Despite years of intensive study, we are still discovering new molecules that influence Wg/Wnt signaling. We have conducted a series of suppressor/enhancer screens using weak wg mutant alleles, and have isolated a number of modifiers that may help us identify new parts of the pathway or new control mechanisms.
• Greer, E. R., Chao, A.T., and Bejsovec, A. (2013) The role of Pebble/Ect2 in regulating Wg/Wnt activity. Development 140: 4937-46. PMID: 24198276
• Jones, W.M., Chao, A.T., Zavortink, M., Saint, R. and Bejsovec, A. (2010) Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. Journal of Cell Science 123: 2179-2189. PMID: 20516152
• Chao, A.T., Jones, W.M. and Bejsovec, A. (2007). The HMG-box transcription factor, Sox-Neuro, acts with Tcf to control Wg/Wnt signaling activity. Development 134: 989-997. PMID: 17267442
Wg is secreted from a single row of cells in each segment, but moves away from that row of cells to stabilize Arm and repress Svb across a broad domain in each embryonic segment. Wg, like other Wnts, has a fatty acyl group attached to it, so it associates with membranes and cannot freely diffuse. We want to understand how Wg can move from cell to cell to generate pattern.
• Moline, M. M., Southern, C. and Bejsovec, A. (1999). Directionality of Wingless protein transport is essential for wild-type epidermal patterning in the Drosophila embryo. Development 126, 4375-4384. PMID: 10477304
• Dierick, H. and Bejsovec, A. (1998). Functional analysis of Wingless reveals a link between intercellular ligand transport and dorsal-cell specific signaling. Development 125, 4729-4738. PMID: 9806921
Low levels of Wg signaling within the denticle-secreting ventral domain generate the distinctive shapes in different rows of the denticle belts. We want to know how Wg target gene regulation produces these different cellular morphologies.
• Rizzo, N.P. and Bejsovec, A. (2017). SoxNeuro and Shavenbaby act cooperatively to shape denticles in the embryonic epidermis of Drosophila. Development 144: 2248-2258. PMID: 28506986
• Bejsovec, A. and Chao, A.T. (2012) crinkled reveals a new role for Wingless signaling in Drosophila denticle formation. Development 139:690-698. PMID: 22219350
• Bejsovec, A. and Wieschaus, E. (1993). Segment polarity gene interactions modulate epidermal patterning in Drosophila embryos. Development 119, 501-517. PMID: 8287799