore promising to greatly accelerate the discovery and characterization of plastic-degrading microbes.

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2 thoughts on “A Rapid and Sensitive Fluorescence Assay for Quantifying PET Hydrolysis by Ideonella sakaiensis Based on the Iron (II)-Catalyzed Radical Hydroxylation of Terephthalic Acid”

  1. Yoav Kargon

    Nice work! Are TPA or similar compounds common byproduct of plastic degradation? Could this assay or a similar technique be used to quantify the degradation of other plastics by other microbes?

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