This section will explain the general setup of any experiment when using Topspin. This tutorial covers dataset creation, sample insertion, locking, tuning, shimming, and automatic receiver gain.
Commands to be typed in blue; Commands to click on in copper.
Setup for nearly every type of experiment in Topspin:(1) Click on Start Tab ... at top of program (2) Click on Create Dataset button ... or type: edc (3) In new window, type the name of your dataset, set the experiment number (expno) and processing number (procno) ... typically these are "1"
(4) Click on Select button next to the "Experiment" line (5) In new window type: *zg*
(6) Select appropriate experiment: ZGPR ... or whatever experiment you want (7) Click on Set selected item in editor button
(8) Click on Set solvent radio button and select your solvent (9) Click on Execute 'getprosol' radio button (10) Optional: Add notes in the Title section (11) Click on OK button
(12) Click on Aquire Tab ... at top of program (13) Type: sx 1 ... where "1" is the sample position in the sample changer ... or Click on Sample button at top of page ... and type the sample position in new window (14) Type: lock ... or Click on Lock button at top of page (15) Click on the solvent of your sample ... CDCl3 ... or whichever solvent ... click on OK button
(16) Click on Tune button ... or type: atma ... for automatic tuning (17) Click on Shim button ... or type: topshim ... or ... topshim gui
(17.1) If you typed: topshim gui ... click on Start button ... default parameters are correct ... click on Report tab ... make sure the "final B0 stdDev" is less than 1 ... if not rerun topshim ... if complete, click on Shim tab and then click on Close button (18) Click on Prosol button ... or type: getprosol (19) Click on Gain button ... or type: rga
