Epstein-Barr virus (EBV) is a human γ-herpesvirus that is able to establish a long-term, latent infection in human B cells for the life of the host. Some years ago, we discovered several microRNAs that are expressed by EBV in infected cells and we remain interested in how these microRNAs affect the pathogenicity and replication of EBV. To this end, we have published several papers that identified cellular mRNA targets for EBV microRNAs and showed how down regulation of these targets can facilitate EBV replication.