Inhalation and Respiratory Therapy

pulminaryDuke Rodent Inhalation and Physiology Core Resource Facility

The Pre-Clinical Translational Research Unit (PTRU) has access to a new state of the art rodent inhalation and physiology core facility, the unit co-directors are Drs. Loretta G. Que and Robert M. Tighe. Investigators can submit requests for the Rodent Inhalation Core services at CoreResearch@Duke. The facility was created by the School of Medicine to provide Duke Investigators and its partners with expertise in rodent inhalation, injury and therapeutic models. Pulmonary specialists and skilled technical staff with greater than 10 years of experience provide all core services. This core offers comprehensive services from study design and development, to performance of exposures and phenotyping, to data analysis and interpretation.

Services Provided

  • Inhalation models of allergic and non-allergic environmental exposures
  • Assessment of airway physiology and lung compliance using the FlexiVentâ system for rodents (mice and rats)
  • Rodent tissue harvest including blood, bronchoalveolar lavage fluid, and lung tissue
  • Preparation of lung tissues for histopathology
  • Performance of bronchoalveolar lavage total cell counts and differentials
  • Consultative services for pulmonary data analysis and interpretation
  • New Pulmonary Exposure Model Development including delivery of aerosolized therapeutics

Infectious Exposure Models

  • Lipopolysaccharide (LPS)
  • Influenza-Induced Lung Injury

Allergic Exposure Models (acute and chronic):

  • OVA (ovalbumin)
  • HDM (house dust mite)
  • HDM/(LPS
  • Cockroach Antigen
  • Fungal Antigen Proteinase (FAP)
  • Pollen Extract
  • High fat fed diet induced obesity (HFD)

Non-Allergic Exposure/Injury Models (acute and chronic):

  • Ozone
  • Chlorine gas
  • Phosgene gas
  • E-cigarettes (vaping)
  • Woodsmoke
  • Tobacco Smoke
  • Ventilator Induced Lung Injury