The Kinesin-5 (formerly BimC) family tree was obtained using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-5 proteins from humans and several model organisms. Kinesin-5 proteins from other organisms are also shown in the table and sequence alignment below.
Species/protein | Molecular mass (kDa) | Motor polarity & velocity | Subcellular localization | Comments |
---|---|---|---|---|
S. pombe Cut7 | 121 | ND | Spindle, poles | Required for SPB separation and spindle formation |
A. nidulans bimC | 132 | ND | Functions in pole separation | Essential for SPB separation in mitosis, mutants arrest with monoploar spindles |
S. cerevisiae Kip1 | 126 | ND | Spindle, poles | Redundant with Cin8 |
H. sapiens KIF8 | ||||
S. cerevisiae Cin8 | 118 | ND | Spindle | Required for separation of spindle poles, formation & maintenance of bipolar spindle |
P. Falciparum MAL3P6.13 | 192 | |||
C. elegans F23B12.8 | 108 | ND | ND | Fused with CeF08H9.1 |
H. sapiens KSP (HsEg5) | 121 | Plus, 2 µm/min | ND | Essential for centrosome separation in mitosis, mutants arrest with monopolar spindle |
D. melanogaster Klp61F (KRP130) | 121 (130) | Plus, 2 µm/min | ND | Essential for centrosome separation in mitosis, mutants arrest with monopolar spindle, homotetramer |
A. thaliana F16L2.60 | 119 | |||
A. thaliana KRP125a | 116 | |||
A. thaliana KRP125b | 119 | |||
A. thaliana KRP125c | 120 | |||
B. mori Krp | ND | ND | ND | |
N. tabacum KRP125 | 114 | ND | ND | |
X. laevis Eg5 | 119 | Plus, 2 µm/min | Spindle MTs | Required for spindle formation in vitro at mitosis |
X. laevis Eg52 | 120 | ND | Oocytes, eggs |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' SpoCUT7 NETNINVVVRVRGRTDQEVRDNSSLAVSTSGAMGAELAIQS-----DPSSMLVTKTYAFDKVFGPEADQLMLFENSVAPMLEQVLNGYNCTIFAYGQTGTGKTYTMSGD-------LSDSDGILSEGAGLIPRALYQLFSSLDNSNQE-YAVKCSYYELYNEEIRDLLVSEE-------------------------------------------------------------------------------------------------------LRKPARVFEDTSRRGNVVITGIEESYIKNAGDGLRLLREGSHRRQVAATKCNDLSSRSHSIFTITLHRKVSSGMTDETNSLTINNNSDDLLRASKLHMVDLAGSENIGRSG----AENKRARETGMINQSLLTLGRVINALVEKAHHIPYRESKLTRLLQDSLGGKTKTSMIVTVSSTNTNLEETISTLEYAARAKSIRNKPQNN SpoCUT7 AnBIMC EDTSIHVVVRCRGRNEREVKENSGVVLQTEGVKGKTVELSM------GPNAVSNKTYTFDKVFSAAADQITVYEDVVLPIVTEMLAGYNCTIFAYGQTGTGKTYTMSGD-------MTDTLGILSDNAGIIPRVLYSLFAKLADTEST---VKCSFIELYNEELRDLLSAEE------------------------------------------------------------------------------------------------------NPKLKIYDNEQKKGHMSTLVQGMEETYIDSATAGIKLLQQGSHKRQVAATKCNDLSSRSHTVFTITVNIKR------------TTESGEEYVCPGKLNLVDLAGSENIGRSG----AENKRATEAGLINKSLLTLGRVINALVDKSQHIPYRESKLTRLLQDSLGGRTKTCIIATMSPARSNLEETISTLDYAFRAKNIRNKPQIN AnBIMC ScKIP1 SDSNIHVYVRCRSRNKREIEEKSSVVISTLGPQGKEIILSN---GSHQSYSSSKKTYQFDQVFGAESDQETVFNATAKNYIKEMLHGYNCTIFAYGQTGTGKTYTMSGDINILGDVQSTDNLLLGEHAGIIPRVLVDLFKELSSLNKE-YSVKISFLELYNENLKDLLSDSE-----------------------------------------------------------------------------------------------DDDPAVNDPKRQIRIFDNNNNNSSIMVKGMQEIFINSAHEGLNLLMQGSLKRKVAATKCNDLSSRSHTVFTITTNIVE--------QDSKDHGQNKNFVKIGKLNLVDLAGSENINRSG----AENKRAQEAGLINKSLLTLGRVINALVDHSNHIPYRESKLTRLLQDSLGGMTKTCIIATISPAKISMEETASTLEYATRAKSIKNTPQVN ScKIP1 ScCIN8 EELNITVAVRCRGRNEREISMKSSVVVNVPDITGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQDLIFDEVAGPLFQDFIKGYNCTVLVYGMTSTGKTYTMTGD-------EKLYNGELSDAAGIIPRVLLKLFDTLELQQND-YVVKCSFIELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFASSTANNTTSNSASSSRSNSRNSSPRSLNDLTPKAALLRKRLRTKSLPNTIKQQYQQQQAVNSRNNSSSNSGSTTNNASSNTNTNNGQRSSMAPNDQTNGIYIQNLQEFHITNAMEGLNLLQKGLKHRQVASTKMNDFSSRSHTIFTITLYKKH----------------QDELFRISKMNLVDLAGSENINRSG----ALNQRAKEAGSINQSLLTLGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTALIATISPAKVTSEETCSTLEYASKAKNIKNKPQLG ScCIN8 CeF23B12 PTSNLRVAVRIRPMNGTERSEKCTNVVKVDKGK-------------QAIELKGKSFGPFFRTYDPDTTQEEIYSDLVSSQIKKVIAGFNCTVFAYGQTGTGKTFTMEGG------RTDAKSSTDDPTTGIIPRAVEDIFEQLERCGCEEYSLRVSYIELYNEELFDLLASTD------------------------------------------------------------------------------------------------------NEDRERLRIFDDPNKKGVIVSGVEEVPVRNRSDVFKLLQLGAEKRRTAATLMNMHSSRSHSLFMVNVVIRE------------NTTTGEELVKQGKLNLVDLAGSENIGRSG----AQGNRAKEAGSINQSLLTLGRVIRLLTTNGQHIPYRESKLTRLLQDSLGGSTITSLIATLSPSSSNFEESQSTLEYAMRAANIKNKPVCN CeF23B12 HsKSP KGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTG-----GLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTE-FSVKVSLLEIYNEELFDLLNPSS------------------------------------------------------------------------------------------------------DVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKE------------TTIDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVN HsKSP DmKLP61F SNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTL--------DSKLTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGN----ETAELKSSWEDDSDIGIIPRALSHLFDELRMMEVE-YTMRISYLELYNEELCDLLSTDD--------------------------------------------------------------------------------------------------------TTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRE------------NGIEGEDMLKIGKLNLVDLAGSENVSKAG---NEKGIRVRETVNINQSLLTLGRVITALVDRAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVN DmKLP61F BmKRP KNQNIQVFVRLRPLNQRERDLKSLGVVEVHNNKEVVVRISQ--------QNSITKKFTFDRAFAPYANQVEVYQEVVSPLIEEVLAGYNCTVFAYGQTGTGKTHTMVGE-----NTGDETTWQKDPLAGIIPRALSQLFDELRISNTE-YTVRVSYLELYNEELFDLLATSE-------------------------------------------------------------------------------------------------------DNSKLRIYEDVTRKGSNIVNGLEEITVYNKKEVFRIMAQGQERKKVASTLMNAQSSRSHTVFTIVVHMKE-----------NSLPEGEELVKIGKLNLVDLAGSENISKAGSDNPAKRERARECVNINQSLLTLGRVITALVERHPHVPYRESKLTRILQESLGGRTKTSIIATISPGHKDLEETMSTLEYLTEQRTFRTSLRST BmKRP NtKRP125 KGVNVQVLLRCRPFSNDELRNNAPQVVTCNDYQREVAVSQN------IAGKHIDRIFTFDKVFGPSAQQRDLYDQAIVPIVNEVLEGFNCTIFAYGQTGTGKTYTMEGE---CKRSKSGPNGELPQEAGVIPRAVKQVFDTLESQNAE-YSVKVTFLELYNEEITDLLAPED-------------------------------------------------------------------------------------------------LKVALEDRQKKQLPLMEDGKGGVLVRGLEEEIVTSANEIFTLLERGSAKRRTAETLLNKQSSRSHSLFSITIHIKE------------ATPEGEELIKCGKLNLVDLAGSENISRSG----AREGRAREAGEINKSLLTLGRVINALVEHLGHIPYRDSKLTRLLRDSLGGRTKTCIIATVSPAVHCLEETLSTLDYAHRAKNIKNKPEVN NtKRP125 XlEg5 KGKNIQVVVRCRPFNQLERKASSHSVLECDSQRKEVYVRTG-----EVNDKLGKKTYTFDMVFGPAAKQIEVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSSDEEFTWEQDPLAGIIPRTLHQIFEKLSENGTE-FSVKVSLLEIYNEELFDLLSPSP------------------------------------------------------------------------------------------------------DVGERLQMFDDPRNKRGVIIKGLEEISVHNKDEVYHILERGAAKRKTASTLMNAYSSRSHSVFSVTIHMKE------------TTVDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERAPHIPYRESKLTRILQDSLGGRTKTSIIATVSPASINLEETVSTLDYASRAKSIMNKPEVN XlEg5 XlEg52 KGKNIQVVVRCRPFNQLERKASSHSVLECESQRKEVCVRTG-----EVNDKLGKKTYTFDMVFGPAAKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGE----RSSDEEFTWEQDPLAGIIPRTLHQIFEKLSEIGTE-FSVKVSLLEIYNEELFDLLSPSP------------------------------------------------------------------------------------------------------DVGERLQMFDDPRNKRGVIIKGLEEISVHNKDEVYQILERGAAKRKTASTLMNAYSSRSHSVFSVTIHMKE------------TTIDGEELVKIGKLNLVDLAGSENIGRSG----AVDKRAREAGNINQSLLTLGRVITALVERAPHIPYRESKLTRILQDSLGGRTKTSIIATVSPASINLEETMSTLDYASRAKNIMNKPEVN XlEg52
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:23 GMT