Axoneme-MTs moving on Kinesin-1 bound to a coverslip

Axoneme-MT complexes were assembled with the axoneme fragment (denser region) at the minus end of the complex and the microtubule at the plus ends. The axoneme-MT complexes attach to purified bovine brain Kinesin-1 bound to a glass coverslip and move with the axoneme (minus) ends leading. Because Kinesin-1 is immobilized on the glass surface and the axoneme-MTs are moving on the immobilized motor, movement of the complexes with the axoneme leading shows that Kinesin-1 moves on microtubules towards the plus ends. The sequence shows two axoneme-MTs, both moving with the axoneme leading, and a single microtubule without an axoneme. The sequence has been speeded up by 12.5X real time. For further information regarding Kinesin-1 motility, go to the motility page. Contributed by Sharyn Endow and Hebok Song.

Return to the Kinesin Movie Page.

Return to the Kinesin Home Page.

If you use images or other material from the reviews at the Kinesin site, we ask that you cite either the home page and author, or the appropriate source publication in your work.

Copyright 1996-2003. All rights reserved.

Created 5 October 1996 4:00 GMT
Modified 29 October 2004 20:55 GMT