CV

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Education

Duke University, Durham, NC, USA (GPA 3.9/4) (2006-9/2012*)
Ph.D. candidate, Molecular Genetics and Microbiology

Novosibirsk State University, Novosibirsk, Russia (GPA 3.9/4) (2001-2006)
Honors specialist diploma (5 year degree), Molecular Biology

Research Experience

Graduate Research Assistant, Mentor: Matthias Gromeier, Ph.D. (2007 – present)
Duke University Medical Center, Durham, NC USA

Mechanism of inhibition of host translation during HSV-1 lytic infection

• Collaborating with other members of laboratory discovered HSV-1 proteins that interact with cellular translation initiation machinery.
• Analyzed subcellular distribution of host and viral proteins by immunofluorescence.

Control of eukaryotic translation initiation by MAPK signal transduction

• Utilized co-immunoprecipitation assays to investigate interaction of Mnk1 kinase with critical translation initiation factor eIF4G under a variety of physiological conditions.
• Created a panel of mutants to decipher the effect of phosphostatus and activity of Mnk1 on its binding to eIF4G.

Cdk1/cyclin B1 effect on protein synthesis in mitosis

• Employed phosphoproteomics to analyze the phosphorylation status of eIF4G in mitosis vs interphase.
• Investigated in vivo and in vitro phosphorylation of eIF4G by Cdk1/cyclin B1 kinase.
• Created inducible knock-out/knock-in cell lines to study the function of phosphomutant eIF4G.
• Currently investigating the effect of phosphorylation of eIF4G on mitotic translation utilizing metabolic labeling, luciferase reporters and polysomal profiling/qPCR.

Undergraduate Research Assistant, Mentor: Nina Tikunova, Ph.D. (2004 –2006)
Novosibirsk State University, Novosibirsk, Russia

• Applied phage display to select human recombinant antibodies to IL-18

Publications

• Dobrikov M, Dobrikova E, Shveygert M, Gromeier M. Phosphorylation of eIF4G1 by PKCα Regulates Interaction with Mnk1. Mol. Cell. Biol. 2010 Nov;30(21):5160-7
• Shveygert M, Bradrick S, Kaiser C, Gromeier M. Regulation of eIF4E phosphorylation by MAPK occurs through modulation of Mnk1-eIF4G interaction Mol. Cell. Biol. 2010 Nov;30(21):5160-7
• Goetz C, Dobrikova E, Shveygert M, Dobrikov M, Gromeier M. Oncolytic poliovirus against malignant glioma. Future Virology, Sep 2011, Vol. 6, No. 9, Pages 1045-1058.
  
• Dobrikova E*, Shveygert M*, Walters R, Gromeier M. Herpes simplex virus proteins ICP27 and UL47 associate with polyadenylate-binding protein and control its subcellular distribution. JVirol. 2010 Jan;84(1):270-9. *These authors contributed equally to this work
• Vikhrova MA, Shveygert MV, Khrapov EA, Filipenko ML, Gileva IP, Morozova VV, Tikunova NV.Selection of naturally occurring autoantibodies to interleukin-18 from phage display library. Hum Antibodies. 2010;19(2-3):71-8.
• Kaiser C, Dobrikova EY, Bradrick SS, Shveygert M, Herbert JT, Gromeier M. Activation of cap-independent translation by variant eukaryotic initiation factor 4G in vivo. RNA 2008 Oct;14(10):2170-82.
• Bradrick SS, Dobrikova EY, Kaiser C, Shveygert M, Gromeier M. Poly(A)-binding protein is differentially required for translation mediated by viral internal ribosomal entry site. RNA 2007 Sep;13(9):1582-93.

Awards and Fellowships

• Selected poster prize; Symposium on RNA biology, Durham, NC USA (2009)
• Best student oral presentation award; MGM retreat, Durham, NC USA (2009)
• Conference travel fellowship from Duke University Graduate School (2008, 2012)
• Diploma for the best talk at the XLIV International Scientific Conference Novosibirsk, Russia (2006)
• V. Potanin Federal Scholarship for Academic Excellence (2004-2006)

Meetings and Conferences

• Protein Translation and Cancer; Coronado, CA USA  (2010)
• Symposium on RNA Biology; Durham, NC USA (2009)
• Translational Control Meeting, CSHL, NY USA  (2008)
• XLIV International Scientific Student Conference; Novosibirsk, Russia (2006)

Teaching and Mentoring

Duke University

• Teaching assistant for General Microbiology course at Duke University (Fall 2010)
  Prepared and taught 2 labs/week for 16 students, graded tests, exams and final projects
•  Participated in ‘Teaching biology’ certificate program at Duke University (2010-2011)
•  Mentored medical student one-year research project at DUMC (2010-2011)

Service and Leadership

Volunteered at Duke International House (2007-2010)

• Prepared and facilitated 2-day orientation involving over 200 incoming international students
• Advised a group of 40 international student on accommodating to Duke campus and U.S. culture
• Assisted Cross-Cultural Counseling workshops

Languages

• English (fluent)
• Russian (native)
• Spanish (advanced)
• German (basic)