{"id":4780,"date":"2021-06-22T23:13:12","date_gmt":"2021-06-23T04:13:12","guid":{"rendered":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/?p=4780"},"modified":"2021-06-22T23:13:12","modified_gmt":"2021-06-23T04:13:12","slug":"olfactory-sensations-regulation-of-chromatin-around-master-genes","status":"publish","type":"post","link":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/?p=4780","title":{"rendered":"Olfactory Sensation&#8217;s Regulation of Chromatin Around Master Genes"},"content":{"rendered":"<p><em>Drosophila<\/em> courtship behaviors are primarily governed by two master regulatory genes: <em>fruitless (fru)<\/em> and <em>doublesex (dsx). <\/em>Both of these genes undergo sex-specific splicing to produce male-specific transcription factors that drive male-specific courtship behaviors. In the olfactory system, there are three types of olfactory receptor neurons (ORNs) that express <em>fru<\/em> and regulate male-specific courtship behaviors: Or47b, Or67d and Ir84a. It is our hypothesis that olfactory sensation can alter courtship behavior by reprogramming the chromatin state around <em>fru<\/em> and <em>dsx<\/em>. In order to examine this hypothesis, Chromatin Immunoprecipitation (ChIP) using antibodies for RNA Polymerase II was performed on both Ir84a mutant and wild-type flies. Analysis of the qPCR results using <em>fru <\/em>primers from both mutant and wild-type flies suggest that **the chromatin conformation around <em>fru<\/em> in the Ir84a mutants was more closed than that of the wild type. This difference in chromatin state translates to a variance in expression rates of <em>fru<\/em> and thus influences the behavioral traits of the flies.<\/p>\n<p>**this is what the expected result is but there are two other potential results<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Drosophila courtship behaviors are primarily governed by two master regulatory genes: fruitless (fru) and doublesex (dsx). Both of these genes undergo sex-specific splicing to produce male-specific transcription factors that drive male-specific courtship behaviors. In the olfactory system, there are three types of olfactory receptor neurons (ORNs) that express fru and regulate male-specific courtship behaviors: Or47b, [&hellip;]<\/p>\n","protected":false},"author":43884,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[658455,658461],"tags":[],"class_list":["post-4780","post","type-post","status-publish","format-standard","hentry","category-bsurf-2021","category-week-6-bsurf-2021"],"_links":{"self":[{"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/posts\/4780","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/users\/43884"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4780"}],"version-history":[{"count":1,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/posts\/4780\/revisions"}],"predecessor-version":[{"id":4781,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=\/wp\/v2\/posts\/4780\/revisions\/4781"}],"wp:attachment":[{"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4780"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4780"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.duke.edu\/adhoc_org_trinity_hhr_fellows\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4780"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}