Mouse Genes and Mutations:
| Gene/Mutation | Primer ID | PCR product size (kb) | |||
| common/Forward | mutant/Backward | wildtype | mutant | wildtype | |
| E2AER | bjE2Ar3 | YZ-29 | YZ-164 | 1.2 | 0.9 |
| E47bm | YZ-104 | YZ-29 | YZ-164 | 2.0 | 1.7 |
| E2Agal | YZ-177 | YZ-24 | E19 | 0.9 | 1.1 |
| E2Aflox(wt vs. flox) | YZ-104 | YZ-150 | YZ-164 | 2.0 | 1.8 |
| E2Aflox(flox vs. deleted) | YZ-198 | PGKneo for | E2Aflox for | 0.5 (deleted) | 1.2 (flox) |
| E2Adel | E2Aflox for | YZ-198 | E19 | 0.5 | 1.1 |
| E2AGFP | YZ-104 | YZ-29 | YZ-164 | 2.0 | 1.7 |
| E2AHF | YZ-104 | YZ-29 | YZ-164 | 2.0 | 1.7 |
| HEBko | YZ-119 | YZ-29 | YZ-122 | 2.8 | 2.2 |
| *HEBbm | YZ-199 | YZ-122 | 1.8 | 2.0 | |
| HEBflox(wt vs. flox) | JW1 | JW2 | 1.3 | 1.1 | |
| HEBflox(flox vs. deleted) | JW1 | JW2 | 0.5 (deleleted) | 1.3 (flox) | |
| Id2flox (can be combined with Id3flox) | Id2-E2F | Id2-I2R | 0.8 | 0.76 | |
| Id2flox | Id2-Scr1 | Id2-Scr2 | 0.47 | 0.43 | |
| Id3flox | Id3-3 | Id3-4 | 0.65 | 0.515 | |
| Id3ko | YZ-151 | YZ-29 | YZ-170 | 2.5 | 2.1 |
| TCR Vb8.2hCD2 | P1 | P3 | P2 | 1.1 | 1.8 |
| LckCre | Lck-p | Mx3 | 0.8 | ||
| CD4Cre | CD4Crep | Mx3B | 0.45 | ||
| Mx-Cretg | MxCre5 | MxCre3 | 0.5 | ||
| Id2hCD5 | Id2ra | hCD5f | Id2f | 1.2 | 1.5 |
| Id2 GFP # | Id2_wt_F | Id2_wt_R | Id2_GFP_F | 0.65 | 0.371 |
| Id2 ERCre # | Id2_wt_F | Id2_wt_R | Id2_Cre_F | 0.6 | 0.371 |
| Rosa ERCre# | Rosa 1 | Rosa 2 | Rosa 3 | 0.825 | 0.65 |
| Rosa ZsGreen # | ZsG 1 | ZsG 2 | Zsg 3, Zsg 4 | 0.199 | 0.297 |
| PBase # | PBase F | PBase R | 0.85 | ||
| Rosa FLPER (Jax 018906) # | FLPER 1 | FLPER 2 | FLPER 3 | 0.309 | 0.603 |
| FatCre or piggBac Transposon line # | RF1 | RB1 | 0.5 | ||
| Rosa FLP1 # | FLP1A, FLP1B | FLP1C, FLP1D | 0.725 | 0.5 | |
| pre-T alpha ko | pTa-E2S | Neo-A1 | pTa-I2A | 1.39 | 0.85 |
*For HEBbm genotyping, digest with NcoI after PCR to detect mutant vs. wt alleles.
# – Have separate genotyping protocol. See separate section under the PCR Genotyping link below.
