Select peer-reviewed publications (from 312 total)


312. Cullen, Bryan R. (2017) RNA interference in mammals: The virus strikes back. Immunity, 46: 970-972.

311. Kennedy, Edward, Courtney, David G., Tsai, Kevin and Cullen, Bryan R. (2017) Viral epitranscriptomics. Journal of Virology, 91: e02263-16.

310.   Bogerd, Hal P., Kennedy, Edward M., Whisnant, Adam W. and Cullen, Bryan R. (2017) Induced packaging of cellular microRNAs into HIV-1 virions can inhibit infectivity. mBio, 8: e02125-16.

309.   Kennedy, Edward M., Kornepati, Anand V., Bogerd, Hal P. and Cullen, Bryan R. (2017) Partial reconstitution of the RNAi response in human cells using Drosophila gene products. RNA, 23: 153-160.

308.   Kennedy, Edward M. and Cullen, Bryan R. (2017) Gene editing: A new tool for viral disease. Annual Review of Medicine, 68: 401-411.



307.   Kennedy, Edward M., Bogerd, Hal P., Kornepati, Anand V.R., Kang, Dong, Ghoshal, Delta, Marshall, Joy B., Poling, Brigid C., Tsai, Kevin, Gokhale, Nandan, Horner, Stacy M., Cullen, Bryan R. (2016) Post transcriptional m6a editing of HIV mRNA enhances viral gene expression.   Cell Host and Microbe, 19(5):675-85.



306. Bogerd, Hal P., Kornepati, Anand V. R., Marshall, Joy B., Kennedy, Edward M. and Cullen, Bryan R. (2015) Specific induction of endogenous viral restriction factors using CRISPR Cas-derived transcriptional activators. Proc Natl Acad Sci U S A, 112: E7249-56.

305. Kennedy, Edward M, Whisnant, Adam W., Kornepati, Anand V.R., Marshall, Joy B., Bogerd, Hal P. and Cullen, Bryan R. (2015). Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer. Proc Natl Acad Sci U S A, 112: E6945-54.

304. Friedland, Ari E, Baral, Reshica, Singhal, Pankhuri, Loveluck, Katherine, Shen Sshen, Sanchez Minerva, Marco, Eugenio, Gotta, Gregory M. Maeder, Morgan L., Kennedy, Edward M., Kornepati, Anand V. R., Sousa, Alexander, Collins, McKensie A., Jayaram, Hari, Cullen, Bryan R. and Bumcrot, David (2015). Characterization of Staphylococcus aureus Cas9 – a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications. Genome Biology, 16:257.

303. Kennedy, Edward M., Kornepati, Anand V. R. and Cullen, Bryan R. (2015) Targeting hepatitis B virus cccDNA using CRISPR Cas9. Antiviral Research, 123: 188-192.

302. Gregorovic, Goran, Boulden, Elizabeth, Bosshard, Rachel, Karstegl, Claudio Elgueta, Skalsky, Rebecca, Cullen, Bryan, Gujer, Cornelia, Rämer, Patrick, Münz, Christian and Farrell, Paul (2015) Epstein-Barr viruses deficient in EBER RNAs give higher LMP2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infection in humanized mice. Journal of Virology, 89: 11711-11714.

301. Mefferd, Adam L., Kornepati, Anand V. R., Bogerd, Hal P., Kennedy, Edward M. and Cullen, Bryan R. (2015) Expression of CRISPR Cas single guide RNAs using small tRNA promoters, RNA, 21: 1683-1689.

300. Kennedy, Edward M., Kornepati, Anand V. R., Mefferd, Adam L., Marshall, Joy B., Tsai, Kevin, Bogerd, Hal P. and Cullen, Bryan R. (2015) Optimization of a multiplex CRISPR Cas for use as an antiviral therapeutic, Methods, 91: 82-86.

299.  Skalsky, Rebecca L. and Cullen, Bryan R. (2015) EBV noncoding RNAs. Current Topics in Microbiology and Immunology. 391: 181-217.

298.  Kang, Dong, Skalsky, Rebecca L. and Cullen, Bryan R. (2015) EBV BART microRNAs target multiple pro-apoptotic cellular genes to promote epithelial cell survival. PLoS Pathogens, 11, e1004979.

297.  Kennedy, Edward M. and Cullen, Bryan R. (2015) Bacterial CRISPR/Cas DNA endonucleases: A revolutionary technology that could dramatically impact viral research and treatment. Virology, 479-480: 213-220.

296.  Cullen, Bryan R. (2015) The virology:RNA biology connection. RNA, 21: 592-594.

295.  Kennedy, Edward M., Bassit, Leda C., Mueller, Henrik, Kornepati, Anand V. R., Bogerd, Hal P., Nie, Ting, Chatterjee, Payel, Javanbakht, Hassan, Schinazi, Raymond F. and Cullen, Bryan R. (2015) Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease. Virology, 476, 196-205.


294. Cullen, Bryan R. (2014) HIV-1 packing to leave. Cell, 159: 975-976.

293. Cullen, Bryan R. (2014) Viruses and RNA interference: Issues and controversies. Journal of Virology, 88, 12934-12936.

292. Kennedy, Edward M., Kornepati, Anand V. R., Goldstein, Michael, Bogerd, Hal P., Poling, Brigid C., Whisnant, Adam W., Kastan, Michael B. and Cullen, Bryan R. (2014) Inactivation of the human papillomavirus E6 or E7 gene in cervical carcinoma cells using a bacterial CRISPR/Cas RNA-guided endonuclease. Journal of Virology, 88, 11965-11972.

291. Furuse, Yuki, Finethy, Ryan, Saka, Hector A., Xet-Mull, Ana M., Sisk, Dana M., Smith, Kristen L. Jurcic, Lee, Sunhee, Coers, Jörn, Valdivia, Raphael H., Tobin, David M. and Cullen, Bryan R. (2014) Search for microRNAs expressed by intracellular bacterial pathogens in infected mammalian cells. PLoS One, 9:e106434.

290. Hal P. Bogerd, Rebecca L. Skalsky, Edward M. Kennedy, Yuki Furuse, Adam W. Whisnant, Omar Flores, Kimberly L. W. Schultz, Nicole Putnam, Nicholas J. Barrows, Barbara Sherry, Frank Scholle, Mariano A. Garcia-Blanco, Diane E. Griffin, Bryan R. Cullen. (2014) Replication of Many Human Viruses Is Refractory to Inhibition by Endogenous Cellular MicroRNAs. Journal of Virology, 88, 8075-8076.

289. Skalsky, Rebecca L., Olson, Ken E., Blair, Carol D., Garcia-Blanco, Mariano A. and Cullen, Bryan R. (2014) A “microRNA-like” small RNA expressed by Dengue virus? Proceedings of the National Academy of Sciences, USA, 111 (23) E2360.

288. Interview with Bryan R. Cullen (2014) Future Virology, 9, 345-350.

287. Bogerd, Hal P., Whisnant, Adam W., Kennedy, Edward M., Flores Omar and Cullen, Bryan R. (2014) Derivation and characterization of Dicer- and microRNA-deficient human cells. RNA, 6 (20), 1–15.

286. Pan, Dongli, Flores, Omar, Umbach, Jennifer L., Pesola, Jean M., Bentley, Peris, Rosato, Pamela C., Leib, David A., Cullen, Bryan R. and Coen, Donald M. (2014) A neuron-specific host microRNA targets herpes simplex virus-1 ICP0 expression and promotes latency. Cell Host & Microbe, 15, 446-456.

285. Flores, Omar, Kennedy, Edward M., Skalsky, Rebecca L. and Cullen, Bryan R. (2014) Differential RISC association of endogenous human microRNAs predicts their inhibitory potential. Nucleic Acids Research, 42, 4629-4639.

284. Whisnant, Adam W., Kehl, Timo, Bao, Qiuying, Materniak, Magdalena, Kuzmak, Jacek, Löchelt, Martin and Cullen, Bryan R. (2014) Identification of novel, highly expressed retroviral microRNAs in cells infected by bovine foamy virus. Journal of Virology, 88, 4679-4686.

283. Furuse, Yuki, Dastjerdi, Akbar, Seilern-Moy, Katharina, Steinbach, Falko and Cullen, Bryan R. (2014) Analysis of viral microRNA expression by elephant endotheliotropic herpesvirus 1. Virology, 454-455, 102-108.

282. Parnas, O., Corcoran, David L. and Cullen, Bryan R. (2014) Analysis of the mRNA targetome of microRNAs expressed by Marek’s disease virus. mBio, 5, 01060-13.

281. Skalsky, Rebecca L., Kang, Dong, Linnstaedt, Sarah D. and Cullen, Bryan R. (2014) Evolutionary conservation of primate lymphocrytovirus microRNA targets. Journal of Virology, 88, 1617-1635.


280. Cullen, Bryan R. (2013) How do viruses avoid inhibition by endogenous cellular microRNAs? PLoS Pathogens, e1003694.

279. Furuse, Yuki, Ornelles, David A. and Cullen, Bryan R. (2013) Persistently adenovirus-infected lymphoid cells express microRNAs derived from the viral VAI and especially VAII RNA. Virology, 447, 140-145.

278. Cullen, Bryan R., Cherry, Sara and tenOever, Benjamin R. (2013) Is RNA interference a physiologically relevant innate antiviral immune response in mammals? Cell Host & Microbe, 14, 374-378.

277. Jin, Hyun Yong, Oda, Hiroyo, Lai, Maoyi, Skalsky, Rebecca L., Bethel, Kelly, Shepherd, Jovan, Kang, Seung Goo, Liu, Wen-Hsien, Sabouri-Ghomi, Mohsen, Cullen, Bryan R., Rajewsky, Klaus and Xiao, Changchun. (2013) MicroRNA-17~92 plays a causative role in lymphomagenesis by coordinating multiple oncogenic pathways. EMBO Journal , 32: 2377-2391.

276. Majoros, William H., Lekprasert, Parwee, Mukherjee, Neelanjan, Skalsky, Rebecca L., Corcoran, David L., Cullen, Bryan R. and Ohler, Uwe. (2013) MicroRNA target site identification by integrating sequence and binding information. Nature Methods, 10: 630-633.

275. Flores, Omar, Nakayama, Sanae, Whisnant, Adam W., Javanbakht, Hassan, Cullen, Bryan R. and Bloom, David C. (2013) Mutational inactivation of HSV-1 microRNAs identifies viral mRNA targets and reveals phenotypic effects in culture. Journal of Virology, 87: 6589-6603.

274. Whisnant, Adam W., Bogerd, Hal P., Flores, Omar, Ho, Phong, Powers, Jason G., Sharova, Natalia, Stevenson, Mario, Chen, Chin-Ho and Cullen, Bryan R. (2013) In depth analysis of the interaction of HIV 1 with cellular microRNA biogenesis and effector mechanisms. mBio, 4: e00193-13.

273. Wahl, Angela, Linnstaedt, Sarah D., Esoda, Caitlin, Krisko, John F., Martinez-Torres, Francisco, Delecluse, Henri-Jacques, Cullen, Bryan R. and Garcia, J. Victor (2013). A cluster of virus-encoded microRNAs accelerates acute systemic Epstein-Barr virus infection but does not significantly enhance virus-induced oncogenesis in vivo. Journal of Virology; 87: 5437-5446.

272. Cullen, Bryan R. (2013) Making a NeST for a persistent virus. Cell Host & Microbe; 13: 241-242.

271. Cullen, Bryan R. (2013) MicroRNAs as mediators of viral evasion of the immune system. Nature Immunology; 14: 205-210.


270. Cullen BR. (2012) MicroRNA expression by an oncogenic retrovirus. Proceedings of the National Academy of Sciences USA; 109(8): 2695-6.

269. Skalsky RL, Corcoran DL, Gottwein E, Frank CL, Kang D, Hafner M, Nusbaum JD, Feederle R, Delecluse H-J, Luftig MA, Tuschl T, Ohler U, and Cullen BR. (2012) The Viral and Cellular MicroRNA Targetome in Lymphoblastoid Cell Lines. PLoS Pathogens; 8(1): e1002484.


268. Gottwein E, Corcoran DL, Mukherjee N, Skalsky RL, Hafner M, Nusbaum JD, Shamulailatpam P, Love CL, Dave SS, Tuschl T, Ohler U, Cullen BR. (2011) Viral MicroRNA Targetome of KSHV-Infected Primary Effusion Lymphoma Cell Lines. Cell Host Microbe; 10(5): 515-26.

267. Zhang A, Bogerd H, Villinger F, Das Gupta J, Dong D, Klein EA, Jackett Jr. J, Schochetman G, Cullen BR, Silverman RH. (2011) In Vivo Hypermutation of Xenotropic Murine Leukemia Virus-Related Virus DNA in Peripheral Blood Mononuclear Cells of Rhesus Macaque by APOBEC3 Proteins. Virology;  5;421(1): 28-33.

266. Bryan R. Cullen. (2011) Herpesvirus microRNAs: Phenotypes and functions.Current Opinion in Virology; 1(3): 211-215.

265. Skalsky, Rebecca L. and Cullen, Bryan R. (2011) Reduced Expression of Brain-Enriched microRNAs in Glioblastomas Permits Targeted Regulation of a Cell Death Gene. PLoS ONE; 6(9): e24248.

264. Bryan R. Cullen. (2011) Viruses and microRNAs: RISCy interactions with serious consequences. Genes & Development; 25(18): 1881-94.

263. Feederle R, Haar J, Bernhardt K, Linnstaedt SD, Bannert H, Lips H, Cullen BR, Delecluse HJ. (2011) The members of a viral miRNA cluster co-operate to transform B lymphocytes.; Journal of Virology; 85(19): 9801-10.

262. Umbach JL & Cullen BR. Human alpha-herpesvirus microRNAs: Expression and functions. In: Weller SK, editor. Alpha Herpesviruses: Molecular and Cellular Biology: Horizon Scientific Press Caister Academic Press; 2011. p. 391-399.

261. Feederle R, Linnstaedt SD, Bannert H, Lips H, Bencun M, Cullen BR & Delecluse H-J. (2011) A viral microRNA cluster strongly potentiates the transforming properties of a human herpesvirus. PLoS Pathog; 7(2): e1001294.

260. Bogerd HP, Zhang F, Bieniasz PD & Cullen BR. (2011) Human APOBEC3 proteins can inhibit xenotropic murine leukemia virus-related virus infectivity. Virology; 410(1): 234-239.


259. Linnstaedt, Sarah D., Gottwein, Eva, Skalsky, Rebecca L., Luftig, Micah A., and Cullen, Bryan R. (2010). Virally induced cellular miR-155 plays a key role in B-cell immortalization by EBV. Journal of Virology; 84: 11670-11678.

258. Skalsky, Rebecca L. and Cullen, Bryan R. (2010). Viruses, microRNAs, and host interactions. Annual Review of Microbiology; 64: 123-141.

257. Umbach, Jennifer L., Yen, Hui-Ling, Poon, Leo L. M., and Cullen, Bryan R. (2010). Influenza A virus expresses high levels of a novel class of small viral leader RNAs in infected cells. mBio; 1: e00204-10.

256. Umbach, Jennifer L., Strelow, LI, Wong, Scott W., and Cullen, Bryan R. (2010). Analysis of rhesus rhadinovirus microRNAs Expressed in Virus-induced Tumors from Infected Rhesus Macaques, Virology; 405: 592-599.

255. Kelly, Elizabeth J., Cullen, Bryan R. and Russell, Stephen J. (2010). MicroRNA antagonism of the picornaviral life cycle: Alternative mechanisms of interference. PLoS Pathogens; 6: e1000820, doi:10.1371/journal.ppat.1000820.

254. Gottwein, Eva and Cullen, Bryan R. (2010). A human herpesvirus microRNA inhibits p21 expression and attenuates p21-mediated cell cycle arrest. Journal of Virology; 84: 5229-5237.

253. Cullen, Bryan R. (2010). Five questions about viruses and microRNAs. PLoS Pathogens; 6: e1000787. doi:10.1371/journal.ppat.1000787.

252. Skalsky, Rebecca L., Vanlandingham, Dana L., Scholle, Frank, Higgs, Stephen and Cullen, Bryan R. (2010). Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus. BMC Genomics; 11, 119.

251. Bogerd, Hal. P., Karnowski, Heather W., Cai, Xuezhong, Shin, Jinwook,, Pohlers, Michael and Cullen, Bryan R. (2010). A mammalian herpesvirus uses non-canonical expression and processing mechanisms to generate viral microRNAs. Molecular Cell; 37, 135-142.

250. Umbach, Jennifer L. and Cullen, Bryan R. (2010). In-depth analysis of KSHV microRNA expression provides insights into the mammalian microRNA processing machinery. Journal of Virology; 84, 695-703.

249. Umbach. Jennifer L., Wang, Kening, Tang, Shuang, Krause, Philip R., Mont, Erik K., Cohen, Jeffrey I. and Cullen, Bryan R. (2010) Identification of viral microRNAs expressed in human sacral ganglia latently infected with herpes simplex virus 2.Journal of Virology; 84, 1189-1192.


248. Umbach, Jennifer L., Nagel, Maria A., Cohrs, Randall J., Gilden, Donald H. and Cullen, Bryan R. (2009) Analysis of human α-herpesvirus microRNA expression in latently infected human trigeminal ganglia.; Journal of Virology; 83, 10677-10683.

247. Umbach, Jennifer L. and Cullen, Bryan R. (2009) The role of RNA interference and microRNAs in animal virus replication and antiviral immunity. Genes & Development; 23, 1151-1164.

246. Cullen, Bryan R. (2009) Viral RNAs: Lessons from the enemy. Cell; 136, 592-597.

245. Cullen, Bryan R. (2009) Viral and cellular messenger RNA targets of viral microRNAs. Nature; 457, 421-425.

242. Umbach, Jennifer Lin, Kramer, Martha F, Jurak, Igor, Karnowski, Heather W., Coen, Donald M. and Cullen, Bryan R. (2008) MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs. Nature; 454, 780-783.

241. Gottwein, Eva & Cullen, Bryan R. (2008) Viral and cellular microRNAs as determinants of viral pathogenesis and immunity. Cell Host & Microbe; 3, 375-387.


239. Gottwein, Eva, Mukherjee, Neelanjan, Sachse, Christoph, Frenzel Corina, Majoros, William H., Chi, Jen-Tsan A., Braich, Ravi, Manoharan, Muthiah, Soutschek, Jürgen, Ohler, Uwe , and Cullen, Bryan. R. (2007). A viral microRNA functions as an ortholog of cellular miR-155. Nature; 450, 1096-1099.

237. Lin, Jennifer and Cullen, Bryan R. (2007) Analysis of the interaction of primate retroviruses with the human RNA interference machinery. Journal of Virology; 81, 12218-12226.

234. Schäfer, Alexandra, Cai, Xuezhong, Bilello, John P., Desrosiers, Ronald C. and Cullen, Bryan R. (2007). Cloning and analysis of microRNAs encoded by the primate gamma-herpesvirus rhesus monkey rhadinovirus. Virology; 364, 21-27.

228. Cai, Xuezhong, Li, Gu, Laimins, Laimonis A. and Cullen, Bryan R. (2006). Human papillomavirus fenotype 31 does not express detectable microRNA levels during either latent or productive virus replication. Journal of Virology; 80, 10890-10893.

224. Cullen, Bryan R. (2006). Viruses and microRNAs. Nature Genetics; 38, S25-S30.

223. Cullen, Bryan R. (2006). Is RNA interference involved in intrinsic antiviral immunity in mammals? Nature Immunology; 7, 563-567.

222. Gottwein, Eva, Cai, Xuezhong and Cullen, Bryan R. (2006). A novel assay for viral microRNA function identifies a single nucleotide polymorphism that affects Drosha processing. Journal of Virology; 80, 5321-5326.

220. Cai, Xuezhong, Schäfer, Alexandra, Lu, Shihua, Bilello, John P., Desrosiers, Ronald C., Edwards, Rachel, Raab-Traub, Nancy and Cullen, Bryan R. (2006). Epstein-Barr virus microRNAs are evolutionarily conserved and differentially expressed. PLoS Pathogens; 2, e23.

218. Cai, Xuezhong and Cullen, Bryan R. (2006). Transcriptional origin of Kaposi’s sarcoma-associated virus microRNAs. Journal of Virology; 80, 2234-2242.


213. Zeng, Yan and Cullen, Bryan R. (2005). Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences. Journal of Biological Chemistry; 280, 27595-27603.

206. Cai, Xuezhong, Lu, Shihua, Zhang, Zhihong, Gonzalez, Carlos M., Damania, Blossom and Cullen, Bryan R. (2005). Kaposi’s sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells. Proceedings of the National Academy of Sciences USA; 102, 5570-5575.

205. Zeng, Yan, Yi, Rui and Cullen, Bryan R. (2005). Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. EMBO Journal; 24, 138-148.

201. Cullen, Bryan R. (2004). Transcription and processing of human microRNA precursors. Molecular Cell; 16, 861-865.

199. Cai, Xuezhong, Hagedorn, Curt H. and Cullen, Bryan R. (2004). Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA; 10, 1957-1966.

197. Zeng, Yan and Cullen, Bryan R. (2004). Structural requirements for pre-microRNA binding and nuclear export by Exportin 5. Nucleic Acids Research; 32, 4776-4785.


191. Yi, Rui, Qin, Yi, Macara, Ian G. and Cullen, Bryan R. (2003). Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes and Development; 17, 3011-3016.

190. Lee, Ming-Ta M., Coburn, Glen A., McClure, Myra O. and Cullen, Bryan R. (2003). Inhibition of human immunodeficiency virus type 1 replication in primary macrophages using Tat- or CCR5-specific small interfering RNAs expressed from a lentiviral vector.Journal of Virology; 77, 11964-11972.

186. Zeng, Yan, Yi, Rui and Cullen, Bryan R. (2003). MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proceedings of the National Academy of Sciences, USA; 100, 9779-9784.

180. Zeng, Yan and Cullen, Bryan R. (2003). Sequence requirements for microRNA processing and function in human cells. RNA; 9, 112-123.

178. Coburn, Glen A. and Cullen, Bryan R. (2002). Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. Journal of Virology; 76, 9225-9231.

177. Zeng, Yan and Cullen, Bryan R. (2002). RNA interference in human cells is restricted to the cytoplasm. RNA; 8, 855-860.

175. Zeng, Yan, Wagner, Eric J. and Cullen, Bryan R. (2002). Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Molecular Cell; 9, 1327-1333.