Duke Viral Vector Core

We offer lentiviral vector particles in the following formats

• Small Non-Concentrated Lentivirus Preps (volume-10mL, at the concentration 2.5 x10⁷ vg/mL)

Great for the transduction into human cell lines, such as HEK293, HELA, HT-29 and others. Vectors will be supplied in the aliquots of 1 mL. Vectors will be tittered by ELISA P²⁴ assay and/or real-time PCR.

Production time: 7 days

• Large Non-Concentrated Lentivirus Preps (volume-100mL, at the concentration ≥ 2.5 x10⁷ vg/mL). Vectors will be supplied in the aliquots of 10 mL. Vectors will be tittered by ELISA P²⁴ assay and/or real-time PCR.

Production time: 7 days.

• Concentrated Lentivirus In-Vitro Grade Preps (volume-100uL, at the concentration ≥ 2.5 x10⁹ vg/mL).
• Vectors of this grade will be suitable for in vitro studies and can be used to transduce human non-permissive cells (for example, some primary cells) or cells of not human origin (NIH3T3 fibroblasts, mouse MEFs, mouse C2C12 cells, and others).

Note: this grade is not suitable for the transduction into delicate cells, such as primary neurons or for in-vivo applications. Vectors will be supplied in the aliquots of 20 uL. Vectors will be tittered by ELISA P²⁴ assay and/or real-time PCR. Production time: 2 weeks.

• Small Concentrated Lentivirus In-Vivo Grade Preps (volume-25uL, at the concentration ≥ 2.5 x10⁹ vg/mL).

• Vectors of this grade will be suitable for the transduction is suitable for in vitro studies and can be used to transduce human non-permissive cells (for example, some primary cells) or cells of not human origin (NIH3T3 fibroblasts, mouse MEFs, mouse C2C12 cells, and others).  This grade is suitable for the transduction into delicate cells, such as neurons and for in vivo studies. This vector grade greatly fits small in-vivo pilot experiments. Vectors will be supplied in the aliquots of 5 uL. Vectors will be tittered by ELISA P²⁴ assay and/or real-time PCR.

Note: The cost includes testing for appearance of Replication Competent Retrovirus (RCR) – following FDA recommendations in “Guidance for Industry—Supplemental Guidance on Testing for Replication Competent Retrovirus in Retroviral Vector Based Gene Therapy Products and During Follow-up of Patients in Clinical Trials Using Retroviral Vectors” (Gunter et al. 1993) and the Vaccines and Related Biological Products Advisory Committee (October 25, 1993). Production time: 2 weeks.

• Medium (Standard) Concentrated Lentivirus In-Vivo Grade Preps (volume-150uL, at the concentration ≥ 2.5 x10⁹ vg/mL).

Vectors of this grade will be suitable for in-vivo studies and also can be used to transduce human non-permissive cells (for example, some primary cells) or cells of not human origin (NIH3T3 fibroblasts, mouse MEFs, mouse C2C12 cells, and others). Vectors will be supplied in the aliquots of 15 uL. Vectors will be tittered by ELISA P24 assay and real-time PCR.

The cost includes testing for appearance of Replication Competent Retrovirus (RCR)

Production time: 2 weeks.

• Large Concentrated Lentivirus In-Vivo Grade Preps (volume-450uL, at the concentration ≥ 5 x10⁹ vg/mL).

Vectors of this grade will be suitable for large-scale in vivo studies. Vectors will be supplied in the aliquots of 25 uL. Vectors will be tittered by ELISA P24 assay and real-time PCR.

The cost includes testing for appearance of Replication Competent Retrovirus (RCR)

Production time: 3 weeks.

* To generate customized vector at this grade, we will need at least 1.65 mg of plasmid at concentration ≥500 ng/uL prepared using commercially-available kits or using cesium gradient protocol.

GRADE TABLE