In this protocol paper, we describe details of the GS assay protocol that we originally published in the 2020 Biochemistry paper. The assay is based on size-exclusion chromatography under the basic condition that 1,3-beta-D-glucan is soluble. This approach allows quantitative determination of the amount and the length of glucan produced by yeast GS. In our Biochemistry 2020 paper, we used this assay to study the mechanism of GS catalysis and inhibition.
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