Projects

DrosophilaEmbryo-Wg • Finding new pathway components

Despite years of intensive study, we are still discovering new molecules that influence Wg/Wnt signaling. We have conducted a series of suppressor/enhancer screens using weak wg mutant alleles, and have isolated a number of modifiers that may help us identify new parts of the pathway or new control mechanisms.

Related papers:

• Greer, E. R., Chao, A.T., and Bejsovec, A. (2013) The role of Pebble/Ect2 in regulating Wg/Wnt activity. Development 140: 4937-46. PMID: 24198276

• Jones, W.M., Chao, A.T., Zavortink, M., Saint, R. and Bejsovec, A. (2010) Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. Journal of Cell Science 123: 2179-2189. PMID: 20516152

• Chao, A.T., Jones, W.M. and Bejsovec, A. (2007). The HMG-box transcription factor, Sox-Neuro, acts with Tcf to control Wg/Wnt signaling activity. Development 134: 989-997. PMID: 17267442

 

1wg • Transport of Wg within the epithelium

Wg is secreted from a single row of cells in each segment, but moves away from that row of cells to stabilize Arm and repress Svb across a broad domain in each embryonic segment. Wg, like other Wnts, has a fatty acyl group attached to it, so it associates with membranes and cannot freely diffuse. We want to understand how Wg can move from cell to cell to generate pattern.

Related papers:

• Moline, M. M., Southern, C. and Bejsovec, A. (1999). Directionality of Wingless protein transport is essential for wild-type epidermal patterning in the Drosophila embryo. Development 126, 4375-4384. PMID: 10477304

• Dierick, H. and Bejsovec, A. (1998). Functional analysis of Wingless reveals a link between intercellular ligand transport and dorsal-cell specific signaling. Development 125, 4729-4738. PMID: 9806921

 

WT SEM  • Generation of cell fate diversity

Low levels of Wg signaling within the denticle-secreting ventral domain generate the distinctive shapes in different rows of the denticle belts. We want to know how Wg target gene regulation produces these different cellular morphologies.

Related papers:

• Rizzo, N.P. and Bejsovec, A. (2017). SoxNeuro and Shavenbaby act cooperatively to shape denticles in the embryonic epidermis of Drosophila. Development 144: 2248-2258. PMID: 28506986

•  Bejsovec, A. and Chao, A.T. (2012) crinkled reveals a new role for Wingless signaling in Drosophila denticle formation. Development 139:690-698. PMID: 22219350

• Bejsovec, A. and Wieschaus, E. (1993). Segment polarity gene interactions modulate epidermal patterning in Drosophila embryos. Development 119, 501-517. PMID: 8287799

 

 

 

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