The Kinesin-6 (formerly MKLP1) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-6 proteins from humans and several model organisms. Kinesin-6 proteins from other organisms are also shown in the table and sequence alignment below.
|
Species/protein |
Molecular mass (kDa) |
Motor polarity & velocity |
Subcellular localization |
Comments |
|
|---|---|---|---|---|---|
| D. melanogaster PAV-KLP | 101 | ND | |||
| H.sapiens MKLP1 | 110 | Plus, 4 µm/min | Spindle, poles and midbody | Separates poles at anaphase B? | |
| C. elegans Zen4 (M03D4.1) | ND | ND | ND | ||
| C. griseus CHO1 | 109 | ND | Spindle, midbody | Separates poles at anaphase B? | |
| D. rerio ZfMKLP1 | ND | ND | ND |
1 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' DmPAV ARDPVNVFCRVRPLQSDADLTSLRVKNSTTIALNPQDQLLQHHKPHNGAQREVQYIFKHVFQPDATQQDVYAAVAQPLVENLLKGRNSLLFTYGVTGSGKTYTMTGNL--RHRGIMPRCLDVLFRTISDYQAKKFVFKPDKLNGFEILSEEDALLERQHEMNQRFAGSGRFAFRHKDSDPEIASQASVEPIPLLGLDEDNMYSVFVTYIEIYNNSVYDLLEDSGIQ----KTLQSKIIREDANRHMFVHGVTEVEVKTVEDALEVFQMGQKRKRMGHTVLNAESSRSHSVFNIRLVQAPTD--SQGENVVQDRQNITVSQLSLVDLAGSERSSRTKNTGVRLREAGNINNSLMTLRTCLEYLRENQLAASNGLAPKKVPYRDSKITHMFKNYFDGEGQVSMIVCINPRIEDYDENMQVMKFAEMTQEVQIARATP DmPAV HsMKLP1 LKTQLG-YCRVR-LGFPDQECCIEVINNTTVQLHTPEGYRL---NRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMP--NPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPINPNLHNLNCFVKIKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLD--ADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQ----MYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVD HsMKLP1 CeM03D4.1 EETDSIEVVCRLCPYTGSTPSLIAIDEGSIQTVLPPAQFRR----ENAPQVEKVFRFGRVFSENDGQATVFERTSVDLILNLLKGQNSLLFTYGVTGSGKTYTMTGKPTETGTGLLPRTLDVIFNSINNRVEK-CIFYPSALNTFEIRATLDAHLKRHQMAADR------------LSTSREITDRYCEAIKLSGYNDDMVCSVFVTYVEIYNNYCYDLLEDAR-----NGVLTKREIRHDRQQQMYVDGAKDVEVSSSEEALEVFCLGEERRRVSSTLLNKDSSRSHSVFTIKLVMAPRAYETKSVYPTMDSSQIIVSQLCLVDLAGSERAKRTQNVGERLAEANSINQSLMTLRQCIEVLRRNQ--KSSSQNLEQVPYRQSKLTHLFKNYLEGNGKIRMVICVNPKPDDYDENMSALAFAEESQTIEVKKQVE CeM03D4.1 CgCHO1 LKDPVGVYCRVRPLSCPDQECCVEVVSNTTVQLHTPEGYRL---NRNGDYKETQYSFKQVFGTHTTQKELFEVVASPLVDDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GSGGLLPRCLNMIFNSIGSFQAKRYVFKSNDRNSMEIQCEVDALLERQKREAVS--IPKTPSSKRQADPEFSDMINVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVQFDPIKPKLPQSKILREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFSIKLVQAPLD--ADGDNVLQEKEQITISQLSLVDLAGSERTNRTKAEGNRLREAGNINQSLMTLRTCMEVLRENQ----TYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEESLQVMRFAEVTQEVEVARPVD CgCHO1 ZfMKLP1 QKDPVGVYCRVRPLGAEDEECCIEVISNTTIQLHAPDGLKA---NRNGEFKETQYSFKKVFGIKTTQRELFEDVAKPLAQDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GQGGLLPRSLDMIFNSIGPYQAKRYVFKPDDKNGMEVQNQVDALLDRQKRDSQT--SVPKTPNTRRVDPEFADMISPEEACKAEGVDEDSSYSVFVSYIEIYNNYIYDLLEETPFDPIKPKPPQSKILREDQNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTQLNRESSRSHSVFIVKLAQAPLD--ADGDNVLQDKNQVNVSQLCLVDLAGSERTSRTRAEGNRLREAGNINQSLMTLRTCIEVLRENQ----MCGTNKMVPYRDSKVTHLFKNYFDGEGKVRMVVCVNPKADDYEETLLVMRFAEMTQEVEVARPVD ZfMKLP1
If you use diagrams, trees, or sequence alignments from the Kinesin site, we ask that you cite either the home page and authors, or the appropriate source publication in your work.
Copyright 1996-2005. All rights reserved.
Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:24 GMT
