Short clip put together by the media group at Duke University about the use of anchor cell invasion in understanding metastasis in cancer.
One hour time-lapse of invasive structures (“invadosomes”, PIP2 labeling, cyan) and basement membrane (laminin::GFP, magenta) shows the anchor cell breaching the basement membrane in the left panel. Upper right panel is the same anchor cell with a spectral representation of fluorescent intensity highlighting the invadosomes in green and red. On the lower right panel is the basement membrane shown ventrally. 4D movies generated with Imaris software (movie by Elliott Hagedorn).
One hour time-lapse of invasive structures (“invadosomes”, PIP2 labeling, cyan). Basement membrane (magenta), anchor cell (cyan). We use isosurface rendering (yellow) with Imaris software to quantitatively examine invadosome dynamics in wild-type and mutant animals (movie by Elliott Hagedorn).
- Basement membrane gap created by the anchor cell at L3 stage
- Basement membrane gap expanding beyond the anchor cell by the late L3
- Basement membrane gap expands well beyond the anchor cell by the early L4 stage
Anchor cell invasion creates a breach in the basement membrane at the mid L3 larval stage and initiates contact with the underlying vulval cells. Shortly after the anchor cell breaches the basement membrane, the anchor cell expands the breach. After the gap in the basement membrane widens to the edge of the anchor cell, the gap continues to expand over the vulval cells (by sliding) until it reaches the vulD cells, where it is stabilized by integrin adhesion.
Why study anchor cell invasion.