In this paper, we described detailed protocols of expression, purification, and characterization of MoaA and MoaC, as well as the isolation and characterization of 3′,8-cH2GTP. The in situ 13C NMR assay method was also described so that anyone interested can determine the product of MoaA without going through extensive purification in anaerobic glove box. We hope these protocols are informative to whoever interested in Moco biosynthesis or any other related systems.
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Recent Entries
- Matthew and Jib’s work is published online in Nature Chemical Biology
- Our Comprehensive Natural Products reviews are published online
- Haoran and Edward’s work on MoaA is published online in JACS
- Abhi successfully defended his PhD dissertation
- Dr. Ogasawara visited Duke to give a seminar
- Abhi and Anna’s paper on glucan synthase was published in Biochemistry.
- Hai and Matthew won poster award!
- Abhi won a poster prize in the 2019 Enzymes Coenzymes and Pathway GRC
- Hai’s paper on mitomycin biosynthesis was published
- Ken was selected as the 2019 ACS Pfizer Award in Enzyme Chemistry
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