Cell and Gene Modification Core

Director:
Chuan-Yuan Li, PhD
Professor of Dermatology
Vice-Chair for Research,
Department of Dermatology
Chuan.li@duke.edu

Co-Director:
Charles A. Gersbach, PhD
Assistant Professor of Biomedical Engineering
Department of Biomedical Engineering
charles.gersbach@duke.edu

Manager:
Fang Li, PhD
Assistant Professor of Dermatology
Department of Dermatology
fang.li@duke.edu

The Cell and Gene Modification Core provides state-of-the-art gene modification services to Duke Pinnell Center members to facilitate their skin-related research projects. A variety of cell and gene modification services are provided. Examples of the services include:

  • Provide consultation on the most suitable technology for gene modification.
  • Provide plasmid- or lentivirus- based reporter genes for members to label their cells for a variety of purposes.
  • Provide consultation on the use of CRISPR technology to modify/knockout target genes.
  • Provide plasmid construction service for genome editing (CRISPR-based) in immortalized cells.
  • In collaboration with the Duke Viral Vector Core, a School of Medicine supported service, to generate recombinant lentiviral vectors encoding CRISPR gene targeting components.

Pricing:
Consultation:
Free

Vector construction:
Starting at $500 for basic insertions. Price includes designing, ordering, constructing, and sequencing verification of vectors. Additional charge may apply if large quantity plasmids (midi-prep or above) are needed for lentiviral preparation.

Lentiviral vector production:
To be done in collaboration with Duke Viral Vector Core.