2025
Jamieson PJ, Shen X, Abu-Shmais AA, Wasdin PT, Janowska K, Edwards RJ, et al. Glycan-reactive antibodies isolated from human HIV-1 vaccine trial participants show broad pathogen cross-reactivity. J Virol. 2025:e0125625. Epub 20251110. doi: 10.1128/jvi.01256-25. PubMed PMID: 41212034.
Liu Q, Parsons RJ, Wiehe K, Edwards RJ, Saunders KO, Zhang P, et al. Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Structure. 2025;33(8):1325-36 e5. Epub 20250523. doi: 10.1016/j.str.2025.04.020. PubMed PMID: 40412376; PubMed Central PMCID: PMC12335366.
Harris C, Kapingidza AB, San JE, Christopher J, Gavitt T, Rhodes B, et al. Design of SARS-CoV-2 RBD immunogens to focus immune responses toward conserved coronavirus epitopes. J Virol. 2025;99(7):e0046525. Epub 20250613. doi: 10.1128/jvi.00465-25. PubMed PMID: 40511920; PubMed Central PMCID: PMC12282168.
Malewana RD, Stalls V, May A, Lu X, Martinez DR, Schafer A, et al. Nonstabilized SARS-CoV-2 spike mRNA vaccination induces broadly neutralizing antibodies in nonhuman primates. Sci Transl Med. 2025;17(802):eadn5651. Epub 20250611. doi: 10.1126/scitranslmed.adn5651. PubMed PMID: 40498855.
Thakur B, Katte RH, Xu W, Janowska K, Sammour S, Henderson R, et al. Conformational trajectory of the HIV-1 fusion peptide during CD4-induced envelope opening. Nat Commun. 2025;16(1):4595. Epub 20250517. doi: 10.1038/s41467-025-59721-2. PubMed PMID: 40382314; PubMed Central PMCID: PMC12085566.
Langel SN, Otero CE, Steppe JT, Williams CA, Travieso T, Chang J, et al. Breast milk delivery of an engineered dimeric IgA protects neonates against rotavirus. Mucosal Immunol. 2025;18(2):441-52. Epub 20250120. doi: 10.1016/j.mucimm.2025.01.002. PubMed PMID: 39842610; PubMed Central PMCID: PMC11982437.
Swanson OM, Zhang QE, Van Itallie E, Tian M, Brown AR, Harris C, et al. An engineered immunogen activates diverse HIV broadly neutralizing antibody precursors and promotes acquisition of improbable mutations. Sci Transl Med. 2025;17(780):eadr2218. Epub 20250108. doi: 10.1126/scitranslmed.adr2218. PubMed PMID: 39772772.
Science Translational Medicine; Volume 17; Issue 780; 8 Jan 2025; Swanson et al.
.
2024
Trimarco JD, Spurrier MA, Skavicus S, Luo Z, Dutta M, Janowska K, et al. Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions. J Virol. 2024;98(11):e0138524. Epub 20241010. doi: 10.1128/jvi.01385-24. PubMed PMID: 39387583; PubMed Central PMCID: PMC11575140.
Henderson R, Anasti K, Manne K, Stalls V, Saunders C, Bililign Y, et al. Engineering immunogens that select for specific mutations in HIV broadly neutralizing antibodies. Nat Commun. 2024;15(1):9503. Epub 20241103. doi: 10.1038/s41467-024-53120-9. PubMed PMID: 39489734; PubMed Central PMCID: PMC11532496.
Vukovich MJ, Shiakolas AR, Lindenberger J, Richardson RA, Bass LE, Barr M, et al. Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families. PLoS Pathog. 2024;20(9):e1012499. Epub 20240918. doi: 10.1371/journal.ppat.1012499. PubMed PMID: 39292703; PubMed Central PMCID: PMC11410209.
Parthasarathy D, Pothula KR, Ratnapriya S, Cervera Benet H, Parsons R, Huang X, et al. Conformational flexibility of HIV-1 envelope glycoproteins modulates transmitted/founder sensitivity to broadly neutralizing antibodies. Nat Commun. 2024;15(1):7334. Epub 20240826. doi: 10.1038/s41467-024-51656-4. PubMed PMID: 39187497; PubMed Central PMCID: PMC11347675.
Parsons RJ, Acharya P. Prying the lid open: Atomic-level insights on sialoglycan-TMPRSS2 coordination in HKU1 entry. Cell. 2024;187(16):4147-9. doi: 10.1016/j.cell.2024.07.007. PubMed PMID: 39121845.
Dutta M, Acharya P. Cryo-electron microscopy in the study of virus entry and infection. Front Mol Biosci. 2024;11:1429180. Epub 20240724. doi: 10.3389/fmolb.2024.1429180. PubMed PMID: 39114367; PubMed Central PMCID: PMC11303226.
Zhang QE, Lindenberger J, Parsons RJ, Thakur B, Parks R, Park CS, et al. SARS-CoV-2 Omicron XBB lineage spike structures, conformations, antigenicity, and receptor recognition. Mol Cell. 2024;84(14):2747-64 e7. doi: 10.1016/j.molcel.2024.06.028. PubMed PMID: 39059371; PubMed Central PMCID: PMC11366207.
Williams WB, Alam SM, Ofek G, Erdmann N, Montefiori DC, Seaman MS, et al. Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Cell. 2024;187(12):2919-34 e20. Epub 20240517. doi: 10.1016/j.cell.2024.04.033. PubMed PMID: 38761800; PubMed Central PMCID: PMC11993910.
Wiehe K, Saunders KO, Stalls V, Cain DW, Venkatayogi S, Martin Beem JS, et al. Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction. Cell Host Microbe. 2024;32(5):693-709 e7. Epub 20240425. doi: 10.1016/j.chom.2024.04.006. PubMed PMID: 38670093.
Parthasarathy D, Pothula KR, Ratnapriya S, Cervera Benet H, Parsons R, Huang X, et al. Conformational flexibility of HIV-1 envelope glycoproteins modulates transmitted/founder sensitivity to broadly neutralizing antibodies. Nat Commun. 2024;15(1):7334. Epub 20240826. doi: 10.1038/s41467-024-51656-4. PubMed PMID: 39187497; PubMed Central PMCID: PMC11347675.
Bennett AL, Edwards R, Kosheleva I, Saunders C, Bililign Y, Williams A, et al. Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv. 2024;10(5):eadj0396. Epub 20240202. doi: 10.1126/sciadv.adj0396. PubMed PMID: 38306419; PubMed Central PMCID: PMC10836732.
May AJ, Acharya P. Structural Studies of Henipavirus Glycoproteins. Viruses. 2024;16(2). Epub 20240127. doi: 10.3390/v16020195. PubMed PMID: 38399971; PubMed Central PMCID: PMC10892422.
Saunders KO, Counts J, Thakur B, Stalls V, Edwards R, Manne K, et al. Vaccine induction of CD4-mimicking HIV-1 broadly neutralizing antibody precursors in macaques. Cell. 2024;187(1):79-94 e24. doi: 10.1016/j.cell.2023.12.002. PubMed PMID: 38181743; PubMed Central PMCID: PMC10860651.
Cell; Volume 187; Issue 16p4147-4149; August 08, 2024; Parsons RJ, Acharya P.
Molecular Cell; Volume 84, Issue 14p2747-2764.e7; July 25, 2024; Zhang et al.
.
2023
Manrique PD, Chakraborty S, Henderson R, Edwards RJ, Mansbach R, Nguyen K, et al. Network analysis uncovers the communication structure of SARS-CoV-2 spike protein identifying sites for immunogen design. iScience. 2023;26(1):105855. Epub 20221226. doi: 10.1016/j.isci.2022.105855. PubMed PMID: 36590900; PubMed Central PMCID: PMC9791713.
Wrapp D, Mu Z, Thakur B, Janowska K, Ajayi O, Barr M, et al. Structure-Based Stabilization of SOSIP Env Enhances Recombinant Ectodomain Durability and Yield. J Virol. 2023;97(1):e0167322. Epub 20230112. doi: 10.1128/jvi.01673-22. PubMed PMID: 36633409; PubMed Central PMCID: PMC9888283.
Pilewski KA, Wall S, Richardson SI, Manamela NP, Clark K, Hermanus T, et al. Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor. Cell Rep. 2023;42(2):112044. Epub 20230127. doi: 10.1016/j.celrep.2023.112044. PubMed PMID: 36708513; PubMed Central PMCID: PMC10372200.
Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, et al. Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage. Nat Commun. 2023;14(1):2782. Epub 20230515. doi: 10.1038/s41467-023-38108-1. PubMed PMID: 37188681; PubMed Central PMCID: PMC10184639.
May AJ, Pothula KR, Janowska K, Acharya P. Structures of Langya Virus Fusion Protein Ectodomain in Pre- and Postfusion Conformation. J Virol. 2023;97(6):e0043323. Epub 20230606. doi: 10.1128/jvi.00433-23. PubMed PMID: 37278642; PubMed Central PMCID: PMC10308951.
Parsons RJ, Acharya P. Evolution of the SARS-CoV-2 Omicron spike. Cell Rep. 2023;42(12):113444. Epub 20231118. doi: 10.1016/j.celrep.2023.113444. PubMed PMID: 37979169; PubMed Central PMCID: PMC10782855.
.
2022
Li D, Sempowski GD, Saunders KO, Acharya P, Haynes BF. SARS-CoV-2 Neutralizing Antibodies for COVID-19 Prevention and Treatment. Annu Rev Med. 2022;73:1-16. Epub 20210824. doi: 10.1146/annurev-med-042420-113838. PubMed PMID: 34428080.
Martinez DR, Schafer A, Gobeil S, Li D, De la Cruz G, Parks R, et al. A broadly cross-reactive antibody neutralizes and protects against sarbecovirus challenge in mice. Sci Transl Med. 2022;14(629):eabj7125. Epub 20220126. doi: 10.1126/scitranslmed.abj7125. PubMed PMID: 34726473; PubMed Central PMCID: PMC8899823.
Murji AA, Raju N, Qin JS, Kaldine H, Janowska K, Fechter EF, et al. Sequence and functional characterization of a public HIV-specific antibody clonotype. iScience. 2022;25(1):103564. Epub 20211203. doi: 10.1016/j.isci.2021.103564. PubMed PMID: 34984325; PubMed Central PMCID: PMC8692997.
Gobeil SM, Henderson R, Stalls V, Janowska K, Huang X, May A, et al. Structural diversity of the SARS-CoV-2 Omicron spike. Mol Cell. 2022;82(11):2050-68 e6. Epub 20220325. doi: 10.1016/j.molcel.2022.03.028. PubMed PMID: 35447081; PubMed Central PMCID: PMC8947964.
Stalls V, Lindenberger J, Gobeil SM, Henderson R, Parks R, Barr M, et al. Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike. Cell Rep. 2022;39(13):111009. Epub 20220608. doi: 10.1016/j.celrep.2022.111009. PubMed PMID: 35732171; PubMed Central PMCID: PMC9174147.
Stalls V, Janowska K, Acharya P. Transient transfection and purification of SARS-CoV-2 spike protein from mammalian cells. STAR Protoc. 2022;3(3):101603. Epub 20220715. doi: 10.1016/j.xpro.2022.101603. PubMed PMID: 35983170; PubMed Central PMCID: PMC9283606.
Saunders KO, Edwards RJ, Tilahun K, Manne K, Lu X, Cain DW, et al. Stabilized HIV-1 envelope immunization induces neutralizing antibodies to the CD4bs and protects macaques against mucosal infection. Sci Transl Med. 2022;14(661):eabo5598. Epub 20220907. doi: 10.1126/scitranslmed.abo5598. PubMed PMID: 36070369; PubMed Central PMCID: PMC10034035.
.
2021
Williams WB, Meyerhoff RR, Edwards RJ, Li H, Manne K, Nicely NI, et al. Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. Cell. 2021;184(11):2955-72 e25. Epub 20210520. doi: 10.1016/j.cell.2021.04.042. PubMed PMID: 34019795; PubMed Central PMCID: PMC8135257.
Weissman D, Alameh MG, de Silva T, Collini P, Hornsby H, Brown R, et al. D614G Spike Mutation Increases SARS CoV-2 Susceptibility to Neutralization. Cell Host Microbe. 2021;29(1):23-31 e4. Epub 20201201. doi: 10.1016/j.chom.2020.11.012. PubMed PMID: 33306985; PubMed Central PMCID: PMC7707640.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, et al. Cold sensitivity of the SARS-CoV-2 spike ectodomain. Nat Struct Mol Biol. 2021;28(2):128-31. Epub 20210105. doi: 10.1038/s41594-020-00547-5. PubMed PMID: 33402708; PubMed Central PMCID: PMC7878407.
Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, et al. D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Cell Rep. 2021;34(2):108630. Epub 20201226. doi: 10.1016/j.celrep.2020.108630. PubMed PMID: 33417835; PubMed Central PMCID: PMC7762703.
Saunders KO, Lee E, Parks R, Martinez DR, Li D, Chen H, et al. Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses. Nature. 2021;594(7864):553-9. Epub 20210510. doi: 10.1038/s41586-021-03594-0. PubMed PMID: 33971664; PubMed Central PMCID: PMC8528238.
Parker R, Partridge T, Wormald C, Kawahara R, Stalls V, Aggelakopoulou M, et al. Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. Cell Rep. 2021;35(8):109179. Epub 20210513. doi: 10.1016/j.celrep.2021.109179. PubMed PMID: 34004174; PubMed Central PMCID: PMC8116342.
Shiakolas AR, Kramer KJ, Wrapp D, Richardson SI, Schafer A, Wall S, et al. Cross-reactive coronavirus antibodies with diverse epitope specificities and Fc effector functions. Cell Rep Med. 2021;2(6):100313. Epub 20210521. doi: 10.1016/j.xcrm.2021.100313. PubMed PMID: 34056628; PubMed Central PMCID: PMC8139315.
Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Stalls V, et al. Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. Science. 2021;373(6555). Epub 20210624. doi: 10.1126/science.abi6226. PubMed PMID: 34168071; PubMed Central PMCID: PMC8611377.
Li D, Edwards RJ, Manne K, Martinez DR, Schafer A, Alam SM, et al. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell. 2021;184(16):4203-19 e32. Epub 20210618. doi: 10.1016/j.cell.2021.06.021. PubMed PMID: 34242577; PubMed Central PMCID: PMC8232969.
Montefiori DC, Acharya P. SnapShot: SARS-CoV-2 antibodies. Cell Host Microbe. 2021;29(7):1162- e1. doi: 10.1016/j.chom.2021.06.005. PubMed PMID: 34265246; PubMed Central PMCID: PMC8279512.
Cottrell CA, Manne K, Kong R, Wang S, Zhou T, Chuang GY, et al. Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies. Cell Rep. 2021;37(5):109922. doi: 10.1016/j.celrep.2021.109922. PubMed PMID: 34731616; PubMed Central PMCID: PMC9058982.
Stalls V, Acharya P. How to train your antibody to fight malaria. Immunity. 2021;54(12):2692-4. doi: 10.1016/j.immuni.2021.11.007. PubMed PMID: 34910937.
Nyanhete TE, Edwards RJ, LaBranche CC, Mansouri K, Eaton A, Dennison SM, et al. Polyclonal Broadly Neutralizing Antibody Activity Characterized by CD4 Binding Site and V3-Glycan Antibodies in a Subset of HIV-1 Virus Controllers. Front Immunol. 2021;12:670561. Epub 20211223. doi: 10.3389/fimmu.2021.670561. PubMed PMID: 35003053; PubMed Central PMCID: PMC8733328.
.
2020
Sempowski GD, Saunders KO, Acharya P, Wiehe KJ, Haynes BF. Pandemic Preparedness: Developing Vaccines and Therapeutic Antibodies For COVID-19. Cell. 2020;181(7):1458-63. Epub 20200527. doi: 10.1016/j.cell.2020.05.041. PubMed PMID: 32492407; PubMed Central PMCID: PMC7250787.
Henderson R, Edwards RJ, Mansouri K, Janowska K, Stalls V, Gobeil SMC, et al. Controlling the SARS-CoV-2 spike glycoprotein conformation. Nat Struct Mol Biol. 2020;27(10):925-33. Epub 20200722. doi: 10.1038/s41594-020-0479-4. PubMed PMID: 32699321; PubMed Central PMCID: PMC8581954.
Henderson R, Lu M, Zhou Y, Mu Z, Parks R, Han Q, et al. Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements. Nat Commun. 2020;11(1):520. Epub 20200124. doi: 10.1038/s41467-019-14196-w. PubMed PMID: 31980614; PubMed Central PMCID: PMC6981184.
.
2019
Saunders KO, Wiehe K, Tian M, Acharya P, Bradley T, Alam SM, et al. Targeted selection of HIV-specific antibody mutations by engineering B cell maturation. Science. 2019;366(6470). doi: 10.1126/science.aay7199. PubMed PMID: 31806786; PubMed Central PMCID: PMC7168753.
Setliff I, Shiakolas AR, Pilewski KA, Murji AA, Mapengo RE, Janowska K, et al. High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity. Cell. 2019;179(7):1636-46 e15. Epub 20191128. doi: 10.1016/j.cell.2019.11.003. PubMed PMID: 31787378; PubMed Central PMCID: PMC7158953.
LaBranche CC, Henderson R, Hsu A, Behrens S, Chen X, Zhou T, et al. Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies. PLoS Pathog. 2019;15(9):e1008026. Epub 20190917. doi: 10.1371/journal.ppat.1008026. PubMed PMID: 31527908; PubMed Central PMCID: PMC6764681.
