The Duke Center for Genomic and Computational Biology and the GCB have established a shared resource for the application of functional genomic technologies to support the research programs of investigators at Duke and beyond. Through investments in RNAi and complementary technologies, we have assembled state-of-the-art reagents and equipment, providing researchers access to genome-wide RNAi reagents and the infrastructure necessary to conduct large-scale functional studies in mammalian cells.
- RNAi expertise: The facility serves as the focal point of RNAi technology development and expertise for the Duke University and Medical Center research community, and will work to advance and apply gene silencing technologies to a broad range of research programs.
- RNAi reagents: The availability of RNAi libraries targeting entire genomes or subsets provides the opportunity to conduct loss-of-function genetic screens and selections in mammalian cells. The Functional Genomics Shared Resource has several human genome RNAi collections: The Qiagen Human whole-genome siRNA library v1.0, the TRC1 lentiviral shRNA library and an MSCV-based retroviral shRNA library. The siRNA library may be screened in arrayed format, subsets of the TRC1 library may be screened in arrayed or pooled format and the MSCV retroviral library is available only in pooled format.
- Genome-wide RNAi studies: The Functional Genomics Shared Resource has developed the infrastructure necessary to conduct high-throughput RNAi based screening on a genome-wide scale. In addition, we have the ability to perform high-content cell-based imaging and analysis. The facility will work with researchers to develop, evaluate, and implement cell-based screening and/or selection studies.
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